ACCUMULATION OF CR6+, PB2+ AND CD2+ AND ULTRAVIOLET RADIATION ALTER METHYLATION AND GENOMIC DNA STATUS IN RAMALINA FARINACEAE

Yıl 2021, Cilt: 30 Sayı: 2, 98 - 118, 31.12.2021

Rasim Hamutoğlu Demet Cansaran Duman Mehmet Kürşat Derici Sumer Aras Ali Aslan

https://doi.org/10.53447/communc.915250

Öz

In this study was aimed to determine the genotoxic effect of Ramalina farinacea lichen species against stress sources at the molecular level. After applying three different heavy metals (Pb2+, Cd2+, and Cr6+) to the R. farinacea, the extent to which the lichen sample absorbed these metals was determined by Flame Atomic Absorption Spectroscopy. RAPD and MSAP-AFLP assays were also used to determine the status of DNA damage. The heavy metal analysis showed that R. farinacea had high levels of Pb2+, Cd2+, and Cr6+ content. According to the results obtained from molecular analyses, band changes were observed against seven primers heavy metal stresses and three primers against UV stress. An increase in Genomic Template Stability (GTS) was determined during the time in R. farinacea treated with all heavy metal concentrations. The effect of UV radiations in R. farinacea revealed the highest polymorphism and the lowest GTS rate depending on the dose. Among all methylation combinations, Type II was found to show altered in R. farinacea in response to Pb2+, Cd2+, and Cr6+ contents and UV radiations. R. farinacea can be used at the molecular level as a biomarker of suitable genotoxic effect. This is the first study to reveal DNA damage against stress sources using a sample of R. farinacea lichen species.

Anahtar Kelimeler

Ramalina farinacea, heavy metals, ultraviolet radiation, genotoxicity

Destekleyen Kurum

TUBITAK

Proje Numarası

112T004

Kaynakça

• Gunatilake, S.K., Methods of removing heavy metals from industrial wastewater, Methods, 1 (1) (2015), 14.
• Jarup, L., Hazards of heavy metal contamination, British Medical Bulletin, 68 (2003), 167–182.
• Sujetoviene, G., Sliumpaite, I., Response of Evernia prunastri transplanted to an urban area in central Lithuania, Atmospheric Pollution Research, 4 (2013), 222–228.
• Bačkor, M., Loppi, S., Interactions of lichens with heavy metals, Biologia Plantarum, 53 (2009), 214–222.
• Zulaini, A. A. M., Muhammad, N., Asman, S., Hashim, N. S., Jusoh, S., Abas, A., Yusof, H., Din, L., Evaluation of transplanted lichens, Parmotrema tinctorum and Usnea diffracta as bioindicator on heavy metals accumulation in Southern Peninsular Malaysia, Journal of Sustainability Science and Management, 14 (4) (2019) 1–13.
• Hamutoğlu, R., Aslan, A., Aras, S., Cansaran-Duman, D., Environmental risk assessment under the pollutants exposure with using four lichen species and molecular assay in cement plant, Aşkale-Erzurum (Turkey), Turk Hijyen ve Deneysel Biyoloji Dergisi, 73 (3) (2016) 253–266.
• Conti, M. E., Cecchetti, G., Biological monitoring: lichens as bioindicators of air pollution assessment-a review, Environmental Pollution, 114 (2001), 471–492.
• Seed, L., W olseley , P ., Gosling, L., Davies, L., Power , S. A., Modelling relationships between lichen bioindicators, air quality and climate on a national scale: results from the UK OPAL air survey, Environmental Pollution,182 (2013), 437–447.
• Sujetoviene, G. Monitoring lichen as indicators of atmospheric quality. In: Upreti, D. K. Editor. Recent Advances in Lichenology. Springer, India, (2015), 87–118.
• Sujetoviene, G., Smilgaitis, P., Dagiliute, R., Zaltauskaite, J., Metal accumulation and physiological response of the lichens transplanted near a landfill in central Lithuania, Waste Management, 85 (2019), 60–65.
• Purvis, O. W., Halls, C., A review of lichens in metal-enriched environments, Lichenologist, 28 (1996), 571–601.
• Hauck, M., Dulamsuren, C., Muhlenberg, M., Lichen diversity on steppe slopes in the northern Mongolian mountain taiga and its dependenceon microclimate, Flora, 202 (2007), 530–546.
• Hauck, M., Paul, A., Manganese as a site factor for epiphytic lichens, Lichenologist, 37 (2005), 409–423.
• Pescott, O. L., Simkin, J. M., August, T. A., Randle, Z., Dore, A. J., Botham, M. S., Air pollution and its effects on lichens, bryophytes, and lichen-feeding Lepidoptera: Review and evidence from biological records, Biological Journal of the Linnean Society, 115 (2015), 611–635.
• Ares, Á., Itouga, M., Kato, Y., Sakakibara, H., Differential Metal Tolerance and Accumulation Patterns of Cd, Cu, Pb and Zn in the Liverwort Marchantia polymorpha L, Bulletin of Environmental Contamination and Toxicology, 100 (2017), 444–450.
• Hamutoğlu, R., Derici, M. K., Aras, E. S., Aslan, A., Cansaran-Duman, D., The physiological and DNA damage response of in the lichen Hypogymnia physodes to UV and heavy metal stress, Applied Ecology and Environmental Research, 18 (2) (2020), 2315–2338.
• Frohnmeyer, H., Staiger, D., Ultraviolet-B radiation-mediated responses in plants, Plant Physiology, 133 (2003), 1420–1428.
• Liu, H. J., Zhang, J. L., Zhang, F. S., Role of iron plaque in Cd uptake by and translocation within rice (Oryza sativa L.) seedlings grown in solution culture, Environmental and Experimental Botany, 59 (3) (2007), 314–320.
• Cockell, C. S., Knowland, J., Ultraviolet radiation screening compounds, Biological Reviews of the Cambridge Philosophical Society, 74 (1999), 311–345.
• Al-Amoody, A. A., Yayman, D., Kaan, T., Özkök, E. A., Özcan, A., Özen, E., Çobanoğlu Yiğitoğlu, G., Role of lichen secondary metabolites and pigments in UV- screening phenomenon in lichens, Acta Biologica Turcica, 33 (1) (2020), 35–48.
• Hill, D. J., Woolhouse, H. W., Aspects of the autecology of Xanthoria parietina agg, Lichenologist, 3 (1966), 207–214.
• Trest, M. T., Will-wolf, S., Keuler, R., Shay, N., Hill, K., Studer, A., Muench, A., Alexander, Z., Adams, A., Dittberner, L., Feehan, M., Lee, H., Galleguillos-katz, N., Zedler, J. B., Graham, L., Arancibia-avila, P., Potential impacts of uv exposure on lichen communities: a pilot study of nothofagus dombeyi trunks in southernmost chile, Ecosystem Health and Sustainability, 1 (2015), 1–12.
• Hall, R. B. S,, Bornman, J. F., Björn, L. O., UV-induced changes in pigment content and light penetration in the fruticose lichen Cladonia arbuscula ssp. mitis, Journal of Photochemistry and Photobiology, 66 (1) (2002), 13-20.
• Conte, C., Mutti, I., Puglisi, P., Ferrarini, A., Regina, G., Maestri, E., Manmiroli, N., DNA fingerprint analysis by PCR based method for monitoring the genotoxic effects of heavy metals pollution, Chemosphere, 37 (1998), 2739–2749.
• Savva, D., The use of Arbitrarily primed PCR (AP-PCR) fingerprinting detects exposure to genotoxic chemicals, Ecotoxicology, 9 (2000), 341–353.
• Citterio, S., Aina, R., Labra, M., Ghiani, A., Fumagalli, P ., Sgorbati, S., Santagostino, A., Soil genotoxicity: a new strategy based on biomolecular tools and plants bioindicators, Environmental Science & Technology, 36 (2002), 2748–2753.
• Körpe, D. A., Aras, S., Evaluation of copper-induced stress on eggplant (Solanum melongena L.) seedlings at the molecular and population levels by use of various biomarkers, Mutation Research, 719 (1–2) (2011), 29–34.
• Muller, L. A. H., Lambaerts, M., Vangronsveld, J., Colpaert, J. V., AFLP-based assessment of the effects of environmental heavy metal pollution on the genetic structure of pioneer populations of Suillus luteus, New Phytologist, 164 (2) (2004), 297–303.
• Liu, W., Li, P. J., Qi, X. M., Zhou, Q. X., Zheng, L., Sun, T. H., Yang, Y. S., DNA changes in barley (Hordeum vulgare) seedlings induced by cadmium pollution using RAPD analysis, Chemosphere, 61 (2) (2005), 158–167.
• Cansaran-Duman, D., Atakol, O., Aras, S., Assessment of air pollution genotoxicity by RAPD in Evernia prunastri L. Ach. from around iron-steel factory in Karabuk, Turkey, Journal of Environmental Science (China)., 23 (7) (2011), 1171–1178.
• Aydin, S. S., Gokce, E., Buyuk, I., Aras, S., Characterization of stress induced by copper and zinc on cucumber (Cucumis sativus L.) seedlings by means of molecular and population parameters, Mutation Research, 746 (1) (2012), 49–55.
• Vardar, C., Basaran, E., Cansaran-Duman, D., Aras, S., Air-quality biomonitoring: assessment of genotoxicity of air pollution in the Province of Kayseri (Central Anatolia) by use of the lichen Pseudevernia furfuracea (L.) Zopf and amplified fragment-length polymorphism markers, Mutation Research, 759 (2014), 43–50.
• Lukens, L. N., Zhan, S., The plant genome's methylation status and response to stress: implications for plant improvement, Current Opinion in Plant Biology, 10 (3) (2007), 317–322.
• Singh, N. P., McCoy, M. T., Tice, R. R., Schneider, E. L., A simple technique for quantitation of low levels of DNA damage in individual cells, Experimental Cell Research, 175 (1988), 184–191.
• Dhawan, A., Bajpayee, M., Parmar, D., Comet assay: a reliable tool for the assessment of DNA damage in different models, Cell Biology & Toxicology, 25 (2009), 5–32.
• Bajpayee, M., Kumar, A., Dhawan, A., The comet assay: assessment of in vitro and in vivo DNA damage, Methods in Molecular Biology, 1044 (2013), 325–345.
• Aras, S., Cansaran-Duman, D., Isolation of DNA for sequence analysis from herbarium material of some lichen species, Turkish Journal of Botany, 30 (2006), 449–453.
• Atienzar, F. A., Conradi, M., Evenden, A. J., Jha, A. N., Depledge, M. H., Qualitative assesment of genotoxicity using random amplified polymorphic DNA: comparison of genomic template stability with key fitness parameters in Daphnia magna exposed to benzo[a]pyrene, Environmental Toxicology and Chemistry, 18 (1999), 2275–2282.
• Li, M., Liu, Z., Xu, Y., Cui, Y., Li, D., Kong, Z., Comparative effects of Cd and Pb+2 on biochemical response on DNA damage in the earthworm Eisenia foetida (Annelida, Oligochaeta), Chemosphere, 74 (2009), 621–625.
• Kurelec, B., The genotoxic diseases syndrome, Marine Environmental Research, 35 (1993), 341–348.
• Reinecke, S. A., Reinecke, A. J., The comet assay as biomarker of heavy metal genotoxicity in Earthworms, Environmental Contamination & Toxicology, 46 (2004), 208–215.
• Sorrentino, M. C., Capozzi, F., Giordano, S., Spagnuolo, V., Genotoxic effect of Pb and Cd on in vitro cultures of Sphagnum palustre: An evaluation by ISSR markers, Chemosphere, 181 (2017), 208–215.
• Batir, M. B., Candan, F., Buyuk, I., Aras, S., The determination of physiological and DNA changes in seedlings of maize (Zea mays L.) seeds exposed to the waters of the Gediz River and copper heavy metal stress, Environmental Monitoring and Assessment, 187 (2015), 169.
• Bajpai, R., Shukla, V., Singh, N., Rana, T. S., Upreti, D. K., Physiological and genetic effects of chromium (+VI) on toxitolerant lichen species, Pyxine cocoes, Environmental Science and Pollution Research, 22 (2015), 3727–3738.
• Gill, R. A., Zang, L., Ali, B., Farooq, M. A., Cui, P., Yang, S., Ali, S., Zhou, W., Chromium induced physio-chemical and ultrastructural changes in four cultivals of Brassica napus L, Chemosphere, 120 (2015), 154–164.
• Sulaiman, N., Fuzy, S. F. F. M., Muis, S. I. N. A., Ismail, B. S., Use of lichens as bioindicators for determining atmospheric heavy metal concentration in Malasia, Pakistan Journal of Botany, 50 (1) (2018), 421–428.
• Sujetoviene, G., Česynaitė, J., Assessment of air pollution at the indoor environment of a shooting range using lichens as biomonitors, Journal of Toxicology and Environmental Health, Part A, 84 (7) (2021), 273–278.
• Chetia, J., Gogoi, N., Gogoi, R., Yasmin, F., Impact of the heavy metals on physiological health of lichens growing in differently polluted areas of central Assam, North East India, Plant Physiology Reports, (2021), 1–10.
• Rastogi, R. P., Kumar, A., Tyagi, M. B., Sinha, R. P., Molecular mechanisms of ultraviolet radiation-induced DNA damage and repair, Journal of Nucleic Acids., (2010), 1–32.
• Hollosy, F., Effects of ultraviolet radiation on plant cells, Micron, 33 (2) (2002), 179–197.
• Atienzar, F. A., Cordi, B., Donkin, M. E., Evenden, A. J., Jha, A. N., Depledge, M. H., Comparison of ultraviolet-induced genotoxicity detected by random amplified polymorphic DNA with chlorophyll fluorescence and growth in a marine macroalgae, Palmaria palmata, Aquatic Toxicology, 50 (2000), 1–12.
• Garty, J., Weissman, L., Levin, T., Garty-Spitz, R., Lehr, H., Impact of UV-B, Heat and Chemicals on Ethylene-Production of Lichens, Journal of Atmospheric Chemistry, 49 (2004), 251–266.