Preparation of Chitosan-Polyvinyl Prolidone (PVP) Hydrogels with Fibroblast Growth Factor (FGF) and Investigation of in Vitro Characteristics

Year 2022, Volume: 12 Issue: 2, 418 - 422, 30.06.2022

Murat Doğan

https://doi.org/10.33808/clinexphealthsci.972758

Cited By: 1

Abstract

ABSTRACT
Objective: In this study, it was aimed to make in vitro characterization of the formulations by preparing hydrogel formulations using chitosan, a biocompatible and natural polymer, and PVP, a synthetic polymer. In addition, the effects of hydrogels containing FGF on the proliferation of keratinocyte cells were investigated.
Methods: Within the scope of the study, hydrogels with different properties were prepared and their water absorption capacity, and viscosities were examined. In addition, the hardness, adhesiveness, cohesiveness, and elasticity properties of hydrogels were investigated. The 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyl tetrazolium bromide (MTT) test was applied to evaluate the toxicity of hydrogels on keratinocyte cell lines.
Results: It was observed that hydrogel formulations have high water absorption capacity and suitable viscosity values. In addition, the mechanical characterization results showed that the hydrogels have suitable mechanical properties. According to the results of in vitro cell culture studies, it has been observed that hydrogels stimulate the proliferation of keratinocyte cells.
Conclusion: Results showed that the mechanical properties of hydrogels containing FGF are suitable for application and according to the results of in vitro cell culture studies, hydrogels can be used in wound healing studies because they increase keratinocyte cell proliferation.

Keywords

Chitosan, In vitro characterization, Hydrogel, Cell proliferation

Thanks

In this study, the facilities and devices of Cumhuriyet University Faculty of Pharmacy, Pharmaceutical Technology Department Research Laboratory and Medicine Faculty Cancer Research Center were used.

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