Simultaneous determination of sulfachloropyridazine and trimethoprim in veterinary formulations by hplc
Abstract
In this study, a simple, sensitive, precise, rapid and reversed-phase high performance liquid chromatographic method with ultraviolet detection for simultaneous determination of Sulfachloropyridazine and Trimethoprim is developed and applied commercial veterinary formulation for chromatographic separation, Thermoscientific Hypersil-C18, reverse phase column was used. Separation was done using acetonitrile: pH 3.0 buffer solution (30:70, v/v) at 0.8 ml min-1 flow rate. Analysis was done 272.0 nm at ultraviyolet detector. Under optimum chromatographic conditions, retention time of Sulfachloropyridazine and Trimethoprim were determined as 4.37 and 2.60 minute. The proposed method was linear in the range 1.0-100.0 μg ml-1 for Sulfachloropyridazine and 0.5-40.0 μg ml-1 for Trimethoprim. The Limit of detection of Sulfachloropyridazine and Trimethoprim are 0.05 μg ml-1, 0.18 μg ml-1, respectively. The method which is rapid, simple and does not require any separation step, has been successfully applied to assay of commercial dosage forms containing Sulfachloropyridazine and Trimethoprim.
Keywords
Determination,HPLC,Sulfachloropyridazine,Veterinary formulation,Trimethoprim
References
- [1] Plumb D.C, Veterinary Drug Handbook (3rd ed.), Iowa State University Press, Ames, (1999) 584-587.
- [2] Kanbur M., Liman C., Eraslan G., et al. Effects of Various Cooking and Freezing Processes on the Residues of Sulfachlorpyridazine-Trimethoprim Combination in Broiler Tissues. Kafkas Univ. Vet Fak Derg , 20 (4) (2010) 487-492.
- [3] Agrawal K. V., Analysis of Antibiotic/Drug Residues in Food Products of Animal origin, (1st ed), Plenum Press, New York, (1991) 11-140.
- [4] Hess M. M., Boucher B.A., Laizure S. C., et al. Trimethoprim-sulfamethoxazole pharmacokinetics in trauma patients. Pharmacotherapy. 13(6) (1993) 602-606.
- [5] Stephen H., Kenneth H., in Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases (Eighth Edition), Elsevier Inc. (2015).
- [6] Zhang W., Duan C., Wang M., Analysis of seven sulphonamides in milk by cloud point extraction and high performance liquid chromatography. Food Chemistry, 126 (2011) 779-785.
- [7] Blackwell A.P, Holten Lützhøft C.H, Mab P.H. et al. Fast and robust simultaneous determination of three veterinary antibiotics in groundwater and surface water using a tandem solid-phase extraction with high-performance liquid chromatography–UV detection. J Chromatography A, 1045 (2004) 111–117.
- [8] Kristof E. M, Zhang G.F, Lambert W.E. Quantitative analysis of twelve sulfonamides in honey after acidic hydrolysis by high-performance liquid chromatography with post-column derivatization and fluorescence detection. J Chromatography A, 1047 (2004) 85–92.
- [9] Amini H., Ahmadiani A., Rapid and simultaneous determination of sulfamethoxazole and trimethoprim in human plasma by high-performance liquid chromatography. J Pharmaceutical and Biomedical Analysis, 43 (2007) 1146–1150.
- [10] Pereira A.V, Cass Q.B, High-performance liquid chromatography method for the simultaneous determination of sulfamethoxazole and trimethoprim in bovine milk using an on-line clean-up column. J Chromatography B, 826 (2005) 139–146.