Antifungal and Antibiofilm Activities of Some Essential Oils Against Candida spp

Bydaa Atron; Hanaou Ahamada; Ayşe Hümeyra Taşkın Kafa; Cem Çelik; Mürşit Hasbek

doi:10.17776/csj.1102028

EN

Antifungal and Antibiofilm Activities of Some Essential Oils Against Candida spp

Abstract

Candida species are commonly encountered strains associated with a wide range of infections. Unlike bacterial pathogens, fungal pathogens treatment is difficult and the development of resistance has been increasing at an alarming rate. In this study, the antifungal and antibiofilm effect of thyme oil, rosemary oil, mint oil, citronella oil, was tested on Candida albicans, Candida tropicalis, Candida kefyr, Candida glabrata, Candida parapsilosis isolated from clinical samples. The agar disc diffusion method was employed to determine the antifungal effect of the essential oils, and the inhibition of biofilm formation was assessed using microtiter biofilm inhibition assay. The results indicated that all the essential oils inhibited Candida strains and their biofilm in varying degrees. The highest antifungal activity in all isolates was observed in the thyme oil (>50mm), while rosemary oil showed the highest antibiofilm effect (>77%) in all tested strains. These findings led us to assume that the active components found in essential oils might be potential antifungal agents, adding to the repertoire of therapeutic options for the treatment of candidiasis.

Keywords

Antibiofilm, Antifungal, Citronella oil, Mint oil, Thyme oil.

References

[1] Bongomin F., Gago S., Oladele R O., Denning DW., Global and multi-national prevalence of fungal diseases-estimate precision, Journal of Fungi, 3 (4) (2017) 57-69.
[2] Cleveland A., Harrison L., Farley M., Hollick R., Stein B., Chiller T., Declining Incidence of Candidemia and the Shifting Epidemiology of Candida Resistance in Two US Metropolitan Areas, 2008–2013: Results from Population-Based Surveillance., PLOS ONE, 10(3) (2015) e0120452..
[3] Nobile C., Johnson A., Candida albicans Biofilms and Human Disease, Annual Review Of Microbiology, 69(1) (2015) 71-92.
[4] Calogiuri G., Garvey L. H., Nettis E., Romita P., Di Leo E., Caruso R., Foti C., Skin allergy to azole antifungal agents for systemic use: A review of the literature, Recent Patents On Inflammation & Allergy Drug Discovery, 13 (2) (2019) 144–157.
[5] Di Bonaventura G., Spedicato I., Picciani C., D'Antonio D., Piccolomini R., In vitro pharmacodynamic characteristics of amphotericin B, caspofungin, fluconazole, and voriconazole against bloodstream isolates of infrequent Candida species from patients with hematologic malignancies, Antimicrobial Agents and Chemotherapy, 48(11) (2004) 4453–4456..
[6] Silva S., Rodrigues C., Araújo D., Rodrigues M., Henriques M., Candida Species Biofilms’ Antifungal Resistance, Journal Of Fungi, 3(1) (2017) 8-11
[7] Chandra J., Mukherjee, P., Candida Biofilms: Development, Architecture, and Resistance, Microbiology Spectrum, 3(4) (2015) 70-85.
[8] Nett J., Andes D., Contributions of the Biofilm Matrix to Candida Pathogenesis. Journal Of Fungi, 6(1) (2020) 21-33.
[9] Rajkowska K., Nowicka-Krawczyk P., Kunicka-Styczyńska A., Effect of Clove and Thyme Essential Oils on Candida Biofilm Formation and the Oil Distribution in Yeast Cells, Molecules, 24(10) (2019) 42-54.
[10] Ali B., Al-Wabel N., Shams S., Ahamad A., Khan S., Anwar F., Essential oils used in aromatherapy: A systemic review, Asian Pacific Journal of Tropical Biomedicine, 5(8) (2015) 601-611.

[11] Bauer, A. W., W. M. M. Kirby, J. C. Sherris, and M. Turck. Antibiotic susceptibility testing by a standardized single disk method, American Journal of Clinical Pathology, 36 (1966) 493-496.
[12] Raveau R., Fontaine J., Lounès-Hadj Sahraoui A. Essential Oils as Potential Alternative Biocontrol Products against Plant Pathogens and Weeds: A Review, Foods, 9(3) (2020) 349-365.
[13] Onsare, J., and Arora, D. Antibiofilm potential of flavonoids extracted from Moringa oleifera seed coat against Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans, Journal of Applied Microbiology, 118 (3) (2015) 313–325.
[14] Jayathilaka E., Rajapaksha D., Nikapitiya C., De Zoysa M., Whang I., Antimicrobial and Anti-Biofilm Peptide Octominin for Controlling Multidrug-Resistant Acinetobacter baumannii, International Journal Of Molecular Sciences, 22(10) (2021) 53-55.
[15] Bachmann S., VandeWalle K., Ramage G., Patterson T., Wickes B., Graybill J., Lopez-Ribot J., In vitro activity of caspofungin against Candida albicans biofilms, Antimicrobial Agents and Chemotherapy, 46 (2002) 3591-3596
[16] Chandra J., Mukherjee P., Leidich S., Faddoul F., Hoyer L., Douglas J., Ghannoum M., Antifungal resistance of candidal biofilms formed on denture acrylic in vitro, Journal of Dental Research, 80(1) (2001) 903-908
[17] Shuford J., Piper K., Steckelberg J., PatelIn R., vitro biofilm characterization and activity of antifungal agents alone and in combination against sessile and planktonic clinical Candida albicans isolates, Diagnostic Microbiology and Infectious Disease, 57(3) (2007) 277-279
[18] Chouhan S, Sharma K., Guleria S., Antimicrobial activity of some essential oils -present status and future perspectives, Medicines, 4(3) (2017) 58-71.
[19] Bona E., Cantamessa S., Pavan M., Novello G., Massa N., Rocchetti, A.,Sensitivity of Candida albicans to essential oils: are they an alternative to antifungal agents?, Journal Of Applied Microbiology, 121(6) (2016) 1530-1545.
[20] Kızıl S., Hasımı N., Tolan V., Kılınc E. Yuksel U., Mineral content, essential oil components and biological activity of two Mentha species (M. piperita L., M. spicata L, Turkish Journal Of Field Crops, 15(2) (2010) 148-153.
[21] Saharkhiz M., Motamedi M., Zomorodian K., Pakshir K., Miri R., Hemyari, K., Chemical Composition, Antifungal and Antibiofilm Activities of the Essential Oil of Mentha piperita, ISRN Pharmaceutics, 20(12) (2012) 1-6.
[22] Benzaid C., Belmadani A., Djeribi R., Rouabhia M., The effects of Mentha piperita essential oil on C. albicans growth, transition, biofilm formation, and the expression of the secreted aspartyl proteinases genes, Antibiotics, 8(1) (2019) 10.
[23] Samber, N., Khan, A., Varma, A., & Manzoor, N. Synergistic anti-candidal activity and mode of action of Mentha piperita essential oil and its major components, Pharmaceutical Biology, 53(10) (2015)1496–1504.
[24] Sharma R., Rao R., Kumar S., Mahant S., Khatkar S., Therapeutic Potential of Citronella Essential Oil: A Review, Current Drug Discovery Technologies, 16(4) (2019) 330–339.
[25] Nakahara K., Alzoreky N.S., Yoshihashi T., Nguyen T., Trakoontivakorn G. Chemical composition and antifungal activity of essential oil from Cymbopogon nardus (Citronella Grass), Japan International Research Center for Agricultural Sciences, 37 (2013) 249–52.
[26] Khan M. S. A., Ahmad I., Antibiofilm activity of certain phytocompounds and their synergy with fluconazole against Candida albicans biofilms, Journal of Antimicrobial Chemotherapy, 67(3) (2011) 618–621.
[27] González-Trujano M., Peña E., Martínez A., Moreno J., Guevara-Fefer, P., Déciga-Campos, M., López-Muñoz F., Evaluation of the antinociceptive effect of Rosmarinus officinalis L. using three different experimental models in rodents, Journal of Ethnopharmacology, 11(3) (2007) 476–482.
[28] Trindade, L.A., Cordeiro, L.V., de Figuerêdo Silva, D. et al. The antifungal and antibiofilm activity of Cymbopogon nardus essential oil and citronellal on clinical strains of Candida albicans, Braz J. Microbiol., 53(3) 1231-1240.
[29] Matsuzaki, Y. , Tsujisawa, T. , Nishihara, T. , Nakamura, M. and Kakinoki, Y. Antifungal activity of chemotype essential oils from rosemary against Candida albicans, Open Journal of Stomatology, 3(2) (2013) 176-182.
[30] Alves, M., Gonçalves, M., Zuzarte, M., Alves-Silva, J., Cavaleiro, C., Cruz, M., & Salgueiro, L. Unveiling the Antifungal Potential of Two Iberian Thyme Essential Oils: Effect on C. albicans Germ Tube and Preformed Biofilms, Frontiers In Pharmacology, 10(2) (2019) 146-158
[31] Rajkowska, K., Nowicka-Krawczyk, P., & Kunicka-Styczyńska, A. Effect of Clove and Thyme Essential Oils on Candida Biofilm Formation and the Oil Distribution in Yeast Cells, Molecules (Basel, Switzerland), 24(10) (2019) 154-165
[32] Meccatti V. M., Oliveira J. R. D., Figueira W., Lagareiro Netto A., Zamarioli,L. S., Marcucci, C., Camargo E., Carvalho A., Oliveira D., Rosmarinus officinalis L. (rosemary) extract has antibiofilm effect similar to the antifungal nystatin on Candida samples, Anais Da Academia Brasileira de Ciências, 93(2) (2021) e20190366.
[33] Ghasemi G., Alirezalu A., Ghosta Y., Jarrahi A., Safavi S. A., Abbas-Mohammadi M., Barba J., Munekata S., Domínguez R., Lorenzo M., Composition, Antifungal, Phytotoxic, and Insecticidal Activities of Thymus kotschyanus Essential Oil, Molecules, 25(5) (2020) 152-164.
[34] Nabavi M., Marchese A., Izadi M., Curti V., Daglia M., Nabavi F., Plants belonging to the genus Thymus as antibacterial agents: From farm to pharmaca, Food Chemistr, 173 (2015) 339–347.
[35] Rajkowska K., Nowicka-Krawczyk P., Kunicka-Styczyńska A., Effect of Clove and Thyme Essential Oils on Candida Biofilm Formation and the Oil Distribution in Yeast Cells, Molecules, 24(10) (2019) 1954.

Details

Primary Language

English

Subjects

Structural Biology, Pharmacology and Pharmaceutical Sciences

Journal Section

Research Article

Authors

Bydaa Atron ^*
0000-0002-7571-9277
Türkiye

Hanaou Ahamada
0000-0002-0000-2239
Türkiye

Ayşe Hümeyra Taşkın Kafa
0000-0002-7282-4928
Türkiye

Cem Çelik
0000-0002-7141-5874
Türkiye

Mürşit Hasbek
0000-0002-5217-8607
Türkiye

Publication Date

September 30, 2022

Submission Date

April 12, 2022

Acceptance Date

August 4, 2022

Published in Issue

Year 2022 Volume: 43 Number: 3

DOI

https://doi.org/10.17776/csj.1102028

IZ

https://izlik.org/JA87SJ74SL

APA

Atron, B., Ahamada, H., Taşkın Kafa, A. H., Çelik, C., & Hasbek, M. (2022). Antifungal and Antibiofilm Activities of Some Essential Oils Against Candida spp. Cumhuriyet Science Journal, 43(3), 404-408. https://doi.org/10.17776/csj.1102028

AMA

1.Atron B, Ahamada H, Taşkın Kafa AH, Çelik C, Hasbek M. Antifungal and Antibiofilm Activities of Some Essential Oils Against Candida spp. CSJ. 2022;43(3):404-408. doi:10.17776/csj.1102028

Chicago

Atron, Bydaa, Hanaou Ahamada, Ayşe Hümeyra Taşkın Kafa, Cem Çelik, and Mürşit Hasbek. 2022. “Antifungal and Antibiofilm Activities of Some Essential Oils Against Candida Spp”. Cumhuriyet Science Journal 43 (3): 404-8. https://doi.org/10.17776/csj.1102028.

EndNote

Atron B, Ahamada H, Taşkın Kafa AH, Çelik C, Hasbek M (September 1, 2022) Antifungal and Antibiofilm Activities of Some Essential Oils Against Candida spp. Cumhuriyet Science Journal 43 3 404–408.

IEEE

[1]B. Atron, H. Ahamada, A. H. Taşkın Kafa, C. Çelik, and M. Hasbek, “Antifungal and Antibiofilm Activities of Some Essential Oils Against Candida spp”, CSJ, vol. 43, no. 3, pp. 404–408, Sept. 2022, doi: 10.17776/csj.1102028.

ISNAD

Atron, Bydaa - Ahamada, Hanaou - Taşkın Kafa, Ayşe Hümeyra - Çelik, Cem - Hasbek, Mürşit. “Antifungal and Antibiofilm Activities of Some Essential Oils Against Candida Spp”. Cumhuriyet Science Journal 43/3 (September 1, 2022): 404-408. https://doi.org/10.17776/csj.1102028.

JAMA

1.Atron B, Ahamada H, Taşkın Kafa AH, Çelik C, Hasbek M. Antifungal and Antibiofilm Activities of Some Essential Oils Against Candida spp. CSJ. 2022;43:404–408.

MLA

Atron, Bydaa, et al. “Antifungal and Antibiofilm Activities of Some Essential Oils Against Candida Spp”. Cumhuriyet Science Journal, vol. 43, no. 3, Sept. 2022, pp. 404-8, doi:10.17776/csj.1102028.

Vancouver

1.Bydaa Atron, Hanaou Ahamada, Ayşe Hümeyra Taşkın Kafa, Cem Çelik, Mürşit Hasbek. Antifungal and Antibiofilm Activities of Some Essential Oils Against Candida spp. CSJ. 2022 Sep. 1;43(3):404-8. doi:10.17776/csj.1102028

Cited By

Evaluation of the Antifungal Activity of Clove and Cinnamon Essential Oils against Candida Albicans

UMYU Journal of Microbiology Research (UJMR)

https://doi.org/10.47430/ujmr.25103.017

As of 2026, Cumhuriyet Science Journal will be published in six issues per year, released in February, April, June, August, October, and December