Association Between Anti-Proliferative Activity of Evernia Prunastri with the Cellular Apoptotic Pathway
Abstract
Evernia prunastri (L.) Ach, belonging to Parmeliaceae, is an
important lichen species in Turkey. Previous studies was reported that E. prunastri have significant
antioxidant, antimicrobial and anticancer compounds in its structure. Although
antiproliferative effects of E. prunastri
are determined in some types of cancer, there is little information about the
pathway of this activity. Accordingly, we aimed to determine the association
between anti-proliferative activities of E.
prunastri extracts with the cellular apoptotic pathway. Determination of
cell viability was assessed by MTT assay. The xCELLigence system was used to
monitor cell proliferation. To reveal induction apoptosis, expression of
apoptotic pathway proteins, F-actin staining, ANNEXIN
V/propodium iodide (PI) staining was used. As a result, E. prunastri MeOH extracts were found to have anti-proliferative activities
especially in high doses (p<0.05 for 1, 5 and 25 µg ml-1;
p<0.01 for 125 µg ml-1). Also, the extracts were found to be not inducing apoptosis in flow cytometric
measurements. In addition, there
was no significant decrease or increase in protein expressions of apoptotic
pathway proteins. Lastly, density of actin filaments was not affected. MeOH extract of E. prunastri was found to have
anti-proliferative activities, yet these anti-proliferative activities were not
related to cellular apoptotic pathways.
Keywords
Breast cancer,Cytotoxicity,Apoptosis,F-actine,Evernia prunastri
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