Preliminary Findings of the Distribution of Alpha-2-Adrenergic Receptor (ADRA2A) rs1800544 Polymorphisms in Kickboxing Players

Yıl 2021, Cilt: 11 Sayı: 4, 742 - 745, 26.10.2021

Başak Funda Eken , Ayşe Aysun Savci , Tolga Polat , Özlem Özge Yılmaz , Çisem Şılar , Beste Tacal Aslan , Şehkar Oktay , Korkut Ulucan

https://doi.org/10.33808/clinexphealthsci.863591

Öz

Objective: Adrenergic receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and adrenergic neurons in the central nervous system. The rs1800544 polymorphism in 3’-untranslated region of ADRA2A is caused by C-1291>G nucleotide change. In this study, we aimed to analyze the rs1800544 distribution in kickboxing players.

Methods: A total of 12 male players and 101 sedentary individuals (as a control group) participated in the study. Following DNA isolation, rs1800544 polymorphism of ADRA2A was determined by real-time polymerase chain reaction (Rt-PCR). Statistical analysis was carried out by chi-square test.

Results: CC, CG and GG genotypes and percentages for rs1800544 polymorphism were analyzed as 5 (41,7%), 4 (33,3%) and 3 (25%), respectively. C allele was counted as 14 (58,3%) and G allele as 10 (41,7%). In the control group CC, CG and GG were found as 34 (33,6%), 53 (52,5%), 14(13,9%), respectively. C allele was counted as 121 (60%) and G allele as 81 (40%). When we compare athletes and controls in the terms of genotypes, we detected no statistically significant difference (p>0,3940). For the allele comparison, we detected no significant difference between groups (p>0,0871).

Conclusion: Determination of gene combinations in Turkish athletes is of great importance for carrying out of these studies. Therefore, we aimed to determine the distribution of the alleles related with athletic performance on successful athletes. In our cohort, CC genotype was more frequently found, and C allele was also higher in players. We think that the effect of ADR2A2 gene on athletic performance might be determined more clearly by the fact that this study is an example of similar studies involving higher numbers of subjects.

Anahtar Kelimeler

ADRA2A, polymorphism, sports, kickboxing players

Kaynakça

• [1] Ulucan K. Literature review of Turkish sportsmen in terms of ACTN3 R577X polymorphism. Clinical and experımental health sciences 2016; 6, 44-47
• [2] Corak A, Kapıcı S, Sercan C, Akkoç O, Ulucan K. A pilot study for determination of anxiety related SLC6A4 promoter "S" and "L" alleles in healthy Turkish athletes. Cellular and Molecular Biology 2017; 63(5): 29-31.
• [3] Lightfoot JT, Hubal MJ, & Roth SM. (Eds.). Routledge Handbook of Sport and Exercise Systems Genetics 2019; Routledge.
• [4] Wang T, Heianza Y, Sun D, Huang T, Ma W, Rimm EB, & Qi L. Improving adherence to healthy dietary patterns, genetic risk, and long term weight gain: gene-diet interaction analysis in two prospective cohort studies. Bmj 2018; 360, 5644.
• [5] Zazryn TR, Finch CF, McCrory PA. 16 year study of injuries to professional kick boxers in the state of Victoria, Australia. Br J Sports Med 2003; 37: 448-451.
• [6] Kobilka B. Adrenergic receptors as models for G protein-coupled receptors. Annu Rev Neurosci 1992;15:87-114.
• [7] Bylund DB. Subtypes of α1‐ and α2‐radrenergic receptors. FASEB J6 1992;832‐839.
• [8] Hein Lutz, Altman John D, Kobilka Brian K. "Two functionally distinct α2-adrenergic receptors regulate sympathetic neurotransmission". Nature1999; 402 (6758):181–184. doi:10.1038/46040. PMID 10647009. S2CID 205047992.
• [9] da Silva TL, Pianca TG, Roman T, Hutz MH, Faraone SV, Schmitz M, et al. Adrenergic alpha2A receptor gene and response to methylphenidate in attention-deficit/hyperactivity disorder-predominantly inattentive type. J Neural Transm (Vienna) 2008;115:341–345.
• [10] Eken BF, Gezmiş H, Sercan C, Kapıcı S, Chousein ÖM, Kıraç D, Ulucan K. Türk Atletlerde D Vitamini Reseptör Geni Fok1 (rs2228570) ve Bsm1 (rs1544410) Polimorfizmlerinin Analizi. İstanbul Gelişim Üniversitesi Sağlık Bilimleri Dergisi 2018;(6): 561-572.
• [11] Arica S, Solgun HA, Dengi AS, Bakmaz E, Akçay T, Kapici S, Sercan, C, Ulucan K. −174 G/C polymorphism of interleukin 6 gene is not significantly different in Turkish professional short and long distance runners. Cell Mol Biol (Noisy le Grand) 2018; 64 (11): 85-87.
• [12] Bıyık B, Kapıcı S, Sercan C, Kulaksız H, Yüksel İ, Ulucan, K. Angiontensin Converting Enzyme Insertion/ Deletion Polymorphism Profile Of Turkish Professional Hip-Hop And Latin Dancers. Pamukkale Journal Of Sport Sciences 2018; 9(3):49-54.
• [13] Tsianos GI, Evangelou E, Boot A, Zillikens MC, van Meurs JB, Uitterlinden AG et al. Associations of polymorphisms of eight muscle- or metabolism-related genes with performance in Mount Olympus marathon runners. J Appl Physiol 2010; 108:567-574.
• [14] Wolfarth B, Rivera MA, Oppert JM, Boulay MR, Dionne FT, Chagnon M, Gagnon J, Chagnon M, Perusse L, Keul J, Bouchard C. A polymorphism in the alpha2a-adrenoceptor gene and endurance athlete status. Med Sci Sports Exercise 2000; Oct; 32(10):1709-12. doi: 10.1097/00005768-200010000-00008.