Chlorogenic Acid: HPLC Quantification and In Vitro Assessment of Proliferative and Migration Effects on Human Dermal Fibroblast Cells

Abdulbaki Akpınar; Fatma Demirkaya Miloğlu; Gülşah Gündoğdu; Leyla Güven; Burak Bayrak; Yücel Kadıoğlu

doi:10.17776/csj.1440382

EN

Chlorogenic Acid: HPLC Quantification and In Vitro Assessment of Proliferative and Migration Effects on Human Dermal Fibroblast Cells

Abstract

Chlorogenic acid (CA) exhibits diverse biological activities, including antioxidant and antiinflammatory effects. This research aims to develop, optimize, and validate an HPLC method to quantify CA in methanol and investigate its in vitro proliferative and cell migration effects on human-dermal-fibroblast (HDF) cell lines in a dose-dependent manner. The HPLC experimental conditions were optimized using the central composite design (CCD) method for determining CA. Chromatographic separation occurred at a wavelength of 330 nm. Under the optimized conditions, the method exhibited linearity across a concentration range of 0.1-100 µg/mL, demonstrating sensitivity (LOQ:0.1µg/mL), precision (RSD%≤3.32), and accuracy (RE%≤4.05). To evaluate the in vitro proliferative and cell migration effects on HDFs, we employed the XTT cell proliferation assay and TAS-TOS commercial kits. The XTT assay revealed that CA displayed a proliferative effect within the concentration range of 75-250 µM (P <0.01), and at a concentration of 125 µM, TAS levels increased significantly (P<0.05). The scratch assay demonstrated that HDF cell migration increased at 12 h, with substantial closure of the wound area at 24 h when treated with CA concentrations between 75-125 µM. The results demonstrate that pure chlorogenic acid extracted from plants exhibits dose-dependent effects on cell proliferation, antioxidant, and cell migration

Keywords

Chlorogenic acid, HPLC, Experimental design, Central composite design

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Details

Primary Language

English

Subjects

Quality Assurance, Chemometrics, Traceability and Metrological Chemistry

Journal Section

Research Article

Authors

Abdulbaki Akpınar ^*
0000-0001-7318-6529
Türkiye

Fatma Demirkaya Miloğlu
0000-0001-5729-7181
Türkiye

Gülşah Gündoğdu
0000-0002-9924-5176
Türkiye

Leyla Güven
0000-0002-3189-6415
Türkiye

Burak Bayrak
0000-0001-6550-6916
Türkiye

Yücel Kadıoğlu
0000-0001-6590-7306
Türkiye

Publication Date

June 30, 2024

Submission Date

February 20, 2024

Acceptance Date

June 21, 2024

Published in Issue

Year 2024 Volume: 45 Number: 2

DOI

https://doi.org/10.17776/csj.1440382

IZ

https://izlik.org/JA42AC27PD

APA

Akpınar, A., Demirkaya Miloğlu, F., Gündoğdu, G., Güven, L., Bayrak, B., & Kadıoğlu, Y. (2024). Chlorogenic Acid: HPLC Quantification and In Vitro Assessment of Proliferative and Migration Effects on Human Dermal Fibroblast Cells. Cumhuriyet Science Journal, 45(2), 299-308. https://doi.org/10.17776/csj.1440382

AMA

1.Akpınar A, Demirkaya Miloğlu F, Gündoğdu G, Güven L, Bayrak B, Kadıoğlu Y. Chlorogenic Acid: HPLC Quantification and In Vitro Assessment of Proliferative and Migration Effects on Human Dermal Fibroblast Cells. CSJ. 2024;45(2):299-308. doi:10.17776/csj.1440382

Chicago

Akpınar, Abdulbaki, Fatma Demirkaya Miloğlu, Gülşah Gündoğdu, Leyla Güven, Burak Bayrak, and Yücel Kadıoğlu. 2024. “Chlorogenic Acid: HPLC Quantification and In Vitro Assessment of Proliferative and Migration Effects on Human Dermal Fibroblast Cells”. Cumhuriyet Science Journal 45 (2): 299-308. https://doi.org/10.17776/csj.1440382.

EndNote

Akpınar A, Demirkaya Miloğlu F, Gündoğdu G, Güven L, Bayrak B, Kadıoğlu Y (June 1, 2024) Chlorogenic Acid: HPLC Quantification and In Vitro Assessment of Proliferative and Migration Effects on Human Dermal Fibroblast Cells. Cumhuriyet Science Journal 45 2 299–308.

IEEE

[1]A. Akpınar, F. Demirkaya Miloğlu, G. Gündoğdu, L. Güven, B. Bayrak, and Y. Kadıoğlu, “Chlorogenic Acid: HPLC Quantification and In Vitro Assessment of Proliferative and Migration Effects on Human Dermal Fibroblast Cells”, CSJ, vol. 45, no. 2, pp. 299–308, June 2024, doi: 10.17776/csj.1440382.

ISNAD

Akpınar, Abdulbaki - Demirkaya Miloğlu, Fatma - Gündoğdu, Gülşah - Güven, Leyla - Bayrak, Burak - Kadıoğlu, Yücel. “Chlorogenic Acid: HPLC Quantification and In Vitro Assessment of Proliferative and Migration Effects on Human Dermal Fibroblast Cells”. Cumhuriyet Science Journal 45/2 (June 1, 2024): 299-308. https://doi.org/10.17776/csj.1440382.

JAMA

1.Akpınar A, Demirkaya Miloğlu F, Gündoğdu G, Güven L, Bayrak B, Kadıoğlu Y. Chlorogenic Acid: HPLC Quantification and In Vitro Assessment of Proliferative and Migration Effects on Human Dermal Fibroblast Cells. CSJ. 2024;45:299–308.

MLA

Akpınar, Abdulbaki, et al. “Chlorogenic Acid: HPLC Quantification and In Vitro Assessment of Proliferative and Migration Effects on Human Dermal Fibroblast Cells”. Cumhuriyet Science Journal, vol. 45, no. 2, June 2024, pp. 299-08, doi:10.17776/csj.1440382.

Vancouver

1.Abdulbaki Akpınar, Fatma Demirkaya Miloğlu, Gülşah Gündoğdu, Leyla Güven, Burak Bayrak, Yücel Kadıoğlu. Chlorogenic Acid: HPLC Quantification and In Vitro Assessment of Proliferative and Migration Effects on Human Dermal Fibroblast Cells. CSJ. 2024 Jun. 1;45(2):299-308. doi:10.17776/csj.1440382

Cited By

Microencapsulation of Black Carrot Pomace Bioactive Compounds: Artificial Neural Network Modeling of Cytotoxicity on L929 Fibroblast Cells

Gels

https://doi.org/10.3390/gels12010053

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