Antimutagenic and Multi-Biological Activities of Smilax excelsa L. Fruit Extract
Abstract
In this study, antimutagenic, antimicrobial and antioxidant activities
of Smilax excelsa L. fruit extract
were evaluated. The antimicrobial effect
was investigated by disk diffusion method and the antimutagenic effect was
investigated by Ames/Salmonella/microsom test. The antioxidant properties of S.
excelsa fruit samples were determined by investigating the total phenolic,
flavonoid contents and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical
scavenging activity. It was observed
that 200 mg plate-1 fruit extract was found to have a mutagenicity
inhibition as 81% in the absence of S9 mixture and 67% in the presence of S9
mixture against Salmonella typhimurium
TA1535. S. excelsa fruit extract
produced an inhibition zone in the range of 11-16 cm against the tested
microorganisms. Flavonoid and phenolic contents were found to be 0.7985±0.0124
mgQE 100 mL-1 and 11.9847±0.0041 mgGAE 100 mL-1 at the
200 mg mL-1 extract concentration, respectively. The DPPH removal
rate was determined to be 55% at the 200 mg L-1 dose. As a result,
it was observed that the S. excelsa fruit
tissues exhibited a highly antimutagenic activity and has been determined as a
potential natural antimicrobial and antioxidant source.
Keywords
Ames/Salmonella/microsomal test,antioxidant activity,disc diffusion test
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