Maternal Dysbiosis Alters Systemic Osmoregulation and Organ Wet Weights Following Hemorrhagic Shock in Rats

Zeynep Semen; Gökçen Güvenç Bayram; Gözde Arslan; Fatmanur Dündar; Murat Yalçın

doi:10.17776/csj.1902177

Maternal Dysbiosis Alters Systemic Osmoregulation and Organ Wet Weights Following Hemorrhagic Shock in Rats

Abstract

Hemorrhagic shock is a major cause of trauma-related mortality, yet the contribution of the gut microbiome to systemic fluid and electrolyte regulation during acute blood loss remains unclear. This study investigated whether maternal antibiotic-induced dysbiosis alters the osmoregulatory and metabolic responses of offspring to hemorrhagic shock. Pregnant and lactating Sprague Dawley dams received vancomycin and amoxicillin, and antibiotic treatment was continued in male offspring until 8 weeks of age. Following a 4-week washout period, 12-week-old offspring underwent fixed-volume hemorrhagic shock (1.5 mL/100 g body weight), and serum biochemical variables and organ wet weights were evaluated. Compared with controls, dysbiotic offspring exhibited persistent hypernatremia and hyperchloremia (both p < 0.001), together with significantly higher calculated serum osmolarity (p = 0.030). These alterations were accompanied by significantly lower blood urea nitrogen and creatinine concentrations (p < 0.01), suggesting an attenuated metabolic and renal adaptive response. Lung and brain wet weights were also significantly lower in dysbiotic offspring (p = 0.002 and p = 0.01, respectively), whereas kidney and heart wet weights were unaffected. These findings suggest that maternal antibiotic-induced dysbiosis is associated with impaired systemic electrolyte regulation and organ-specific physiological responses during hemorrhagic stress. Clinically, closer monitoring of serum sodium and osmolarity may be warranted in trauma patients with a history of prolonged antibiotic exposure.

Keywords

Gut-lung axis, Hemorrhagic shock, Maternal dysbiosis, Osmoregulation, Pulmonary desiccation

Supporting Institution

This study was supported by the Bursa Uludağ University Scientific Research Projects (BAP) under Project Number TGA-2022-1216.

Project Number

TGA-2022-1216

Ethical Statement

The study was approved by the Bursa Uludağ University Animal Experimentation Local Ethics Committee (Approval No: 2024-03/03).

Thanks

We would like to express our gratitude to the staff of the Bursa Uludağ University Experimental Animal Application and Research Center for their expert assistance in animal care and husbandry throughout the study.

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Details

Primary Language

English

Subjects

Systems Biology, Biochemistry and Cell Biology (Other)

Journal Section

Research Article

Authors

Zeynep Semen ^*
0000-0002-7722-5772
Türkiye

Gökçen Güvenç Bayram
0000-0002-1413-3651
Türkiye

Gözde Arslan
0000-0001-7225-0138
Türkiye

Fatmanur Dündar
0000-0003-2551-3833
Türkiye

Murat Yalçın
0000-0002-5600-8162
Türkiye

Publication Date

August 31, 2026

Submission Date

March 3, 2026

Acceptance Date

August 13, 2026

Published in Issue

Year 2026 Volume: 47 Number: 4

DOI

https://doi.org/10.17776/csj.1902177

IZ

https://izlik.org/JA64HM82KG

APA

Semen, Z., Güvenç Bayram, G., Arslan, G., Dündar, F., & Yalçın, M. (2026). Maternal Dysbiosis Alters Systemic Osmoregulation and Organ Wet Weights Following Hemorrhagic Shock in Rats. Cumhuriyet Science Journal, 47(4), 683-689. https://doi.org/10.17776/csj.1902177

AMA

1.Semen Z, Güvenç Bayram G, Arslan G, Dündar F, Yalçın M. Maternal Dysbiosis Alters Systemic Osmoregulation and Organ Wet Weights Following Hemorrhagic Shock in Rats. CSJ. 2026;47(4):683-689. doi:10.17776/csj.1902177

Chicago

Semen, Zeynep, Gökçen Güvenç Bayram, Gözde Arslan, Fatmanur Dündar, and Murat Yalçın. 2026. “Maternal Dysbiosis Alters Systemic Osmoregulation and Organ Wet Weights Following Hemorrhagic Shock in Rats”. Cumhuriyet Science Journal 47 (4): 683-89. https://doi.org/10.17776/csj.1902177.

EndNote

Semen Z, Güvenç Bayram G, Arslan G, Dündar F, Yalçın M (August 1, 2026) Maternal Dysbiosis Alters Systemic Osmoregulation and Organ Wet Weights Following Hemorrhagic Shock in Rats. Cumhuriyet Science Journal 47 4 683–689.

IEEE

[1]Z. Semen, G. Güvenç Bayram, G. Arslan, F. Dündar, and M. Yalçın, “Maternal Dysbiosis Alters Systemic Osmoregulation and Organ Wet Weights Following Hemorrhagic Shock in Rats”, CSJ, vol. 47, no. 4, pp. 683–689, Aug. 2026, doi: 10.17776/csj.1902177.

ISNAD

Semen, Zeynep - Güvenç Bayram, Gökçen - Arslan, Gözde - Dündar, Fatmanur - Yalçın, Murat. “Maternal Dysbiosis Alters Systemic Osmoregulation and Organ Wet Weights Following Hemorrhagic Shock in Rats”. Cumhuriyet Science Journal 47/4 (August 1, 2026): 683-689. https://doi.org/10.17776/csj.1902177.

JAMA

1.Semen Z, Güvenç Bayram G, Arslan G, Dündar F, Yalçın M. Maternal Dysbiosis Alters Systemic Osmoregulation and Organ Wet Weights Following Hemorrhagic Shock in Rats. CSJ. 2026;47:683–689.

MLA

Semen, Zeynep, et al. “Maternal Dysbiosis Alters Systemic Osmoregulation and Organ Wet Weights Following Hemorrhagic Shock in Rats”. Cumhuriyet Science Journal, vol. 47, no. 4, Aug. 2026, pp. 683-9, doi:10.17776/csj.1902177.

Vancouver

1.Zeynep Semen, Gökçen Güvenç Bayram, Gözde Arslan, Fatmanur Dündar, Murat Yalçın. Maternal Dysbiosis Alters Systemic Osmoregulation and Organ Wet Weights Following Hemorrhagic Shock in Rats. CSJ. 2026 Aug. 1;47(4):683-9. doi:10.17776/csj.1902177

As of 2026, Cumhuriyet Science Journal will be published in six issues per year, released in February, April, June, August, October, and December

Maternal Dysbiosis Alters Systemic Osmoregulation and Organ Wet Weights Following Hemorrhagic Shock in Rats

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Ethical Statement

Thanks

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Details

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Published in Issue

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