Determination of Glutathione Reductase Activity Changes Exposed to Some 2-Aminothiazole Derivatives
Abstract
In this work, effects of concentrations ranging
from 0 to 500 mg/L of some 2-aminothiazole derivatives such as
4,4’-(disulfanediylbis(methylene))bis(thiazol-2-amine) dihyrochloride (DMTA)
and 2-amino-4-(chloromethyl)thiazole hydrochloride (ACT) on glutathione
reductase from baker's yeast (Saccharomyces
cerevisiae) (GR) were investigated. With exposure of 25, 50, 100, 250 and
500 mg/L concentrations, % GR activity changes were calculated as -5.29 ; -3.85
; -2.40 ; -6.73 and -10.58 in DMTA applications, while these changes were
calculated as +0.98 ; 0.00 ; -0.49 ; -2.45 and 0.00 in ACT applications, respectively. This work
indicated that there was a slight decrease in GR activity with the increase of
DMTA concentrations and there was no significant change in GR activity with the
increase of ACT concentrations. But
according to control activities, no statitistical changes were observed in GR
activities with exposure of these 2-aminothiazole derivatives (p > 0.05,
n=3).
Keywords
References
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