Understanding of Protein Adhesion to PLGA Through Molecular Docking Analysis
Abstract
In tissue engineering, cellular adhesion is a multistep process which occurs between the cell and the specific molecules and proteins that are adsorbed from biological fluids to the material surface. Therefore, it is essential to understand the protein adsorption level and which protein can bind efficiently to the surface of the fabricated material. Although many studies in literature showing quantification of protein adsorption levels by chemical methods and some instrumental techniques, there is a notable gap in the literature regarding the application of molecular docking analysis to identify which proteins can bind effectively to material surfaces, together with the specific interactions and orientations involved. Towards this goal, the present study aimed to evaluate the interactions between FDA approved biomaterial poly(lactic-co-glycolic acid) (PLGA) surface and specific proteins which are commonly found in cell culture medium for in vitro studies, and integrins which are associated with cellular adhesion. The results showed that PLGA exhibited strong binding affinity to both BSA and integrin β2, while less stable bindings were performed by hemoglobin and 2-macroglobulin proteins. These results indicate that how molecular recognition via docking process is important for fabricating biomaterials in tissue engineering applications.
Keywords
Molecular docking, PLGA, Cellular adhesion, Serum proteins
Project Number
The Scientific and Technological Research Council of Turkey (grant no: 217M952).
Ethical Statement
Not applicable
Thanks
The authors would like to thank the Scientific and Technological Research Council of Turkey (TÜBİTAK, Grant No: 217M952) for its partial financial support.
References
- [1] Berman D.I., Alfimov A.V., Zhigulskaya Z.A., Leirikh A.N., Overwintering and cold-hardiness of ants in the Northeast of Asia, Sofia-Moscow: Pensoft, (2010) 294.
- [2] Berman D.I., Leirikh A.N., Bessolitzina E.P., Three strategies of cold tolerance in click beetles (Coleoptera, Elateridae), Doklady Biological Sciences, 450 (2013) 168–172.
- [3] Berman D.I., Leirikh A.N., Bessolitzina E.P., Biotopical distribution and resistance to cold of click beetles (Coleoptera, Elateridae) in the Upper Kolyma basin, Euroasian Entomological Journal, (2017) 129–140.
- [4] Duman J.G., The role of macromolecular antifreeze in the darkling beetle, Meracantha contracta, Journal of Comparative Physiology, (1977) 279–286.
- [5] Duman J.G., Bennett V., Sformo T., Hochstrasser R., Barnes B.M., Antifreeze proteins in Alaskan insects and spiders, Journal of Insect Physiology, 50 (2004) 259–266.
- [6] Duman J.G., Animal ice-binding (antifreeze) proteins and glycolipids: an over view with emphasis on physiological function, Journal of Experimental Biology, 218 (2015) 1846-1855.
- [7] Storey J.M., Storey K.B., Cold hardiness and freeze tolerance, Functional Metabolism: Regulation and Adaptation, (2004).
- [8] Richard E., Lee R.E. Jr., Insect Cold-Hardiness: To Freeze or Not to Freeze, BioScience, 39 (1989) 308-313.
- [9] Denlinger D.L., Lee R.E. Jr., Low Temperature Biology of Insects, Cambridge University Press, (2010).
- [10] Białkowska A., Majewska E., Olczak A., Twarda-Clapa A., Ice Binding Proteins: Diverse Biological Roles and Applications in Different Types of Industry, Biomolecules, 10 (2020) 274.