Genetic Diversity of Cotton Bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae) Population in Çanakkale

Yıl 2022, Cilt: 10 Sayı: 1, 211 - 221, 25.07.2022

Seda Yücel Hanife Genç

https://doi.org/10.33202/comuagri.1054611

Cited By: 1

Öz

The cotton bollworm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) is a commonly known polyphagous pest in agricultural areas all over the world. It has been a major pest causing significant damages by reducing tomato fruit production and quality since 1913 in Turkey. The objective of the study was to evaluate genetic diversity and phylogenetic relationship of H. armigera based on mitochondrial COI gene. Infested tomatoes with H. armigera larvae were collected from Çanakkale province (14 location), Balıkesir (1 location) and Bursa (1 location) in August and September 2017 and 2018. Larvae representing for each location (n=3) were used for genomic DNA isolation. The partial COI gene (536 bp) was amplified and PCR products were sequenced directly. The numbers of haplotypes and mutations were estimated using the DnaSP. Genetic relationships between the population were evaluated using the UPGMA method in MEGA X program. As a result, we identified 13 haplotypes and 13 mutations in all tested 45 samples. Additionally, 9 private haplotypes were detected among H. armigera population. Analyses revealed that population of H. armigera sampled from different geographic location were established as two distinct groups. The first group comprised 12 haplotypes (Çanakkale and Balıkesir population) while the distinct second group consisted of only 1 haplotype (Bursa population). The presented study was the first attempt to detect molecular characterization and genetic characterization of cotton bollworm in Turkey.

Anahtar Kelimeler

Helicoverpa armigera, Cotton bollworm, Genetic diversity, mtDNA, COI gene

Destekleyen Kurum

Çanakkale Onsekiz Mart Üniversitesi BAP

Proje Numarası

FYL-2018/2466

Teşekkür

This research was financially supported by Canakkale Onsekiz Mart University, Scientific Research Projects Commission (FYL-2018/2466). The authors thank Emeritus Prof. Dr. James L. Nation for reviewing and his useful suggestions. The part of this study was published as an abstract in the 1st International Molecular Plant Protection Congress, April 10-13, 2019 in Adana, Turkey.

Çanakkale’de yeşil kurt Helicoverpa armigera (Lepidoptera: Noctuidae) popülasyonun genetik çeşitliliği

Öz

Yeşil Kurt, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) dünyada tarımsal alanlarda yaygın olarak bilinen polifag bir zararlıdır. Domates üretiminde kalite ve ürünlerde önemli zararlara sebep olup, Türkiye’ de 1913 yılından beri bilinmektedir. Çalışmanın amacı, yeşil kurdun mitekondrial COI genindeki genetik çeşitliliği ve filogenetik benzerliğinin belirlenmesidir. 2017 ve 2018 yıllarında yeşil kurt ile bulaşık domatesler Çanakkale (14 alandan), Balıkesir (1 alan) ve Bursa (1 alandan) toplandı. Her örnekleme alanın temsil eden larvalar (n=3) kullanılarak genomik DNA izolasyonu yapıldı. COI geninin bir kısmı (536 bp) çoğaltılarak PCR ürünleri direkt olarak dizilimleri belirlendi. DnaSP ile haploit sayıları ve mutasyobları belirlendi. Populasyonlar arası genetik akrabalık düzeyleri UPGMA metodu ile MEGA X programında belirlendi. Sonuç olarak, test edilen 45 örnekte13 haplotip ve 13 mutasyon belirlendi. Buna ilave olarak yeşil kurtta 9 özel haplotip bulundu. Analizler sonucunda farklı cografik alanlardan elde edilen yeşil kurt popülasyonları 2 ayrı grupta yer aldı. İlk grup 12 haplotip (Çanakkale ve Balıkesir popülasyonu) ve diğer grupta ise sadec 1 haplotip (Bursa popülasyonu) yer aldı. Bu çalışma, Türkiye’de yeşil kurdun moleküler karekterizasyonu ve genetik çeşitliliğinin belirlenmesi için ilk kez yapılmıştır.

Anahtar Kelimeler

Helicoverpa armigera,, Cotton Bollworm,, Genetic diveristy,, mtDNA,, COI gene

Proje Numarası

FYL-2018/2466

Kaynakça

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