Effect of Nebivolol on the Isolated Rat Bladder Strips Precontracted with Carbachol or Potassium Chloride

Yıl 2020, Cilt: 10 Sayı: 3, 316 - 319, 29.09.2020

Hande Ozge Altunkaynak-camca

https://doi.org/10.33808/clinexphealthsci.731484

Öz

Amaç: Nebivololün vazorelaksan etkisine aracılık ettiği öne sürülen mekanizmalar arasında beta-2 ve/veya beta-3 adrenerjik reseptörler üzerindeki agonistik aktivitesi yer alır. Bu reseptörler, aynı zamanda mesanenin gevşemesiyle de ilişkilidir. Bu çalışmanın amacı, kolinerjik bir uyaran karbakol veya non-kolinerjik bir uyaran potasyum klorür (KCl) kullanılarak kasılmış izole sıçan mesane şeritlerinde nebivololün relaksan etkisini araştırmaktı.
Yöntem: İzole edilmiş mesane şeritleri organ banyosuna asıldı ve nebivolol (0.0001-100 µM) kümülatif olarak eklenmeden önce KCl (40 mM) veya karbakol (1 µM) ile mesane şeritleri kasıldı. Bu yanıtta beta-adrenerjik reseptörlerin rolünü araştırmak için bazı mesane şeritleri propranolol (1 µM) ile 30 dakika boyunca inkübe edildi. Karşılaştırmalarda Student’s t-testi kullanıldı. p<0.05 istatistiksel olarak anlamlı kabul edildi.
Bulgular: Nebivolol KCl veya karbakol ile kasılmış mesane şeritlerinde konsantrasyon-bağımlı gevşeme yanıtına yol açtı. Propranolol varlığında, karbakolle kasılmış izole sıçan mesane şeritlerindeki nebivololün relaksan etkisi anlamlı olarak (p<0.05) inhibe edilmesine karşın KCl ile kasılmış mesane şeritlerinde nebivololün relaksan etkisi propranolol ile inhibe edilemedi. Karbakolle kasılan mesane şeritlerinde nebivolole verilen maksimum yanıt KCl ile kasılanlarla kıyasla anlamlı olarak daha yüksek bulundu (p<0.05).
Sonuç: Bu çalışmanın bulguları, karbakolle kasılmış izole mesane şeritlerinde nebivololün relaksan etkisinde beta-adrenerjik reseptörlerin rolü olduğuna işaret etmektedir.

Anahtar Kelimeler

Nebivolol, relaksasyon, karbakol, potasyum klorür, mesane

Effect of Nebivolol on the Isolated Rat Bladder Strips Precontracted with Carbachol or Potassium Chloride

Öz

Objective: One of the proposed mechanism mediates the vasorelaxant effect of nebivolol is based on its agonistic activity on beta-2 and/or beta-3 adrenergic receptors. These receptors are also involved in the relaxation of urinary bladder. The aim of this study was to explore that the ability of nebivolol to induce relaxation of the isolated rat bladder strip precontracted with cholinergic stimuli using with carbachol or non-cholinergic stimuli using with potassium chloride (KCl).
Methods: The isolated bladder strips were mounted in organ bath and contracted by KCl (40 mM) or carbachol(1 µM) before the cumulative addition of nebivolol (0.0001-100 µM). To investigate the role of beta-adrenergic receptors in the nebivolol-induced relaxant response, some bladder strips were incubated with propranolol (1 µM) for 30 min. Statistical significance was tested by Student’s t-test. p<0.05 was considered to be statistically significant.
Results: Nebivolol elicited concentration-dependent relaxant response in the bladder strips precontracted with KCl or carbachol. Although the relaxant response to nebivolol in the bladder strips precontracted with carbachol was significantly inhibited by propranolol(p<0.05), the nebivolol-induced relaxation was failed to be inhibited by propranolol in the bladder strips precontracted with KCl. The maximum relaxation in response to nebivolol was found to be significantly higher in the bladder strips precontracted with carbachol compared to that of KCl (p<0.05).
Conclusion: The findings of the present study indicate that beta-adrenergic receptors play role in the relaxant response of nebivolol in the isolated rat bladder strip precontracted with carbachol.

Anahtar Kelimeler

Nebivolol, relaxation, carbachol, potassium chloride, bladder

Kaynakça

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