Investigation of In Vitro Effect and Molecular Docking of Aluminum on Glucose-6-Phosphate Dehydrogenase Activity

Dilara Ülger Özbek; Deniz Kuruçay

doi:10.17776/csj.1088724

EN

Investigation of In Vitro Effect and Molecular Docking of Aluminum on Glucose-6-Phosphate Dehydrogenase Activity

Abstract

Aim: Aluminum is one of the elements that is widely used in many sectors and is the most abundant element in nature. The harm of aluminum, which was thought to be harmless until recently and is actively used in daily life, is open to discussion. In this study, it was aimed to investigate the effect of Aluminum Sulphate [Al2(SO4)3] on Glucose-6-Phosphate Dehydrogenase (G6PDH) activity, which is a key enzyme that catalyzes the first step of the pentose phosphate pathway (PFP). In addition, enzyme activity are detailed with molecular docking studies. For the purpose of examining in vitro effect of Aluminium on G6PDH, 4 different concentration of substrate (D-glucose-6-P) (01.M, 0.08M, 0.05M, 0.03M) prepared and 10mM, 30mM Al2(SO4)3 was added G6PDH envoriment. G6PDH activity was measured by spectrofotometrically. Molecular docking studies were performed with DockingServer and HEX 8.0.0 programs. With the data obtained, the Vmax of G6PDH was calculated as 3.33 and Km=0.0323. When 10 mM and 30mM Al2(SO4)3 were added to the reaction environment, it was observed that there was a decrease in enzyme activity by 24.92% and 57.06%, respectively. It was observed that the increase in Al2(SO4)3 concentration was an uncompetitive inhibition due to a significant decrease in both Km and Vmax values of the enzyme.

Keywords

Aluminium, Glucose 6-Phosphat dehydrogenase, Uncompetitive inhibition, Enzyme.

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Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Dilara Ülger Özbek ^*
0000-0002-6834-020X
Türkiye

Deniz Kuruçay This is me
0000-0002-9255-3301
Türkiye

Publication Date

June 29, 2022

Submission Date

March 17, 2022

Acceptance Date

May 28, 2022

Published in Issue

Year 2022 Volume: 43 Number: 2

DOI

https://doi.org/10.17776/csj.1088724

IZ

https://izlik.org/JA27BW84WZ

APA

Ülger Özbek, D., & Kuruçay, D. (2022). Investigation of In Vitro Effect and Molecular Docking of Aluminum on Glucose-6-Phosphate Dehydrogenase Activity. Cumhuriyet Science Journal, 43(2), 232-237. https://doi.org/10.17776/csj.1088724

AMA

1.Ülger Özbek D, Kuruçay D. Investigation of In Vitro Effect and Molecular Docking of Aluminum on Glucose-6-Phosphate Dehydrogenase Activity. CSJ. 2022;43(2):232-237. doi:10.17776/csj.1088724

Chicago

Ülger Özbek, Dilara, and Deniz Kuruçay. 2022. “Investigation of In Vitro Effect and Molecular Docking of Aluminum on Glucose-6-Phosphate Dehydrogenase Activity”. Cumhuriyet Science Journal 43 (2): 232-37. https://doi.org/10.17776/csj.1088724.

EndNote

Ülger Özbek D, Kuruçay D (June 1, 2022) Investigation of In Vitro Effect and Molecular Docking of Aluminum on Glucose-6-Phosphate Dehydrogenase Activity. Cumhuriyet Science Journal 43 2 232–237.

IEEE

[1]D. Ülger Özbek and D. Kuruçay, “Investigation of In Vitro Effect and Molecular Docking of Aluminum on Glucose-6-Phosphate Dehydrogenase Activity”, CSJ, vol. 43, no. 2, pp. 232–237, June 2022, doi: 10.17776/csj.1088724.

ISNAD

Ülger Özbek, Dilara - Kuruçay, Deniz. “Investigation of In Vitro Effect and Molecular Docking of Aluminum on Glucose-6-Phosphate Dehydrogenase Activity”. Cumhuriyet Science Journal 43/2 (June 1, 2022): 232-237. https://doi.org/10.17776/csj.1088724.

JAMA

1.Ülger Özbek D, Kuruçay D. Investigation of In Vitro Effect and Molecular Docking of Aluminum on Glucose-6-Phosphate Dehydrogenase Activity. CSJ. 2022;43:232–237.

MLA

Ülger Özbek, Dilara, and Deniz Kuruçay. “Investigation of In Vitro Effect and Molecular Docking of Aluminum on Glucose-6-Phosphate Dehydrogenase Activity”. Cumhuriyet Science Journal, vol. 43, no. 2, June 2022, pp. 232-7, doi:10.17776/csj.1088724.

Vancouver

1.Dilara Ülger Özbek, Deniz Kuruçay. Investigation of In Vitro Effect and Molecular Docking of Aluminum on Glucose-6-Phosphate Dehydrogenase Activity. CSJ. 2022 Jun. 1;43(2):232-7. doi:10.17776/csj.1088724

As of 2026, Cumhuriyet Science Journal will be published in six issues per year, released in February, April, June, August, October, and December

Abstract

Investigation of In Vitro Effect and Molecular Docking of Aluminum on Glucose-6-Phosphate Dehydrogenase Activity

Abstract

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References

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