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The Effect of Bilirubin on Laboratory Investigations on Serum Creatinine: A Comparison Study Between Jaffe Reaction and Creatinase Enzymatic Method With Creatinine in Phosphate Buffered Saline Solution and Serum

Yıl 2022, Cilt: 12 Sayı: 1, 11 - 17, 30.03.2022
https://doi.org/10.33808/clinexphealthsci.714844

Öz

Objective: To determine the creatinine concentration in phosphate buffered saline solution and serum with different bilirubin concentrations
using Jaffe reaction and Creatinase method.
Methods: In Phase 1, creatinine and bilirubin concentrations in the dilution series were 0, 1, 2, 3, 4, 5, 6, 7, 8, 9 mg/dL and 0, 3, 6, 9, 12, 15,
18, 21, 24, 27, 30 mg/dL, respectively. Each creatinine concentration was spiked with eleven bilirubin concentrations used in Phase 1. In Phase
2, serum with creatinine values 0.51, 2.41 and 7.33 mg/dL were spiked with 11 bilirubin concentrations. The total bilirubin, creatinine by Jaffe
reaction and Creatinase method were measured.
Results: In Phase 1, Jaffe reaction showed a significant underestimation up to creatinine concentration of 2 mg/dL at all bilirubin concentrations.
From 3 mg/dL onwards, a significant overestimation was observed with high bilirubin concentrations. In Phase 2, Creatinase method gave no
significant underestimation in serum with 0.51 mg/dL of creatinine. But Jaffe reaction showed a significant underestimation from bilirubin
concentration of 7.18 mg/dL. In serum with 2.41 mg/dL creatinine, Creatinase and Jaffe methods gave significant underestimations from
bilirubin concentrations of 9.05 and 5.64 mg/dL, respectively. In serum of 7.33 mg/dL creatinine, significant underestimations were given from
bilirubin levels of 3.6 and 8.18 mg/dL by Creatinase and Jaffe methods, respectively.
Conclusion: In normal to moderately high bilirubin concentrations the Creatinase method is more accurate than the Jaffe method in all
creatinine concentrations used. At high creatinine concentrations Creatinase method gave significant underestimations which increased with
bilirubin concentration.

Destekleyen Kurum

University of Sri Jayewardenepura

Proje Numarası

MLS/19/17

Teşekkür

Mrs. Thilini Wijeratne

Kaynakça

  • [1] Vasudevan DM, Sreekumari S, Vaidyanathan K. Textbook of Biochemistry for Medical Students. 7th ed: Jaypee Brothers Medical Publishers (P) Ltd; 2013.
  • [2] Lolekha PH, Jaruthunyaluck S, Srisawasdi P. Deproteinization of serum: Another best approach to eliminate all forms of bilirubin interference on serum creatinine by the kinetic Jaffe reaction. J Clin Lab Anal 2001;15:116–121.
  • [3] Kroll MH, McCudden CR. Endogenous Interferences in Clinical Laboratory Tests. Walter de Gruyter GmbH; 2013.
  • [4] Cobbaert CM, Baadenhuijsen H, Weykamp CW. Prime time for enzymatic creatinine methods in pediatrics. Clin Chem 2009;55(3): 549-558.
  • [5] Marakala V, Avinash SS, Shivashankara AR, Malathi M, Kumar A. Serum creatinine assay: enzymatic vs kinetic Jaffe’s Method. J Evol Med Dent Sci 2012;1(4):328–334.
  • [6] Robert J, McEnroe P, Burritt P, Powers P, Douglas W, Rheinheimer MT, Brian H, Wallace P. Interference Testing in Clinical Chemistry; Approved Guideline. 2nd ed: Clinical and Laboratory Standards Institute; 2005.
  • [7] O’Leary N, Pembroke A, Duggan PF. A simplified procedure for eliminating the negative interference of bilirubin in the Jaffe reaction for creatinine. Clin Chem 1992;38(9):1749–1751.
  • [8] Dimeski G. Interference testing. Clin Biochem Rev 2008;29(1): 43-48.
  • [9] James O. Westgard, Elsa F. Quam. Interference and Recovery Experiments Westgard QC. 2018
  • [10] Chung H, Chun S, Min W. Creatinine determination with minimized interference. J Lab Med Qual Assur 2008;30(2):229– 231.
  • [11] Piyophirapong S, Wongtiraporn W, Sribhen K. Factitious results in clinical chemistry tests caused by common endogenous interferents. Siriraj Med J. 2010;62:185–188.
  • [12] Owen LJ, Wear JE, Keevil BG. Validation of a liquid chromatography tandem mass spectrometry assay for serum creatinine and comparison with enzymatic and Jaffe methods. Ann Clin Biochem 2006;43:118-123.
  • [13] Peake M, Whiting M. Measurement of serum creatinine – Current status and future goals. Clin Biochem Rev 2006;27(4):173–184.
  • [14] Yoshizawa T. Handbook of Optical Metrology: Principles and Applications. 2nd ed: CRC Press; 2017.
  • [15] Schmidt RL, Straseski JA, Raphael KL, Adams AH, Lehman CM. A Risk assessment of the Jaffe vs enzymatic method for creatinine measurement in an outpatient population. PLoS One 2015; DOI:10.1371/journal.pone.0143205
  • [16] Lolekha PH, Sritong N. Comparison of techniques for minimizing interference of bilirubin on serum creatinine determined by the kinetic Jaffé reaction. J Clin Lab Anal 1994;8:391–399.
  • [17] Knapp ML, Mayne PD. Development of an automated kinetic Jaffé method designed to minimise bilirubin interference in plasma creatinine assays. Clin Chim Acta 1987;168(2):239– 246.
Yıl 2022, Cilt: 12 Sayı: 1, 11 - 17, 30.03.2022
https://doi.org/10.33808/clinexphealthsci.714844

Öz

Proje Numarası

MLS/19/17

Kaynakça

  • [1] Vasudevan DM, Sreekumari S, Vaidyanathan K. Textbook of Biochemistry for Medical Students. 7th ed: Jaypee Brothers Medical Publishers (P) Ltd; 2013.
  • [2] Lolekha PH, Jaruthunyaluck S, Srisawasdi P. Deproteinization of serum: Another best approach to eliminate all forms of bilirubin interference on serum creatinine by the kinetic Jaffe reaction. J Clin Lab Anal 2001;15:116–121.
  • [3] Kroll MH, McCudden CR. Endogenous Interferences in Clinical Laboratory Tests. Walter de Gruyter GmbH; 2013.
  • [4] Cobbaert CM, Baadenhuijsen H, Weykamp CW. Prime time for enzymatic creatinine methods in pediatrics. Clin Chem 2009;55(3): 549-558.
  • [5] Marakala V, Avinash SS, Shivashankara AR, Malathi M, Kumar A. Serum creatinine assay: enzymatic vs kinetic Jaffe’s Method. J Evol Med Dent Sci 2012;1(4):328–334.
  • [6] Robert J, McEnroe P, Burritt P, Powers P, Douglas W, Rheinheimer MT, Brian H, Wallace P. Interference Testing in Clinical Chemistry; Approved Guideline. 2nd ed: Clinical and Laboratory Standards Institute; 2005.
  • [7] O’Leary N, Pembroke A, Duggan PF. A simplified procedure for eliminating the negative interference of bilirubin in the Jaffe reaction for creatinine. Clin Chem 1992;38(9):1749–1751.
  • [8] Dimeski G. Interference testing. Clin Biochem Rev 2008;29(1): 43-48.
  • [9] James O. Westgard, Elsa F. Quam. Interference and Recovery Experiments Westgard QC. 2018
  • [10] Chung H, Chun S, Min W. Creatinine determination with minimized interference. J Lab Med Qual Assur 2008;30(2):229– 231.
  • [11] Piyophirapong S, Wongtiraporn W, Sribhen K. Factitious results in clinical chemistry tests caused by common endogenous interferents. Siriraj Med J. 2010;62:185–188.
  • [12] Owen LJ, Wear JE, Keevil BG. Validation of a liquid chromatography tandem mass spectrometry assay for serum creatinine and comparison with enzymatic and Jaffe methods. Ann Clin Biochem 2006;43:118-123.
  • [13] Peake M, Whiting M. Measurement of serum creatinine – Current status and future goals. Clin Biochem Rev 2006;27(4):173–184.
  • [14] Yoshizawa T. Handbook of Optical Metrology: Principles and Applications. 2nd ed: CRC Press; 2017.
  • [15] Schmidt RL, Straseski JA, Raphael KL, Adams AH, Lehman CM. A Risk assessment of the Jaffe vs enzymatic method for creatinine measurement in an outpatient population. PLoS One 2015; DOI:10.1371/journal.pone.0143205
  • [16] Lolekha PH, Sritong N. Comparison of techniques for minimizing interference of bilirubin on serum creatinine determined by the kinetic Jaffé reaction. J Clin Lab Anal 1994;8:391–399.
  • [17] Knapp ML, Mayne PD. Development of an automated kinetic Jaffé method designed to minimise bilirubin interference in plasma creatinine assays. Clin Chim Acta 1987;168(2):239– 246.
Toplam 17 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Sağlık Kurumları Yönetimi
Bölüm Articles
Yazarlar

Ruchira Kumari Dissanayake Bu kişi benim 0000-0003-2926-7844

Kamburawala Kankanamgee Pramodya Tharaki Ranaweera Bu kişi benim 0000-0003-2538-0815

Pathiranage Dias Bu kişi benim 0000-0002-8330-0812

A.m.b. Priyadarshani 0000-0001-5721-5713

Proje Numarası MLS/19/17
Yayımlanma Tarihi 30 Mart 2022
Gönderilme Tarihi 5 Nisan 2020
Yayımlandığı Sayı Yıl 2022 Cilt: 12 Sayı: 1

Kaynak Göster

APA Dissanayake, R. K., Ranaweera, K. K. P. T., Dias, P., Priyadarshani, A. (2022). The Effect of Bilirubin on Laboratory Investigations on Serum Creatinine: A Comparison Study Between Jaffe Reaction and Creatinase Enzymatic Method With Creatinine in Phosphate Buffered Saline Solution and Serum. Clinical and Experimental Health Sciences, 12(1), 11-17. https://doi.org/10.33808/clinexphealthsci.714844
AMA Dissanayake RK, Ranaweera KKPT, Dias P, Priyadarshani A. The Effect of Bilirubin on Laboratory Investigations on Serum Creatinine: A Comparison Study Between Jaffe Reaction and Creatinase Enzymatic Method With Creatinine in Phosphate Buffered Saline Solution and Serum. Clinical and Experimental Health Sciences. Mart 2022;12(1):11-17. doi:10.33808/clinexphealthsci.714844
Chicago Dissanayake, Ruchira Kumari, Kamburawala Kankanamgee Pramodya Tharaki Ranaweera, Pathiranage Dias, ve A.m.b. Priyadarshani. “The Effect of Bilirubin on Laboratory Investigations on Serum Creatinine: A Comparison Study Between Jaffe Reaction and Creatinase Enzymatic Method With Creatinine in Phosphate Buffered Saline Solution and Serum”. Clinical and Experimental Health Sciences 12, sy. 1 (Mart 2022): 11-17. https://doi.org/10.33808/clinexphealthsci.714844.
EndNote Dissanayake RK, Ranaweera KKPT, Dias P, Priyadarshani A (01 Mart 2022) The Effect of Bilirubin on Laboratory Investigations on Serum Creatinine: A Comparison Study Between Jaffe Reaction and Creatinase Enzymatic Method With Creatinine in Phosphate Buffered Saline Solution and Serum. Clinical and Experimental Health Sciences 12 1 11–17.
IEEE R. K. Dissanayake, K. K. P. T. Ranaweera, P. Dias, ve A. Priyadarshani, “The Effect of Bilirubin on Laboratory Investigations on Serum Creatinine: A Comparison Study Between Jaffe Reaction and Creatinase Enzymatic Method With Creatinine in Phosphate Buffered Saline Solution and Serum”, Clinical and Experimental Health Sciences, c. 12, sy. 1, ss. 11–17, 2022, doi: 10.33808/clinexphealthsci.714844.
ISNAD Dissanayake, Ruchira Kumari vd. “The Effect of Bilirubin on Laboratory Investigations on Serum Creatinine: A Comparison Study Between Jaffe Reaction and Creatinase Enzymatic Method With Creatinine in Phosphate Buffered Saline Solution and Serum”. Clinical and Experimental Health Sciences 12/1 (Mart 2022), 11-17. https://doi.org/10.33808/clinexphealthsci.714844.
JAMA Dissanayake RK, Ranaweera KKPT, Dias P, Priyadarshani A. The Effect of Bilirubin on Laboratory Investigations on Serum Creatinine: A Comparison Study Between Jaffe Reaction and Creatinase Enzymatic Method With Creatinine in Phosphate Buffered Saline Solution and Serum. Clinical and Experimental Health Sciences. 2022;12:11–17.
MLA Dissanayake, Ruchira Kumari vd. “The Effect of Bilirubin on Laboratory Investigations on Serum Creatinine: A Comparison Study Between Jaffe Reaction and Creatinase Enzymatic Method With Creatinine in Phosphate Buffered Saline Solution and Serum”. Clinical and Experimental Health Sciences, c. 12, sy. 1, 2022, ss. 11-17, doi:10.33808/clinexphealthsci.714844.
Vancouver Dissanayake RK, Ranaweera KKPT, Dias P, Priyadarshani A. The Effect of Bilirubin on Laboratory Investigations on Serum Creatinine: A Comparison Study Between Jaffe Reaction and Creatinase Enzymatic Method With Creatinine in Phosphate Buffered Saline Solution and Serum. Clinical and Experimental Health Sciences. 2022;12(1):11-7.

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