A Study on the Interaction Between DNA and 6-Mercaptopurine Under Acidic and Basic Conditions
Abstract
In this study, the interaction between double-stranded DNA (fs dsDNA) obtained from fish sperm and the anticancer drug 6-mercaptopurine (6-MP) was investigated using the square wave voltammetry (SWV) method under both acidic (pH 4.8) and basic (pH 7.4) conditions. Within the scope of the study, the optimal immobilization times and concentrations of fs dsDNA and 6-MP were determined, and the effects of different pH environments on this interaction were evaluated. Based on the measurements, fs dsDNA yielded the highest signal at 30 minutes and 30 µg/mL in basic medium, and at 20 minutes and 30 µg/mL in acidic medium. For 6-MP, the optimal interaction time was found to be 5 minutes and the optimal concentration 6 µg/mL in both environments. The obtained calibration curves showed high accuracy (R² > 0.998), and low detection limits were achieved. This study demonstrates that DNA-drug interactions can be determined precisely, selectively, and rapidly through electrochemical methods, contributing to biosensor-based diagnostics and pharmaceutical analysis.
Keywords
6-Mercaptopurine, fs dsDNA, Square Wave Voltammetry, DNA-Drug Interaction, Biosensor.
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