Determination of chemical content of Lemna minor L. by GC-MS and investigation of antioxidant activity

Year 2022, , 53 - 64, 30.06.2022

Sema Yiyit Doğan , Sibel Atasagun , Mehmet Borga Ergönül

https://doi.org/10.53447/communc.1122558

Cited By: 1

Abstract

Lemna minor L. has been traditionally used for a long time for its analgesic, antipyretic, vitamin C supplement, astringent, antipruritic effects. Although there are many heavy metal removals using L. minor, unfortunately, biological activity studies are very limited. In this study, the chemical content and total phenol content, DPPH removal, metal chelation (Fe2+) and β-Carotene-lycopene methods of the L. minor macrophyte we obtained from Turkey were determined by GC-MS. The results of the study showed that our plant contains 25 different essential oils and has a high phenol content. In addition, 72% DPPH removal of L.minor was determined when it had 71% iron chelating ability. As a result of our study, it has been revealed that the L. minor we use is an effective antioxidant. It is thought that its usability in the fields of food and medicine can be investigated with further studies.

Keywords

Lemna minor, antioxidants, DPPH, total phenol, GC-MS

Supporting Institution

Coordination of Scientific Research Projects of Ankara University

Project Number

21L0430004

Thanks

This work was financially supported by the Coordination of Scientific Research Projects of Ankara University (Research Project No: 21L0430004) in Turkey. We would like to thank Asst. Prof. Ayşegül H. MENDİ for her assistance.

References

• Yildirim, M., Degirmenci, U., Akkapulu, M., Comelekoglu, U., Balli, E., Ozcan, T. M., Yalin, S., The effect of Rheum ribes L. on oxidative stress in diabetic rats, Journal of Basic and Clinical Physiology and Pharmacology, 32(1) (2021). http://doi 10.1515/jbcpp-2020-0058
• Preci, D. P., Almeida, A., Weiler, A. L., Franciosi, M. L. M., Cardoso, A. M., Oxidative damage and antioxidants in cervical cancer, International Journal of Gynecologic Cancer, 31(2) (2021). http://dx.doi.org/10.1136/ijgc-2020-001587
• Garcia, C., Blesso, C. N., Antioxidant properties of anthocyanins and their mechanism of action in atherosclerosis, Free Radical Biology and Medicine, 172 (2021), 152-166. https://doi.org/10.1016/j.freeradbiomed.2021.05.040
• Karamalakova, Y., Stefanov, I., Georgieva, E., Nikolova, G., Pulmonary Protein Oxidation and Oxidative Stress Modulation by Lemna minor L. in Progressive Bleomycin-Induced Idiopathic Pulmonary Fibrosis. Antioxidants, 11(3) (2022), 523. http://doi 10.3390/antiox11030523.
• Zhang, P. Y., Xu, X., Li, X. C., Cardiovascular diseases: oxidative damage and antioxidant protection, European Review for Medical and Pharmacological Sciences, 18(20) (2014), 3091-3096.
• Koca, N., Karadeniz, F., Serbest radikal oluşum mekanizmaları ve vücuttaki antioksidan savunma sistemleri, Gıda Mühendisliği Dergisi, 16, (2003), 32-37.
• Rahal, A., Kumar, A., Singh, V., Yadav, B., Tiwari, R., Chakraborty, S., Dhama, K., Oxidative stress, prooxidants, and antioxidants: the interplay, BioMed Research International, (2014):761274. https://doi.org/10.1155/2014/761264
• Karabulut, H., Gülay, M. Ş., Antioksidanlar. Veterinary Journal of Mehmet Akif Ersoy University, 1 (1) (2016), 65-76.
• Simsek, I., Aytekin, F., Yesilada, E., Yildirimli, Ş., An ethnobotanical survey of the Beypazari, Ayas, and Güdül district towns of Ankara Province (Turkey). Economic botany, 58(4) (2004), 705-720. https://doi.10.1663/0013-0001(2004)058 [0705:AESOTB]2.0.CO;2
• Özhatay, N., Koyuncu, M., Atay, S., Byfield, A., Türkiye'nin doğal tıbbi bitkilerinin ticareti hakkında bir çalışma, Doğal Hayatı Koruma Derneği, (1997).
• Al-Snai, A. E., Lemna minor: Traditional uses, chemical constituents and pharmacological effects-A review, IOSR Journal of Pharmacy, 9(8) (2019), 6-11.
• González-Renteria, M., del Carmen Monroy-Dosta, M., Guzmán-García, X., Hernández-Calderas, I., Antibacterial activity of Lemna minor extracts against Pseudomonas fluorescens and safety evaluation in a zebrafish model, Saudi Journal of Biological Sciences, 27(12) (2020), 3465-3473. https://doi.org/10.1016/j.sjbs.2020.09.043
• Gulcin, I., Kirecci, E., Akkemik, E., Topal, F., Hisar, O., Antioxidant, antibacterial, and anticandidal activities of an aquatic plant: duckweed (Lemna minor L. Lemnaceae), Turkish Journal of Biology, 34(2) (2010), 175-188. http://doi. 10.3906/biy-0806-7
• Popov, S. V., A. Günter, E., Markov, P. A., Smirnov, V. V., Khramova, D. S., Ovodov, Y. S., Adjuvant effect of lemnan, pectic polysaccharide of callus culture of Lemna minor L. at oral administration, Immunopharmacology and Immunotoxicology, 28(1) (2006), 141-152. https://doi.org/10.1080/08923970600626098
• Yağcıoğlu, P., Farklı ekstraksiyon metotları ile adaçayı (Salvia officinalis L.) bitkisinden antioksidan ekstraksiyonunun optimizasyonu (Doctoral dissertation, Fen Bilimleri Enstitüsü), 2015.
• Barros, L., Ferreira, M. J., Queiros, B., Ferreira, I. C., Baptista, P., Total phenols, ascorbic acid, β-carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities, Food chemistry, 103(2) (2007), 413-419. https://doi.org/10.1016/j.foodchem.2006.07.038
• Blois, M. S., Antioxidant determinations by the use of a stable free radical, Nature, 181(4617) (1958), 1199-1200. https://doi.org/10.1038/1811199a0
• Decker, E. A., & Welch, B., Role of ferritin as a lipid oxidation catalyst in muscle food, Journal of Agricultural and food Chemistry, 38(3) (1990), 674-677.
• Nagata, M., Yamashita, I., Simple method for simultaneous determination of chlorophyll and carotenoids in tomato fruit, Nippon Shokuhin Kogyo Gakkaishi, 39(10) (1992), 925-928. https://doi.org/10.3136/nskkk1962.39.925
• Muniandy, K., Gothai, S., Tan, W. S., Kumar, S. S., Mohd Esa, N., Chandramohan, G., Arulselvan, P., In vitro wound healing potential of stem extract of Alternanthera sessilis, Evidence-based complementary and alternative medicine, (2018), http://doi10.1155/2018/3142073.
• Moraes, J., de Oliveira, R. N., Costa, J. P., Junior, A. L., de Sousa, D. P., Freitas, R. M., & Pinto, P. L., Phytol, a diterpene alcohol from chlorophyll, as a drug against neglected tropical disease Schistosomiasis mansoni, PLoS Neglected Tropical Diseases, 8(1) (2014), e2617. https://doi.org/10.1371/journal.pntd.00026 Varsha, K. K., Devendra, L., Shilpa, G., Priya, S., Pandey, A., Nampoothiri, K. M., 2, 4-Di-tert-butyl phenol as the antifungal, antioxidant bioactive purified from a newly isolated Lactococcus sp., International journal of food microbiology, 211 (2015), 44-50. https://doi.org/10.1016/j.ijfoodmicro.2015.06.025
• Petrova-Tacheva, V., Ivanov, V., & Atanasov, A., Lemna minor L. as a source of antioxidants, Trakia Journal of Science, 18 (2020), 157-162. http://doi 10.15547/tjs.2020.s.01.029
• Kim, Y., Hyun, S. H., Park, H. E., & Choi, H. K., Metabolic profiling, free-radical scavenging and tyrosinase inhibitory activities of Lemna minor whole plants cultivated in various concentrations of proline and sucrose, Process Biochemistry, 47(1) (2012), 62-68. https://doi.org/10.1016/j.procbio.2011.10.010
• Tran, H. C., Le, H. A. T., Le, T. T., Effects of Enzyme Types and Extraction Conditions on Protein Recovery and Antioxidant Properties of Hydrolysed Proteins Derived from Defatted Lemna minor, Applied Science and Engineering Progress, 14(3) (2021), 360-369. https://doi.org/10.14416/j.asep.2021.05.003
• Saritha, K., Saraswathi, U., Antioxidant activity of gold nanoparticles synthesized using Lemna minor, World Journal of Pharmaceutical Sciences, (2014), 1545-1551.
• Iskandar, I., Andriani, Y., Rostika, R., Zidni, I., & Riyanti, N. A., Effect of using fermented Lemna sp. in fish feed on growth rate of nilem carp (Osteochilus hasselti), World News of Natural Sciences, 26 (2019), 157-166.
• Baynal, Ö. F., Likopen, Erciyes Üniversitesi Eczacılık Fakültesi, Bitirme tezi, 2012.