Antiproliferative Effects, Brdu Assay and Antioxidant Activities of Origanum sipyleum L. Extraction on Breast Cancer Cell Lines: Investigation in Vitro

Gamze Tüzün; Ceylan Hepokur; Armagan Akkuş; Mohammad Omar Faruque; Sema Mısır

doi:10.17776/csj.1778624

Antiproliferative Effects, Brdu Assay and Antioxidant Activities of Origanum sipyleum L. Extraction on Breast Cancer Cell Lines: Investigation in Vitro

Abstract

Origanum sipyleum L. is commonly used for the treatment of cough, pain, cancer, and infectious diseases. In this study, the antioxidant and antiproliferative properties of this endemic species Origanum sipyleum L. (purple coral) were investigated. Quantitative analysis of the components of Origanum sipyleum L. was performed by HPLC. The antioxidant effects of Origanum sipyleum L. ethanol extract was examined. Among the antioxidant effects, Total Polyphenolic Content Determination, Total Flavonoid Content Determination, Plasma Ferric Reducing Ability Test and Radical Scavenging Activity (DPPH) Determination were examined. The cytotoxic effect was determined using the XTT assay against MCF-7 (human breast adenocarcinoma cell line), MDA-MB-231 (human breast cancer cell line), and L929 (mouse fibroblast cell line) cells. Calculated IC₅₀ values of Origanium Sipyleum L. were 205±6.86 against MCF-7 cells for 24 h, 147±4.23 for 48 h, 197±4.15 for 24 h and 96± 3.46 against MDA-MB-231 cells, and 415±1.67 for 24 h 221±2.13 for 48 h, against L929, respectively. Antiproliferative properties of Origanium sipyleum L. was assessed by flow cytometry. MDA-MB-231 cell lines were used to determine cell proliferation and BrdU assay was analyzed spectrophotometrically. Results postulated that Origanum sipyleum L. had a high antioxidant and antiproliferative effects.

Keywords

Antioxidant effect, Antiproliferative effects, BrDU assay, Origanum sipyleum L.

Supporting Institution

Sivas Cumhuriyet University

Project Number

CUBAP project no. ECZ-059

Ethical Statement

No

Thanks

The Authors thanks to Dr. Mehmet ÇİÇEK for collected plant.

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Details

Primary Language

English

Subjects

Cancer Biology

Journal Section

Research Article

Authors

Gamze Tüzün
0000-0002-5876-2046
Türkiye

Ceylan Hepokur ^*
0000-0001-6397-1291
Türkiye

Armagan Akkuş
0000-0002-8374-7892
Türkiye

Mohammad Omar Faruque
0000-0002-4684-5004
Bangladesh

Sema Mısır
0000-0002-5253-0420
Türkiye

Publication Date

February 27, 2026

Submission Date

September 5, 2025

Acceptance Date

December 30, 2025

Published in Issue

Year 2026 Volume: 47 Number: 1

DOI

https://doi.org/10.17776/csj.1778624

IZ

https://izlik.org/JA29ST36KH

APA

Tüzün, G., Hepokur, C., Akkuş, A., Faruque, M. O., & Mısır, S. (2026). Antiproliferative Effects, Brdu Assay and Antioxidant Activities of Origanum sipyleum L. Extraction on Breast Cancer Cell Lines: Investigation in Vitro. Cumhuriyet Science Journal, 47(1), 1-8. https://doi.org/10.17776/csj.1778624

AMA

1.Tüzün G, Hepokur C, Akkuş A, Faruque MO, Mısır S. Antiproliferative Effects, Brdu Assay and Antioxidant Activities of Origanum sipyleum L. Extraction on Breast Cancer Cell Lines: Investigation in Vitro. CSJ. 2026;47(1):1-8. doi:10.17776/csj.1778624

Chicago

Tüzün, Gamze, Ceylan Hepokur, Armagan Akkuş, Mohammad Omar Faruque, and Sema Mısır. 2026. “Antiproliferative Effects, Brdu Assay and Antioxidant Activities of Origanum Sipyleum L. Extraction on Breast Cancer Cell Lines: Investigation in Vitro”. Cumhuriyet Science Journal 47 (1): 1-8. https://doi.org/10.17776/csj.1778624.

EndNote

Tüzün G, Hepokur C, Akkuş A, Faruque MO, Mısır S (February 1, 2026) Antiproliferative Effects, Brdu Assay and Antioxidant Activities of Origanum sipyleum L. Extraction on Breast Cancer Cell Lines: Investigation in Vitro. Cumhuriyet Science Journal 47 1 1–8.

IEEE

[1]G. Tüzün, C. Hepokur, A. Akkuş, M. O. Faruque, and S. Mısır, “Antiproliferative Effects, Brdu Assay and Antioxidant Activities of Origanum sipyleum L. Extraction on Breast Cancer Cell Lines: Investigation in Vitro”, CSJ, vol. 47, no. 1, pp. 1–8, Feb. 2026, doi: 10.17776/csj.1778624.

ISNAD

Tüzün, Gamze - Hepokur, Ceylan - Akkuş, Armagan - Faruque, Mohammad Omar - Mısır, Sema. “Antiproliferative Effects, Brdu Assay and Antioxidant Activities of Origanum Sipyleum L. Extraction on Breast Cancer Cell Lines: Investigation in Vitro”. Cumhuriyet Science Journal 47/1 (February 1, 2026): 1-8. https://doi.org/10.17776/csj.1778624.

JAMA

1.Tüzün G, Hepokur C, Akkuş A, Faruque MO, Mısır S. Antiproliferative Effects, Brdu Assay and Antioxidant Activities of Origanum sipyleum L. Extraction on Breast Cancer Cell Lines: Investigation in Vitro. CSJ. 2026;47:1–8.

MLA

Tüzün, Gamze, et al. “Antiproliferative Effects, Brdu Assay and Antioxidant Activities of Origanum Sipyleum L. Extraction on Breast Cancer Cell Lines: Investigation in Vitro”. Cumhuriyet Science Journal, vol. 47, no. 1, Feb. 2026, pp. 1-8, doi:10.17776/csj.1778624.

Vancouver

1.Gamze Tüzün, Ceylan Hepokur, Armagan Akkuş, Mohammad Omar Faruque, Sema Mısır. Antiproliferative Effects, Brdu Assay and Antioxidant Activities of Origanum sipyleum L. Extraction on Breast Cancer Cell Lines: Investigation in Vitro. CSJ. 2026 Feb. 1;47(1):1-8. doi:10.17776/csj.1778624

Cited By

How distillation alters bioactivity: Comparative phytochemical and biological evaluation of three Lamiaceae species (Origanum sipyleum, Thymus sipyleus, and Sideritis phrygia) before and after distillation

Microchemical Journal

https://doi.org/10.1016/j.microc.2026.118739

As of 2026, Cumhuriyet Science Journal will be published in six issues per year, released in February, April, June, August, October, and December