Investigation of lactobacillus gasseri ma-2 as probiotic candidate
Abstract
Characterization of lactic acid bacteria in human milk has become an interesting field for developing new probiotics. Therefore, some probiotic properties of Lactobacillus gasseri MA-2 strain isolated before from human breast milk were screened in vitro. MA-2 strain exhibited safety aspects with no hemolytic activity (γ-hemolytic activity) and sensitivity to penicillin G, cloxacillin and chloramphenicol. The remarkable tolerance to various acid, bile, pepsin and pancreatin conditions was determined with high survival rate. The strain also presented significant auto-aggregation (93%) as well as co-aggregation activity with pathogenic microorganisms. L. gasseri MA-2 originated from human milk exhibited promising probiotic properties.
Keywords
probiotic,human milk,survival,gastrointestinal condition,aggregation
References
- [1] Dai D. and Walker W. A., Protective nutrients and bacterial colonization in the immature human gut, Advances in Pediatrics, 46 (1999) 353–382.
- [2] Martín R., Langa S., Reviriego C., Jiménez E., Marín M.L., Xaus J., Fernández L. and Rodríguez J.M., Human milk is a source of lactic acid bacteria for the infant gut, Journal of Pediatrics, 143 (2003) 754-758.
- [3] Jeurink P.V., van Bergenhenegouwen J., Jimenez E., Knippels L.M, Fernandez L., Garssen J., Knol J., Rodriguez J.M. and Martin R., Human milk: a source of more life than we imagine, Benef. Microbes, 4 (2013) 17–30.
- [4] Fitzstevens J.L., Smith K.C., Hagadorn J.I.,Caimano M.J., Matson A.P. and Brownell E.A., Systematic review of the human milk microbiota, Nutr. Clin. Pract., 32 (2017) 354–364.
- [5] Perez P.F., Dore J., Leclerc M., Levenez F., Benyacoub J., Serrant P., Segura-Roggero I, Schiffrin E.J. and Donnet-Hughes A., Bacterial imprinting of the neonatal immune system: lessons from maternal cells?, Pediatrics, 119 (2007) e724–e732.
- [6] Chiu Y.-H., Tsai J.-J., Lin S.-L., Choritosvakin C. and Lin M.-Y., Characterisation of bifidobacteria with immunomodulatory properties isolated from human breast milk, J. Funct. Foods, 7 (2014) 700–708.
- [7] Moles L., Escribano E., Andrés, J. De Montes M.T., Rodríguez J.M., Jiménez E., Sáenz de Pipaón M. and Espinosa-Martos I., Administration of Bifidobacterium breve PS12929 and Lactobacillus salivarius PS12934, two strains isolated from human milk, to very low and extremely low birth weight preterm infants: a pilot study, J. Immunol. Res., (2015) 538171. doi: 10.1155/2015/538171
- [8] Shin S.P., Choi Y.M., Kim WH., Hong S.P., Park J.M., Kim J., Kwon O., Lee E.H. and Hahm K.B., A double blind, placebo-controlled, randomized clinical trial that breast milk derived-Lactobacillus gasseri BNR17 mitigated diarrhea-dominant irritable bowel syndrome, J. Clin. Biochem. Nutr., 62 (2018) 179–186.
- [9] Martín R., Langa S., Reviriego C., Jiménez E., Marín M.L., Olivares M., Boza J., Jiménez J., Fernandez L. and Xaus J., The comensal microflora of human milk: new perspectives for food bacteriotherapy and probiotics, Trends Food Sci. Technol., 15 (2004) 121–127.
- [10] Morelli L. and Capurso L., FAO/WHO guidelines on probiotics 10 years latter, J. Clin. Gastroenterol., 46 (2012) 1–2.