Design of Reversed Phase Chromatography (RPC) Column Packing Material for Protein Separation: Part 1- Synthesis and Characterization
Abstract
In the first part of the two-part study, the column packed
materials in the form of monodisperse and porous particles were synthesized
with regard to test their column performances in Reverse Phase Chromatography
(RPC). Particles in the form of poly(vinyl acetate-co-divinylbenzene)
[poly(VAc-co-DVB)] and poly(divinylbenzene) [poly(DVB)] were produced by
“multi-stage micro-suspension polymerization”. The surface of particles were
converted to “hydroxyl” form by base hydrolysis that were used as
poly(vinylalcohol-co-divinylbenzene) [poly(VA-co-DVB)] particles, having
appropriate structural properties like surface and porosity for chromatographic
applications. Particle characterization was assessed by Scanning Electron
Microscopy (SEM), BET surface area and porosity measurement system and Fourier
Transform Infrared Spectroscopy (FTIR).
Keywords
References
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