Crocus Officinalis (L.) Extract on Human Colerectal Cancer Cell Line (HT-22): Investigation in Vitro
Abstract
Crocus sativus L. has been used as spice, food colouring and medicinal plant for thousands of years. In this study, antioxidant properties and cytotoxic effect of saffron extract were evaluated in HT-22 cell lines. Malignant and non-malignant cells (L929) were cultured in DMEM medium and incubated with different concentrations of saffron extract in different solvents (water, ethanol, ethyl-acetate, n-hexane). Cell viability was determined by MTT assay. Antioxidant parameters such as DPPH, FRAP, total polyphenol and flavonoid amounts were measured. The highest DPPH value was found as 15.90±0.12 µg/mL in ethyl acetate solvent. Total polyphenols were 89.06±0.02 mg GAE/g extract, flavonoids were 76.98±2.08 µg QE/g extract and FRAP was 67.93±0.98 mg/g extract in ethanol solvent. In colon cancer, IC50 value 94±1.37 µg/mL was found to be the highest value. This is thought to be due to the high antioxidant capacity in ethanol solvent. While antioxidant capacity was not found to be high, antiproliferation effect was found to be high. Saffron may also be considered as a promising chemotherapeutic agent in cancer treatment in the future.
Keywords
Antioxidant parameters, Crocus sativus L., HT-22 cell lines, Saffron
References
- [1] Smith-Warner S.A., Elmer P.J., Tharp T.M., Fosdick L., Randall B., Gross M., Wood J., Potter J.D., Increasing vegetable and fruit intake: randomized intervention and monitoring in an at-risk population, Cancer Epidemiol Biomar Prev., 9 (3) (2000) 307-317.
- [2] World Health Organization. 2018. Global Health Observatory. http: //www.who.int.- /data/gho Erişim tarihi: 21.06.2018
- [3] Doğruyol H., Additives in Foods and Their Harm; Childhood Hyperactivity, Current Pediatrics., 4 (2) (2006) 42-48.
- [4] Abdullaev F.I., Espinosa-Aguirre J.J., Biomedical properties of saffron and its potential use in cancer therapy and chemoprevention trials, Cancer Detection and Prevention., 28 (6) (2004) 426-432.
- [5] Khazdair M.R., Boskabady M.H., Hosseini M., Rezaee R., Tsatsakis A.M., The effects of Crocus sativus (saffron) and its constituents on nervous system: A review, Avicenna J Phytomed., 5 (5) (2015) 376-391.
- [6] Behzad S., Razavi M., Mahajeri M., The effect of mineral nutrients (N.P.K.) on saffron production, Acta Hort., 306 (1992) 426-430.
- [7] Melnyk J.P., Wang S., Marcone M.F., Chemical and biological properties of the world's most expensive spice: Saffron, Food Research International., 43 (8) (2010) 1981– 1989.
- [8] Bolhassani A., Khavari A., Bathaie S.Z., Saffron and natural carotenoids: Biochemical activities and anti-tumor effects, Biochimica et Biophysica Acta (BBA)-Reviews on Cancer., 1845 (1 )(2014) 20-30.
- [9] Rios, J. L., Recio, M. C., Giner, R. M. ve Manez, S. (1996). An Update Review of Saffron and İts Active Constituents. Phytotherapy Research, 10(3), 189-193.
- [10] Zararsız H. F., Gastronomi Ve Mutfak Sanatları Anabilim Dalı, master thesis, karabük of university, Lisansüstü Eğitim of institue, 2023.