BIOFILM FORMATION AND CONTROL OF FACULTATIVE THERMOPHILE BREVIBACILLUS AGRI D505B

Abstract

Brevibacillus agri D505b is an aerobic, non-pathogenic, endospore-forming facultative thermophilic bacillus. Six abiotic surfaces (stainless steel, glass, polyvinyl chloride, polypropylene, polystyrene, and polycarbonate) were compared with viable cell enumerations. According to results, D505b cells could able to attach all these surfaces. Stainless steel (6.10 log CFU/cm2) was found to be the most effective surface for biofilm formation. Polycarbonate (6.03 log CFU/cm2) was found as the second best surface. Furthermore, the D505b biofilm was treated with 15 different sanitation agents and trichloroacetic acid (TCA) was determined to be the most effective one (80.3% removal). Our results showed that the strong biofilm producer B. agri D505b was very resistant to various sanitation agents. However, TCA significantly inhibited biofilm formation for the isolate. 

Keywords

• Brevibacillus agri,biofilm control,abiotic surfaces,enzymes

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