Tuzluluğun Trichoderma harzianum'un Büyüme, Antagonistik Potansiyeli, Proteaz Aktivitesi ve Prolin İçeriği Üzerindeki Etkisi

Öz

Sürdürülebilir bitkisel üretimin artması ve faydalı mikroorganizmaların büyümesi için tuzluluk ana sınırlayıcı faktörlerden biridir. Biyokontrol ajanı olan Trichoderma harzianum izolatlarının tuzlu koşullar altında test edilmesi, biyo-priming yoluyla bitkilerdeki tuz stresini hafifletmek için daha uygun suşlar seçmek gereklidir. Bu çalışmada, 14 T. harzianum izolatının Macrophomina phaseolina’ ya karşı antagonistik kapasitesinin yanı sıra büyüme, proteaz aktivitesi ve prolin içeriği farklı tuz konsantrasyonları altında araştırıldı. Th15 ve Th18 dışındaki tüm izolatlar, ikili kültür analizinde 0 mM NaCl'de M. phaseolina'ya karşı % 85'in üzerinde bir inhibisyon sergiledi ve tuz konsantrasyonu arttıkça antagonistik kapasitelerinde belirgin bir düşüş gösterdi. Test edilen tüm izolatlarda artan tuz konsantrasyonlarına bağlı olarak koloni büyümelerinde istatistiksel açıdan anlamlı azalmalar gözlemlendi. Tüm izolatların 0 mM'deki büyümesi, 70 mM NaCl hariç diğer NaCl uygulamalarında büyük ölçüde daha fazlaydı. Artan tuz seviyesi ile proteaz aktivitesi de azalmıştır. İzolatlar, prolin içeriğinde olduğu gibi aynı tuzlulukta bile farklı miktarlarda proteaz üretimi gösterdiler. Proteaz enziminin aksine, prolin içeriği 240 mM NaCl'de önemli ölçüde artmıştır. Tuzluluk, T. harzianum izolatlarında büyüme, antagonistik kapasitesi, proteaz aktivitesi ve prolin içeriği bakımından önemli bir rol oynamıştır.

Anahtar Kelimeler

• Biyokontrol ajanı,Macrophomina phaseolina,litik enzim,fitopatojen

The Effect of Salinity on Growth, Antagonistic Potential, Protease Activity, and Proline Content of Trichoderma harzianum

Öz

Salinity is one of the major limiting factors for sustainable crop production and the growth of beneficial microorganisms. Screening of biocontrol agent Trichoderma harzianum isolates under saline conditions could be necessary to select more efficient strains to alleviate salt stress through biopriming in plants. The present study aimed to investigate the antagonistic capacity of 14 T. harzianum isolates against Macrophomina phaseolina as well as the growth, protease activity, and proline content under different concentrations of salt. All isolates except Th15 and Th18 exhibited an over 85% inhibition against M. phaseolina at 0 mM NaCl in c and showed a noticeable decline in their antagonistic capacity as salt concentration increased. Colony growth decreased with increasing salt concentrations in all isolates tested. The growth of all isolates at 0 mM was significantly higher than the other NaCl treatments except at 70 mM NaCl. Protease activity also declined with the increased level of salt. Isolates displayed a wide range of protease expression patterns even at the same salinity as in the case of proline content. Unlike protease enzyme, proline content significantly increased at 240 mM NaCl. Salinity played a significant role in T. harzianum isolates with regards to the growth, antagonistic capacity, protease activity, and proline content.

Anahtar Kelimeler

• Biocontrol agent,Macrophomina phaseolina,lytic enzyme,phytopathogen

Kaynakça

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