Investigation of the effect of Aflatoxin B1 and Aflatoxin G1 on DNA Hybridization by Using Electrochemical DNA Biosensor
Abstract
Biomolecular
detection has become a current issue together with the increase in
electrochemical sensor studies. The effect of Aflatoxin B1 and Aflatoxin G1 on
DNA hybridization was analyzed with electrochemically by using pencil graphite
electrode. For this purposes; the changes in guanine currents were monitored
before and after hybridization occurred between DNA complementary base
sequences using differential pulse voltammetry (DPV). In the first step of this
work the capture probe was immobilized by wet adsorption onto the surface of
PGE optimizing concentration and immobilization time and then hybridization
event was performed between capture and its target probe. In the second step
the hybridization experiments were repeated using inosine modified capture
probe instead of capture probe which used in the first step. As differently
from the first step the inosine modified probe was interacted with 2 µg/mL and
4 µg/mL of AFB1 for 10 min before allowing hybridization and the same protocol
was repeated for AFG1. The decrease in guanine current after hybridization was
the reference for the genotoxic effects of aflatoxins. The decrease in guanine
oxidations in the cases of 2 µg/mL and 4 µg/mL AFG1 and 2 µg/mL and 4 µg/mL
AFB1 concentrations were found to be 18% and 26 % for AFG1; and 50% and 61% for
AFB1, respectively. AFB1 showed the maximum genotoxic effect to DNA
hybridization.
Keywords
Aflatoxin B1,Aflatoxin G1,Pencil Graphite Electrode,Hybridization,Differential Pulse Voltammetry
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