Green Synthesis of Gold Nanoparticles Using Aqueous Extract of Asphodelus Aestivus, Coating with Chitosan Biopolymer and Cytotoxicity Studies
Abstract
The green synthesis of gold nanoparticles (Au-NPs) was carried out by pouring the aqueous extract of East Anatolian origin Asphodelus aestivus plant onto aqueous gold metal ions and reducing them via single-step one- pot method. The absorption peak of the synthesized nanoparticles gave a maximum at 575 nm. All the X-ray diffraction peaks at 2θ = 38.25 , 44.46 , 64.64 and 77.20 that index to (111), (200), (220), and (311) planes verify the successful synthesis of Au-NPs. Mostly spherical shape particles showed a homogeneous distribution with size range 20±5 nm are measured using TEM. From the FTIR spectrum, the peaks are seems to be related to phenolic compounds, flavonoids, benzophenones, terpenoids and anthocyanins which assume that they could act as the reducing agents. The plant extraction, one-pot, single-step method used is environmentally safe without the role of synthetic materials which is highly potential in mild and green synthesis applications. The Au-NPs were coated with chitosan biopolymer in aquatic solution medium and verified by SEM. Then, cytotoxic investigations of the biosynthesized Au-NPs were carried out by HUVEC cells. Au-NPs were showed toxic effects on cell culture, even if in a small amount. However, chitosan biopolymer coating increased cell viability.
Keywords: Gold nanoparticle, Biosynthesis, Asphodelus aestivus, Biopolymer coating, Cytotoxicity
Keywords: Gold nanoparticle, Biosynthesis, Asphodelus aestivus, Biopolymer coating, Cytotoxicity
Keywords
Gold nanoparticle, Biosynthesis, Asphodelus aestivus, Biopolymer coating, Cytotoxicity
Project Number
YLMUB019-03
References
- [1] Yilmaz E., Ates M., Sahilli Y.Ç., Synthesizing, chitosan coating and detecting the nanotoxic effect of the lead selenide (PbSe) quantum dots, Digest Journal of Nanomaterials and Biostructures, 13(4) (2018) 1173-1182.
- [2] Rao K.J., Paria S., Aegle marmelos Leaf Extract and Plant Surfactants Mediated Green Synthesis of Au and Ag Nanoparticles by Optimizing Process Parameters Using Taguchi Method, ACS Sustainable Chemistry and Engineering, 3(3) (2015) 483–491.
- [3] Wu Y., Ali M.R.K., Chen K., Fang N., El-Sayed M.A., Gold nanoparticles in biological optical imaging, Nano Today, 24 (2019) 120-140.
- [4] Male D., Gromnicova R., McQuaid C., In: Al-Jamal K.T. (Eds). Gold Nanoparticles for Imaging and Drug Transport to the CNS, International Review of Neurobiology. Massachusetts: Academic Press (130) (2016) 155-198.
- [5] Yong K.T., Swihart M.T., Ding H., Prasad P.N., Preparation of Gold Nanoparticles and their Applications in Anisotropic Nanoparticle Synthesis and Bioimaging, Plasmonics, 4(2) (2009) 79–93.
- [6] Sanket S., Das S.K., Role of Enzymes in Synthesis of Nanoparticles. In: Thatoi H., Mohapatra S., Das S.K. (Eds). Bioprospecting of Enzymes in Industry, Healthcare and Sustainable Environment. Berlin: Springer, (2021) 139-153.
- [7] Guilger-Casagrande M., de Lima R., Synthesis of Silver Nanoparticles Mediated by Fungi: A Review, Frontiers in bioengineering and biotechnology, 7 (2019) Article: 287.
- [8] Khanna P., Kaur A., Goyal D., Algae-based metallic nanoparticles: Synthesis, characterization and applications, Journal of Microbiological Methods, 163 (2019) 105656.
- [9] Kumar, S.V., Rajeshkumar, S., Plant-Based Synthesis of Nanoparticles and Their Impact. In: Tripathi D.G., Ahmad P., Sharma S., Chauhan D.K., Dubey N.K., (Eds). Nanomaterials in Plants, Algae, and Microorganisms. Massachusetts: Academic Press, 1 (2018) 33-57.
- [10] Mittal A.K., Chisti Y., Banerjee U.C., Synthesis of Metallic Nanoparticles Using Plant Extracts, Biotechnology Advances, 31/2 (2013) 346-356.