Investigation of Antibacterial Properties of Mucus and Coelomic Fluid Obtained from Eisenia Fetida

Uğur Tutar; İsa Karaman

doi:10.17776/csj.340474

EN TR

Investigation of Antibacterial Properties of Mucus and Coelomic Fluid Obtained from Eisenia Fetida

Abstract

In several researches it was found that earthworms are protected from the infective effects of many pathogen microorganisms, with which they live together, by their strong immune systems and secreted fluids; in addition, vermicompost, their coelomic fluid and mucus are effective against certain bacteria. In this study, Disk Diffusion, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) tests were applied to determine the activities of the extracts obtained from coelom-mucus fluids of Eisenia fetida type earthworm against seven pathogenic bacteria causing diseases in plants. At the end of the study, zone diameters of the coelom-mucus fluids obtained from earthworms were measured to be between 8-54 mm, whereas their MIC and MBC values varied between 0.06-0.5 mg/mL and 0.25-2 mg/mL, respectively. The findings showed that antibacterial activity of coelom-mucus fluids obtained from E. fetida type earthworms on plant pathogens is remarkable. This study is expected to enlighten future studies investigating antimicrobial effects, structure and activity mechanisms of the isolates and extracts obtained from E. fetida and other types of earthworm.

Keywords

Eisenia fetida,coelomic fluid,mucus,antimicrobial activity

Eisenia fetida’dan Elde Edilen Sölom ve Mukus Sıvılarının Antibakteriyel Özelliklerinin Incelenmesi

Abstract

Toprak solucanlarının, birçok patojen mikroorganizma ile birlikte yaşarken bunların enfektif etkilerinden güçlü immun sistemleri ve salgıladıkları sekresyonlarla korunabildikleri, ayrıca vermikompost, sölom ve mukus sıvılarının bazı patojen bakterilere karşı etkili oldukları yapılan çeşitli araştırmalarla saptanmıştır. Bu çalışmada, E. fetida türü toprak solucanlarının sölom-mukus sıvılarından elde edilen ekstraktların, bitkilerde hastalıklara neden olan yedi adet toprak kaynaklı patojen bakteriye karşı etkinliklerinin belirlenmesi amacıyla Minimum İnhibisyon Konsantrasyon (MIC) ve Minimum Bakterisidal Konsantrasyon (MBC) testleri uygulanmıştır. Çalışma sonuçlarına göre, toprak solucanlarından elde edilen sölom ve mukus sıvılarının çalışılan bakterilere karşı zon çapları 8-54 mm arasında ölçülürken, MIC değerleri 0.06-0.5 mg/mL, MBC değerleri ise 0.25-2 mg/mL arasında bulunmuştur. Bulgular E.fetida türü toprak solucanlarından elde edilen sölom- mukus sıvılarının bitki patojenleri üzerinde antibakteriyel aktivitelerinin dikkate değer olduğunu göstermektedir. Yapılan bu çalışmanın, E. fetida’dan ve diğer toprak solucanı türlerinden elde edilecek izolat ve ekstraktların antimikrobiyal etkinliklerinin, yapılarının ve etki mekanizmalarının araştırıldığı diğer çalışmalara ışık tutması beklenmektedir.

Keywords

Eisenia fetida,sölom sıvısı,mukus,antimikrobiyal aktivite

References

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Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Uğur Tutar

İsa Karaman This is me

Publication Date

September 30, 2017

Submission Date

June 14, 2017

Acceptance Date

July 17, 2017

Published in Issue

Year 2017 Volume: 38 Number: 3

DOI

https://doi.org/10.17776/csj.340474

IZ

https://izlik.org/JA27WJ48NF

APA

Tutar, U., & Karaman, İ. (2017). Investigation of Antibacterial Properties of Mucus and Coelomic Fluid Obtained from Eisenia Fetida. Cumhuriyet Science Journal, 38(3), 427-434. https://doi.org/10.17776/csj.340474

AMA

1.Tutar U, Karaman İ. Investigation of Antibacterial Properties of Mucus and Coelomic Fluid Obtained from Eisenia Fetida. CSJ. 2017;38(3):427-434. doi:10.17776/csj.340474

Chicago

Tutar, Uğur, and İsa Karaman. 2017. “Investigation of Antibacterial Properties of Mucus and Coelomic Fluid Obtained from Eisenia Fetida”. Cumhuriyet Science Journal 38 (3): 427-34. https://doi.org/10.17776/csj.340474.

EndNote

Tutar U, Karaman İ (September 1, 2017) Investigation of Antibacterial Properties of Mucus and Coelomic Fluid Obtained from Eisenia Fetida. Cumhuriyet Science Journal 38 3 427–434.

IEEE

[1]U. Tutar and İ. Karaman, “Investigation of Antibacterial Properties of Mucus and Coelomic Fluid Obtained from Eisenia Fetida”, CSJ, vol. 38, no. 3, pp. 427–434, Sept. 2017, doi: 10.17776/csj.340474.

ISNAD

Tutar, Uğur - Karaman, İsa. “Investigation of Antibacterial Properties of Mucus and Coelomic Fluid Obtained from Eisenia Fetida”. Cumhuriyet Science Journal 38/3 (September 1, 2017): 427-434. https://doi.org/10.17776/csj.340474.

JAMA

1.Tutar U, Karaman İ. Investigation of Antibacterial Properties of Mucus and Coelomic Fluid Obtained from Eisenia Fetida. CSJ. 2017;38:427–434.

MLA

Tutar, Uğur, and İsa Karaman. “Investigation of Antibacterial Properties of Mucus and Coelomic Fluid Obtained from Eisenia Fetida”. Cumhuriyet Science Journal, vol. 38, no. 3, Sept. 2017, pp. 427-34, doi:10.17776/csj.340474.

Vancouver

1.Uğur Tutar, İsa Karaman. Investigation of Antibacterial Properties of Mucus and Coelomic Fluid Obtained from Eisenia Fetida. CSJ. 2017 Sep. 1;38(3):427-34. doi:10.17776/csj.340474