Electrospun Poly(ϵ-caprolactone) Nanofibers Containing Pomegranate Peel Extract and Bioactive Glass as Potential Wound Dressings
Abstract
This study focuses on the effect of pomegranate peel extract (PPE) as a natural medicinal substance and 45S5 bioglass (BG) particles as a bioactive material on the microstructure, antioxidant properties, and fibroblast cell cytotoxicity of biocompatible poly(ε-caprolactone) (PCL) nanofiber scaffolds. The hybrid nanofibers were fabricated via the electrospinning technique. The microstructure of nanofiber scaffolds was characterized by using scanning electron microscopy (SEM). The results indicated that the incorporation of PPE and BG particles did not change the morphology of the fibrous structure of the PCL nanofiber scaffolds. The DPPH analysis was performed to determine the antioxidant properties of nanofiber scaffolds and demonstrated that the incorporation of PPE improves the antioxidant properties of scaffolds. Cell cytotoxicity studies using fibroblast L929 cells also showed that high cell viability values were observed for hybrid PPE and BG loaded PCL nanofiber scaffolds. The findings proved that the integration of PPE and BG particles into PCL nanofibers yielded favorable characteristics suitable for wound dressing purposes, involving improved antioxidant capacity.
Keywords
Electrospinning, Wound dressings, Polycaprolactone, Pomegranate peel extract, Bioactive glass
References
- [1] Dhivya S., Padma V.V., Santhini E., Wound dressings - A review, Biomed. 5 (4) (2015) 22.
- [2] Yang M., Yu S., Zhao P., Shi G., Guo Y., Xie L, Lyu G., Yu J., Fabrication of biologically inspired electrospun collagen/silk fibroin/bioactive glass composited nanofibrous to accelerate the treatment efficiency of wound repair, Int. Wound J. 20 (3) (2023) 687-698.
- [3] Sergi R., Bellucci D., Salvatori R., Cannillo V., Chitosan-based bioactive glass gauze: Microstructural properties, in vitro bioactivity, and biological tests, Materials (Basel), 13 (12) (2020) 2819.
- [4] Zhou J., Wang H., Zhao S., Zhou N., Li L., Huang W., Huang W., Wang D., Zhang C., In vivo and in vitro studies of borate based glass micro-fibers for dermal repairing, Mater. Sci. Eng. C. 60 (2016) 437-445.
- [5] Baino F., Bioactive glasses – When glass science and technology meet regenerative medicine, Ceram. Int. 44 (13) (2018) 14953-14966.
- [6] Bakar S.A.A, Ali A.M., Noor S.N.F.M., Hamid S.B.S., Azhar N.A., Mohamad N.M., Ahmad N.H., Combination of Goniothalamin and Sol-Gel-Derived Bioactive Glass 45S5 Enhances Growth Inhibitory Activity via Apoptosis Induction and Cell Cycle Arrest in Breast Cancer Cells MCF-7, Biomed Res. Int. 2022 (2022).
- [7] Nandhakumar M., Thangaian D.T., Sundaram S., Roy A., Subramanian B., An enduring in vitro wound healing phase recipient by bioactive glass-graphene oxide nanocomposites, Sci. Rep. 12 (1) (2022) 16162.
- [8] Deliormanlı A.M, Konyalı R., Bioactive glass/hydroxyapatite-containing electrospun poly(ε-caprolactone) composite nanofibers for bone tissue engineering, J. Aust. Ceram. Soc. 55 (2) (2019) 247-256.
- [9] Sergi R., Cannillo V., Boccaccini A.R., Liverani L., Incorporation of bioactive glasses containing Mg, Sr, and Zn in electrospun PCL fibers by using benign solvents, Appl. Sci. 10 (16) (2020) 5530.
- [10] Akturk A., Erol-Taygun M., Goller G., Küçükbayrak S., Optimization and characterization of poly(ℇ-caprolactone) nanofiber mats doped with bioactive glass and copper metal nanoparticles, Chem. Pap. 75 (11) (2021) 5929-5943.