Design of Reversed-Phase Chromatography Column Packing Material for Protein Separation: Part 2- Chromatographic Performance
Abstract
In the second part of the two-part study, monodisperse and porous
poly(vinyl acetate-co-divinylbenzene) [poly(VAc-co-DVB)] and
poly(divinylbenzene) [poly(DVB)] column packing materials synthesized in the
first part of the study were tested in Reverse Phase Chromatography (RPC) under
different conditions. The investigation of column performances concluded that
columns packed with poly(VAc-co-DVB) (2500
plate/m) and poly(DVB) (1500
plate/m), showed high and stable theoretical plate number and high peak
resolution values compared to literature values. Protein recovery data in RPC
column, poly(VAc-co-DVB) (93-99%), were determined as similar as
quantitative values for all proteins. Reproducibility
tests of analytes of poly(VAc-co-DVB) column were assessed "run to run" and "day to day"
Relative Standard Deviation (RSD) values were determined under 2.8%.
Keywords
References
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