Analysis of Hydroxymethylfurfural in Honey-Flavored Syrups by HPLC

Ünal Savsar; Bilal Yılmaz

doi:10.17776/csj.1840832

Analysis of Hydroxymethylfurfural in Honey-Flavored Syrups by HPLC

Abstract

The aim of this study was to develop and validate a rapid, simple and reliable high-performance liquid chromatography (HPLC) method for the accurate determination of hydroxymethylfurfural (HMF) in commercially available honey-flavored syrups. Analyses were performed using a reverse-phase HPLC system equipped with a 250 × 4.6 mm, 5 µm C₁₈ column and a UV detector set at 284 nm. The mobile phase consisted of methanol and acetonitrile (10:90, v/v), delivered at a flow rate of 1.0 mL min^-1. Under these conditions, HMF was quantified in less than 6 minutes, demonstrating the method’s efficiency and suitability for routine analysis. Method validation followed International Council for Harmonisation guidelines. The calibration curve showed linearity across 0.10-100 µg mL^-1 with a correlation coefficient above 0.99. Precision values for both intra-day and inter-day measurements were below 2.37%, while accuracy showed relative error values under 0.87%. Recovery tests, conducted using the standard addition method on three honey-flavored syrups (GrinTuss, Biakaf, Bisolnatur), confirmed the method’s robustness. Overall, the developed HPLC procedure provides a straightforward, sensitive and highly reproducible approach for determining HMF levels, supporting quality control and safety assessment of honey-flavored pharmaceutical syrups. This ensures its applicability for routine monitoring in diverse quality control settings today

Keywords

Honey-Flavored Syrup, HPLC, Hydroxymethylfurfural, Validation

Supporting Institution

The research reported in this study was funded by the Atatürk University Scientific Research Projects Coordination Unit under project number TYL-2024-14871.

Project Number

TYL-2024-14871

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Details

Primary Language

English

Subjects

Separation Science, Analytical Chemistry (Other)

Journal Section

Research Article

Authors

Ünal Savsar
0009-0008-1972-3360
Türkiye

Bilal Yılmaz ^*
0000-0002-8574-7570
Türkiye

Publication Date

April 29, 2026

Submission Date

December 15, 2025

Acceptance Date

April 12, 2026

Published in Issue

Year 2026 Volume: 47 Number: 2

DOI

https://doi.org/10.17776/csj.1840832

IZ

https://izlik.org/JA63TH24WH

APA

Savsar, Ü., & Yılmaz, B. (2026). Analysis of Hydroxymethylfurfural in Honey-Flavored Syrups by HPLC. Cumhuriyet Science Journal, 47(2), 343-349. https://doi.org/10.17776/csj.1840832

AMA

1.Savsar Ü, Yılmaz B. Analysis of Hydroxymethylfurfural in Honey-Flavored Syrups by HPLC. CSJ. 2026;47(2):343-349. doi:10.17776/csj.1840832

Chicago

Savsar, Ünal, and Bilal Yılmaz. 2026. “Analysis of Hydroxymethylfurfural in Honey-Flavored Syrups by HPLC”. Cumhuriyet Science Journal 47 (2): 343-49. https://doi.org/10.17776/csj.1840832.

EndNote

Savsar Ü, Yılmaz B (April 1, 2026) Analysis of Hydroxymethylfurfural in Honey-Flavored Syrups by HPLC. Cumhuriyet Science Journal 47 2 343–349.

IEEE

[1]Ü. Savsar and B. Yılmaz, “Analysis of Hydroxymethylfurfural in Honey-Flavored Syrups by HPLC”, CSJ, vol. 47, no. 2, pp. 343–349, Apr. 2026, doi: 10.17776/csj.1840832.

ISNAD

Savsar, Ünal - Yılmaz, Bilal. “Analysis of Hydroxymethylfurfural in Honey-Flavored Syrups by HPLC”. Cumhuriyet Science Journal 47/2 (April 1, 2026): 343-349. https://doi.org/10.17776/csj.1840832.

JAMA

1.Savsar Ü, Yılmaz B. Analysis of Hydroxymethylfurfural in Honey-Flavored Syrups by HPLC. CSJ. 2026;47:343–349.

MLA

Savsar, Ünal, and Bilal Yılmaz. “Analysis of Hydroxymethylfurfural in Honey-Flavored Syrups by HPLC”. Cumhuriyet Science Journal, vol. 47, no. 2, Apr. 2026, pp. 343-9, doi:10.17776/csj.1840832.

Vancouver

1.Ünal Savsar, Bilal Yılmaz. Analysis of Hydroxymethylfurfural in Honey-Flavored Syrups by HPLC. CSJ. 2026 Apr. 1;47(2):343-9. doi:10.17776/csj.1840832

As of 2026, Cumhuriyet Science Journal will be published in six issues per year, released in February, April, June, August, October, and December

Analysis of Hydroxymethylfurfural in Honey-Flavored Syrups by HPLC

Abstract

Keywords

Supporting Institution

Project Number

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

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