Evaluation of tetracycline, oxytetracycline and penicillin G residues in chicken meat samples by high performance liquid chromatography with pda detection
Abstract
Penicillin G, tetracycline and oxytetracycline are broad-spectrum antibiotics with considerably low side effects which are used in most of the poultries. Due to complex matrix effects, it is considerably difficult to isolate these compounds from other molecules (such as proteins and lipids) in order to analyze. In this study, a high performance liquid chromatography (HPLC) coupled with photodiode array (PDA) detector system was introduced for the determination of tetracycline, oxytetracycline and penicillin G residues in nine chicken breast samples. For the separation of tetracycline and oxytetracycline out of samples prior to the analysis, trichloroacetic acid (TCA) and McIlvaine buffer solutions were used while formic acid and phosphate buffer solutions were utilized for penicillin G using a solid phase extraction system. The peaks at the chromatograms were enlightened and recovery percentages were calculated using spiked samples (96.57% for penicillin G; 99.00% for oxytetracycline; 95.92% for tetracycline). LOD and LOQ values were calculated, respectively as 1.55 gL-1 and 5.20 gL-1 for tetracycline; 1.32 gL-1 and 4.39 gL-1 for oxytetracycline; 1.07 gL-1 and 3.60 gL-1 for penicillin G. With the proposed method, the determination of three antibiotic residues was performed in less than 15 minutes and applied successfully to real samples.
Keywords
antibiotic residue, analysis, hplc-pda, solid phase extraction
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