The Hypoglycaemic Effect and Antioxidant and Anti-α-amylase Activity of the Hydroalcoholic Extract of Tamarix algeriensis G.Nicholson Tamaricaceae

Mohammed Tahar Ben Moussa; Chihab Eddine Zeggada; Abdallah Mouadh Rahmani; Hassina Harkat; Safa Chaira; Nawal Achachi

doi:10.17776/csj.1818332

The Hypoglycaemic Effect and Antioxidant and Anti-α-amylase Activity of the Hydroalcoholic Extract of Tamarix algeriensis G.Nicholson Tamaricaceae

Abstract

This study evaluated the hydroalcoholic extract of Tamarix algeriensis aerial parts' antihyperglycemic and antioxidant properties. In Batna, Algeria, the researchers gathered the plant's aerial parts, which they then macerated to create the extract. Numerous bioactive substances, such as flavonoids, saponins, polyphenols, steroids, terpenoids, tannins, and anthracene glycosides, were found during the initial phytochemical screening. Numerous techniques, such as ferric reducing power (FRAP), total antioxidant capacity (TAC), and DPPH free radical scavenging tests, were used to confirm the extract's strong antioxidant activity in vitro. Additionally, the extract demonstrated strong inhibitory activity against the enzyme α-amylase, which is essential for the breakdown of carbohydrates. Tamarix algeriensis extract significantly decreased blood glucose levels after a glucose load in in vivo tests on normoglycaemic mice, demonstrating a hypoglycaemic effect. These findings imply that Tamarix algeriensis hydroalcoholic extract may be a useful source of therapeutic compounds for the treatment of diabetes and oxidative stress.

Keywords

Antioxidant activity, Diabetes, Hypoglycemic activity, Tamarix algeriensis, α-amylase inhibition

Supporting Institution

faculty of medecine Annaba

Ethical Statement

IRB

Thanks

The writers acknowledge the Algerian Ministry of Higher Education and Scientific Research for their assistance inside the context of the PRFU project (approval n° B00L01UN052).

References

[1] Singh, N., Kesherwani, R., Tiwari, A. K., & Patel, D. K. (2016). A review on diabetes mellitus. The Pharma Innovation, 5(7), 36–40.
[2] Firdous, S. M., Irfan, Z., Giri, S., & Asaba, N. S. (2025). Unravelling the role of natural herbs for the management of diabetes mellitus. Diabetology & Metabolic Syndrome, 17(1), 12. https://doi.org/10.1186/s13098-025-01904-4
[3] Bánki, O., Roskov, Y., Döring, M., Ower, G., Hernández Robles, D. R., Plata Corredor, C. A., et al. (2025). Catalogue of Life: Annual Checklist 2025. Catalogue of Life.
[4] Bouabid, K., Lamchouri, F., Toufik, H., Boulfia, M., Senhaji, S., & Faouzi, M. E. A. (2019). In vivo anti-diabetic effect of aqueous and methanolic macerated extracts of Atractylis gummifera. Bangladesh Journal of Pharmacology, 14(2), 67–73. https://doi.org/10.3329/bjp.v14i2.38870
[5] Falleh, H., Ksouri, R., Chaieb, K., Karray-Bouraoui, N., Trabelsi, N., Boulaaba, M., & Abdelly, C. (2008). Phenolic composition of Cynara cardunculus L. organs, and their biological activities. Comptes Rendus Biologies, 331(5), 372–379. https://doi.org/10.1016/j.crvi.2008.02.008
[6] Wagner, H., & Bladt, S. (1996). Plant drug analysis: A thin layer chromatography atlas (2nd ed.). Springer. http://dx.doi.org/10.1007/978-3-642-00574-9
[7] da Silva Peixoto Sobrinho, T. J., de Sousa Araújo, T. A., de Almeida, C. F. C. B. R., & de Amorim, E. L. C. (2014). Chemical tools applied to ethnobotanical studies. In U. P. Albuquerque, L. V. F. C. da Cunha, R. F. P. de Lucena, & R. R. N. Alves (Eds.), Methods and techniques in ethnobiology and ethnoecology (pp. 255–274). Springer. https://doi.org/10.1007/978-1-4614-8636-7_16
[8] Hatami, T., Emami, S. A., Miraghaee, S. S., & Mojarrab, M. (2014). Total phenolic contents and antioxidant activities of different extracts and fractions from the aerial parts of Artemisia biennis Willd. Iranian Journal of Pharmaceutical Research, 13(2), 551–559. https://doi.org/10.22037/ijpr.2014.1518
[9] Matić, P., Sabljić, M., & Jakobek, L. (2017). Validation of spectrophotometric methods for the determination of total polyphenol and total flavonoid content. Journal of AOAC International, 100(6), 1795–1803. https://doi.org/10.5740/jaoacint.17-0066
[10] European Medicines Agency. (2022). Annual report 2021: The European Medicines Agency’s contribution to science, medicines and health in 2021. EMA.

[11] Bossu, C. M., Ferreira, E. C., Chaves, F. S., Menezes, E. A., & Nogueira, A. R. A. (2006). Flow injection system for hydrolysable tannin determination. Microchemical Journal, 84(1), 88–92. https://doi.org/10.1016/j.microc.2006.04.018
[12] Naima, R., Oumam, M., Hannache, H., Sesbou, A., Charrier, B., Pizzi, A., & Charrier-El Bouhtoury, F. (2015). Comparison of the impact of different extraction methods on polyphenols yields and tannins from Acacia mollissima barks. Industrial Crops and Products, 70, 245–252. https://doi.org/10.1016/j.indcrop.2015.03.016
[13] Scalbert, A., Monties, B., & Janin, G. (1989). Tannins in wood: Comparison of different estimation methods. Journal of Agricultural and Food Chemistry, 37(5), 1324–1329.
[14] Hartzfeld, P. W., Forkner, R., Hunter, M. D., & Hagerman, A. E. (2002). Determination of hydrolyzable tannins after reaction with potassium iodate. Journal of Agricultural and Food Chemistry, 50(7), 1785–1790. https://doi.org/10.1021/jf0111155
[15] Morales, G., & Paredes, A. (2014). Antioxidant activities of Lampaya medicinalis extracts and their main chemical constituents. BMC Complementary and Alternative Medicine, 14, 7. https://doi.org/10.1186/1472-6882-14-259
[16] Stefanello, S. T., Prestes, A. S., Ogunmoyole, T., Salman, S. M., Schwab, R. S., Brender, C. R., et al. (2013). Evaluation of in vitro antioxidant effect of new mono and diselenides. Toxicology in Vitro, 27(5), 1433–1439. https://doi.org/10.1016/j.tiv.2013.03.001
[17] Balagurunathan, R., Radhakrishnan, M., Shanmugasundaram, T., Gopikrishnan, V., & Jerrine, J. (2020). Protocols in actinobacterial research. Springer.
[18] Afolayan, M. O., Omosimua, R. O., Fadeyi, A. E., Aguzue, O. C., Asekun, O. T., & Familoni, O. B. (2022). Phytochemical, antioxidant, and flavonoid investigation of methanolic leaf extract of Piliostigma thonningii. In M. Afolayan (Ed.), Natural product experiments in drug discovery (pp. 1–26). Springer. https://doi.org/10.1007/s00044-018-2238-1
[19] Kitima, S., Souksanh, N., & Yupaporn, S. (2018). Based DPPH assay for antioxidant activity analysis. Analytical Sciences, 34(7), 795–800. https://doi.org/10.2116/analsci.18p014
[20] Molyneux, P. (2004). The use of the stable free radical DPPH for estimating antioxidant activity. Songklanakarin Journal of Science and Technology, 26(2), 211–219.
[21] Rubab, M., Chelliah, R., & Oh, D.-H. (2022). Screening for antioxidant activity: DPPH assay. In R. Balagurunathan (Ed.), Methods in actinobacteriology (pp. 453–454). Springer. https://doi.org/10.1007/978-1-0716-1728-1_61
[22] Prasanth, M., Chandini, C., & Leeja, L. (2022). Evaluation of in vitro antioxidant activity of Oldenlandia dineshii. In M. Afolayan (Ed.), Natural product experiments in drug discovery (pp. 203–214). Springer. https://doi.org/10.1007/978-1-0716-2683-2_14
[23] Oyaizu, M. (1986). Studies on products of browning reaction: Antioxidative activities of products of browning reaction prepared from glucosamine. The Japanese Journal of Nutrition and Dietetics, 44(6), 307–315. http://dx.doi.org/10.5264/eiyogakuzashi.44.307
[24] Bougandoura, N., & Bendimerad, N. (2013). Evaluation de l'activité antioxydante des extraits aqueux et méthanolique de Satureja calamintha ssp. Nepeta. Nature & Technology, 9, 14–19.
[25] Nair, S. S., Kavrekar, V., & Mishra, A. (2013). In vitro studies on alpha amylase and alpha glucosidase inhibitory activities of selected plant extracts. European Journal of Experimental Biology, 3(1), 128–132.
[26] Dastjerdi, Z. M., Namjoyan, F., & Azemi, M. E. (2015). Alpha amylase inhibition activity of Teucrium species extracts. European Journal of Biological Sciences, 7(1), 26–31.
[27] Kotowaroo, M., Mahomoodally, M., Gurib-Fakim, A., & Subratty, A. (2006). Screening of traditional antidiabetic medicinal plants of Mauritius for alpha-amylase inhibitory effects. Phytotherapy Research, 20(3), 228–231. https://doi.org/10.1002/ptr.1839
[28] Zia, T., Hasnain, S. N., & Hasan, S. (2001). Oral hypoglycaemic effect of Trigonella foenum-graecum in mice. Journal of Ethnopharmacology, 75(2-3), 191–195. https://doi.org/10.1016/s0378-8741(01)00186-6
[29] Gebreyohannis, T., Shibeshi, W., & Asres, K. (2014). Effects of solvent fractions of Caylusea abyssinica on blood glucose in rodents. Journal of Natural Remedies, 14(1), 67–75. https://doi.org/10.18311/jnr/2014/91
[30] Tesfaye, A., Makonnen, E., & Gedamu, S. (2016). Hypoglycemic and antihyperglycemic activity of Justicia schimperiana leaves extract in mice. International Journal of Pharmaceutical Sciences and Research, 7(2), 110–113. https://doi.org/10.1016/j.metop.2021.100118
[31] Tabet, A., & Boukhari, A. (2019). Estimation of total phenolic content of Tamarix gallica. World Journal of Environmental Biosciences, 8(1), 25–29.
[32] Fellah, O., Hameurlaine, S., Gherraf, N., Zellagui, A., Ali, T., Abidi, A., et al. (2018). Anti-proliferative activity of Tamarix gallica. Acta Scientiarum Naturalis, 5(2), 23–31. https://doi.org/10.2478/asn-2018-0017
[33] Tabet, A., Boukhari, A., & Nouidjem, Y. (2018). Phenolic content, HPLC analysis and antioxidant activity of Tamarix articulata. Journal of Advanced Pharmacy Education & Research, 8(4), 101–106.
[34] Anggreni, N. G., Fadhil, N., & Prasasty, V. D. (2019). Potential antioxidant activities of Piper crocatum and Persea americana leaves. Biomedical and Pharmacology Journal, 12(2), 661–667. https://doi.org/10.13005/bpj/1686
[35] Vijayalakshmi, M., & Ruckmani, K. (2016). Ferric reducing antioxidant power assay in plant extract. Bangladesh Journal of Pharmacology, 11(3), 570–572. https://doi.org/10.3329/bjp.v11i3.27663
[36] Lin, F., & Qiang, H. (2014). Interaction between tannic acid and pancreatic α-amylase. Science and Technology of Food Industry, 35(3), 63–66.
[37] Kaeswurm, J. A., Claasen, B., Fischer, M. P., & Buchweitz, M. (2019). Interaction of phenolic compounds with pancreatic α-amylase. Journal of Agricultural and Food Chemistry, 67(40), 11108–11118. https://doi.org/10.1021/acs.jafc.9b04798
[38] Sales, P. M., Souza, P. M., Simeoni, L. A., Batista, P. O. M. D., & Silveira, D. (2012). α-Amylase inhibitors: A review of plant sources. Journal of Pharmacy & Pharmaceutical Sciences, 15(3), 360–383. https://doi.org/10.18433/j35s3k
[39] Lo Piparo, E., Scheib, H., Frei, N., Williamson, G., Grigorov, M., & Chou, C. J. (2008). Flavonoids for controlling starch digestion: Structural requirements for inhibiting human α-amylase. Journal of Medicinal Chemistry, 51(12), 3555–3561. https://doi.org/10.1021/jm800115x
[40] Huang, Y., Condict, L., Richardson, S. J., Brennan, C. S., & Kasapis, S. (2023). Inhibitory mechanism of p-coumaric acid on α-amylase. Food Hydrocolloids, 142, 108524. https://hdl.handle.net/10779/rmit.27569250

Details

Primary Language

English

Subjects

Plant Biochemistry

Journal Section

Research Article

Authors

Mohammed Tahar Ben Moussa ^*
0000-0002-0316-2887
Algeria

Chihab Eddine Zeggada
0009-0002-0414-5904
Algeria

Abdallah Mouadh Rahmani
0009-0006-0709-2624
Algeria

Hassina Harkat
0000-0001-6181-1497
Algeria

Safa Chaira
0009-0008-2958-3103
Algeria

Nawal Achachi
0009-0000-2451-3968
Algeria

Publication Date

February 27, 2026

Submission Date

November 6, 2025

Acceptance Date

February 5, 2026

Published in Issue

Year 2026 Volume: 47 Number: 1

DOI

https://doi.org/10.17776/csj.1818332

IZ

https://izlik.org/JA92DG92SD

APA

Ben Moussa, M. T., Zeggada, C. E., Rahmani, A. M., Harkat, H., Chaira, S., & Achachi, N. (2026). The Hypoglycaemic Effect and Antioxidant and Anti-α-amylase Activity of the Hydroalcoholic Extract of Tamarix algeriensis G.Nicholson Tamaricaceae. Cumhuriyet Science Journal, 47(1), 55-64. https://doi.org/10.17776/csj.1818332

AMA

1.Ben Moussa MT, Zeggada CE, Rahmani AM, Harkat H, Chaira S, Achachi N. The Hypoglycaemic Effect and Antioxidant and Anti-α-amylase Activity of the Hydroalcoholic Extract of Tamarix algeriensis G.Nicholson Tamaricaceae. CSJ. 2026;47(1):55-64. doi:10.17776/csj.1818332

Chicago

Ben Moussa, Mohammed Tahar, Chihab Eddine Zeggada, Abdallah Mouadh Rahmani, Hassina Harkat, Safa Chaira, and Nawal Achachi. 2026. “The Hypoglycaemic Effect and Antioxidant and Anti-α-Amylase Activity of the Hydroalcoholic Extract of Tamarix Algeriensis G.Nicholson Tamaricaceae”. Cumhuriyet Science Journal 47 (1): 55-64. https://doi.org/10.17776/csj.1818332.

EndNote

Ben Moussa MT, Zeggada CE, Rahmani AM, Harkat H, Chaira S, Achachi N (February 1, 2026) The Hypoglycaemic Effect and Antioxidant and Anti-α-amylase Activity of the Hydroalcoholic Extract of Tamarix algeriensis G.Nicholson Tamaricaceae. Cumhuriyet Science Journal 47 1 55–64.

IEEE

[1]M. T. Ben Moussa, C. E. Zeggada, A. M. Rahmani, H. Harkat, S. Chaira, and N. Achachi, “The Hypoglycaemic Effect and Antioxidant and Anti-α-amylase Activity of the Hydroalcoholic Extract of Tamarix algeriensis G.Nicholson Tamaricaceae”, CSJ, vol. 47, no. 1, pp. 55–64, Feb. 2026, doi: 10.17776/csj.1818332.

ISNAD

Ben Moussa, Mohammed Tahar - Zeggada, Chihab Eddine - Rahmani, Abdallah Mouadh - Harkat, Hassina - Chaira, Safa - Achachi, Nawal. “The Hypoglycaemic Effect and Antioxidant and Anti-α-Amylase Activity of the Hydroalcoholic Extract of Tamarix Algeriensis G.Nicholson Tamaricaceae”. Cumhuriyet Science Journal 47/1 (February 1, 2026): 55-64. https://doi.org/10.17776/csj.1818332.

JAMA

1.Ben Moussa MT, Zeggada CE, Rahmani AM, Harkat H, Chaira S, Achachi N. The Hypoglycaemic Effect and Antioxidant and Anti-α-amylase Activity of the Hydroalcoholic Extract of Tamarix algeriensis G.Nicholson Tamaricaceae. CSJ. 2026;47:55–64.

MLA

Ben Moussa, Mohammed Tahar, et al. “The Hypoglycaemic Effect and Antioxidant and Anti-α-Amylase Activity of the Hydroalcoholic Extract of Tamarix Algeriensis G.Nicholson Tamaricaceae”. Cumhuriyet Science Journal, vol. 47, no. 1, Feb. 2026, pp. 55-64, doi:10.17776/csj.1818332.

Vancouver

1.Mohammed Tahar Ben Moussa, Chihab Eddine Zeggada, Abdallah Mouadh Rahmani, Hassina Harkat, Safa Chaira, Nawal Achachi. The Hypoglycaemic Effect and Antioxidant and Anti-α-amylase Activity of the Hydroalcoholic Extract of Tamarix algeriensis G.Nicholson Tamaricaceae. CSJ. 2026 Feb. 1;47(1):55-64. doi:10.17776/csj.1818332

As of 2026, Cumhuriyet Science Journal will be published in six issues per year, released in February, April, June, August, October, and December

The Hypoglycaemic Effect and Antioxidant and Anti-α-amylase Activity of the Hydroalcoholic Extract of Tamarix algeriensis G.Nicholson Tamaricaceae

Abstract

Keywords

Supporting Institution

Ethical Statement

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

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