Ir-Pd/CNT Modifiye Elektrotlarla Triptofan Tespiti için Hassas Bir Elektrokimyasal Sensörün Geliştirilmesi
Abstract
In this study, a novel carbon nanotube-supported bimetallic Iridium-Palladium (Ir-Pd) catalyst, denoted as Ir(50%)-Pd(50%)/CNT, was successfully synthesized using the sodium borohydride reduction method for the electrochemical detection of L-Tryptophan (L-Trp). The catalyst was characterized using Elemental Dispersion X-ray (EDX-SEM) and X-ray Diffraction (XRD) techniques, confirming the effective dispersion of Ir and Pd nanoparticles on the carbon nanotube surface. Electrochemical performance was evaluated using Cyclic Voltammetry (CV), Differential Pulse Voltammetry (DPV), and Electrochemical Impedance Spectroscopy (EIS) to optimize the sensor's detection capabilities. The resulting Ir-Pd/CNT/GCE sensor demonstrated a linear response over the concentration range of 100-1000 µM, and an exceptional limit of detection (LOD) of 0.02 µM at a signal-to-noise ratio (S/N) of 3. These findings indicate the potential of the Ir-Pd/CNT/GCE sensor for highly sensitive L-Trp detection in various applications, including biomedical, environmental, and food monitoring. This study emphasizes the promising role of bimetallic catalysts in enhancing the performance of electrochemical sensors for amino acid detection.
Keywords
References
- Martins AC, Gloria MB., Changes on the levels of serotonin precursors–tryptophan and 5-hydroxytryptophan–during roasting of Arabica and Robusta coffee, Food Chemistry, 118(3) (2010) 529-33.
- Reynolds DM., Rapid and direct determination of tryptophan in water using synchronous fluorescence spectroscopy, Water Research, 37(13) (2003) 3055-60.
- Li, H., Li, F., Han, C., Cui, Z., Xie, G., Zhang, A., Highly sensitive and selective tryptophan colorimetric sensor based on 4, 4-bipyridine-functionalized silver nanoparticles, Sensors and Actuators B: Chemical, 145(1) (2010) 194-199.
- Lian, W., Ma, D. J., Xu, X. U., Chen, Y., & Wu, Y. L., Rapid high‐performance liquid chromatography method for determination of tryptophan in gastric juice, Journal of Digestive Diseases, 13(2) (2012) 100-106.
- Shahrokhian, S., Fotouhi, L., Carbon paste electrode incorporating multi-walled carbon nanotube/cobalt salophen for sensitive voltammetric determination of tryptophan, Sensors and Actuators B: Chemical, 123(2) (2007) 942-949.
- Fan, Y., Liu, J. H., Lu, H. T., Zhang, Q., Electrochemistry and voltammetric determination of L-tryptophan and L-tyrosine using a glassy carbon electrode modified with a Nafion/TiO2-graphene composite film, Microchimica Acta., 173 (2011) 241-247.
- Güney, S., Yıldız, G., Determination of tryptophan using electrode modified with poly (9-aminoacridine) functionalized multi-walled carbon nanotubes, Electrochimica Acta., 57 (2011) 290-296.
- Deng, K. Q., Zhou, J. H., Li, X. F., Direct electrochemical reduction of graphene oxide and its application to determination of L-tryptophan and L-tyrosine, Colloids and Surfaces B: Biointerfaces, 101 (2013) 183-188.
- Kivrak, H., Selcuk, K., Er, O. F., Aktas, N., Nanostructured electrochemical cysteine sensor based on carbon nanotube supported Ru, Pd, and Pt catalysts, Materials Chemistry and Physics, 267 (2021) 124689.
- Imanzadeh, H., Sefid-Sefidehkhan, Y., Afshary, H., Afruz, A., Amiri, M., Nanomaterial-based electrochemical sensors for detection of amino acids, Journal of Pharmaceutical and Biomedical Analysis, 230 (2023) 115390.