Investigation of potential DNA damaging and apoptotic effects of PLK1 inhibitor SBE13 in breast cancer cell line MDA-MB-231
Abstract
Polo-like kinase 1 (PLK1) regulates various steps of mitosis and aberrantly expressed in several tumor types. As elevated levels of PLK1 contributes to tumorigenesis and poor prognosis, specific inhibition of PLK1 has garnered increasing attention in recent years in anticancer studies. The objective of this study was to examine cytotoxic, apoptotic, and DNA-damaging potentials of SBE13, a PLK1 inhibitor, against MDA-MB-231 breast cancer cells. The regulatory efficacy of SBE13 on cell cycle arrest was also determined. Cytotoxicity of SBE13 was assessed via XTT assay. Apoptosis, cell cycle distribution, and DNA damage response were also examined using the flow cytometry assay. The results revealed that SBE13 had a dose-dependent cytotoxic effect in MDA-MB-231 cells. This compound has also induced cell cycle arrest at the G2/M point and significantly promoted apoptosis and DNA damage response in MDA-MB-231 cells. Collectively, these data pointed out that SBE13 can be regarded as a suitable candidate for the therapy of breast cancer. However, further studies are required to consolidate the anticancer activity of SBE13.
Keywords
PLK1 inhibition, SBE13, Breast Cancer, Apoptosis, DNA damage
Supporting Institution
Cumhuriyet University Scientific Research Project (CUBAP)
Project Number
ECZ-045
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