Total Phenolic Content, Free Radical Scavenging Activity and Antibacterial Activity of Some Buplerum Species
Abstract
The aim of this research was to determine the total phenolic content, free radical scavenging activity and antibacterial activity of some Bupleurum species. In the study, twelve Bupleurum L. (Apiaceae) species were extracted using a mixture of methanol-water and tested for total phenolic content, free radical scavenging activity and antibacterial activity. Total phenolic content of Bupleurum species extracts ranged between 56.64 and 317.54 mg gallic acid equivalent (GAE)/g dry extract. The free radical scavenging activity of extracts was determined using the IC50 method, and the results for the samples ranged from 0.39 to 3.41 mg/ml. The antibacterial properties of Bupleurum species extracts were tested using the microdilution method against a total of eleven bacterial strains in this study; no inhibition was observed in the range of the investigated extract concentrations. Bupleurum species were contained phenolic compounds and had some free radical scavenging potential, but they did not show inhibitory impact on bacteria in the investigated extract concentration range. All the analyses were made in three replications. Results were provided as a mean of the replicates. The methods for results were looked at by utilizing the single direction and multivariate investigation of difference (MANOVA) followed by Duncan's different reach tests. The contrasts between singular methods were considered to be critical at P < 0.05.
Keywords
Antibacterial activity, Antioxidant activity, Bupleurum species, Extract, Microdilution.
References
- [1] Kubeczka K.H., Aromatic plants, basic and applied aspects. The Hague, Boston, London: Martinus Nijhoff Publishers, (1982) 165.
- [2] Nose M., Amagaya S., Ogihara Y., Corticosterone secretion-inducing activity of saikosaponin metabolites formed in the alimentary trac., Chemistry Pharmavcy Bulletin, 37 (1989) 2736-40.
- [3] Davis P.H., Turkey and the East Aegean Islands. University Press: Edinburgh, 4 (1982) 393-418.
- [4] Güner A., Özhatay N., Ekim T., Başer K.H.C., Flora of Turkey and the East Aegean Islands. University Pres: Edinburgh, 11 (2000) 143-144.
- [5] Tseng C.Y., Liu C.T., Chen W.L., Weng Y.M., Antioxidative properties and in vitro hepato-protective activity of Bupleurum kaoi leaf infusion, IFT Annual Meeting, Las Vegas, NV, July 12-16, (2004).
- [6] Lin C.C., Chiu H.F., Yen M.H., Wu C.C., Chen M.F., The pharmacological and pathological studies on Taiwan folk medicine (III): The effects of Bupleurum kaoi and cultivated Bupleurum falcatum var. komarowi, Amer. J. Chinese Med., 18 (1990) 105-112.
- [7] Ohtsu S., Izumi S., Iwanaga S., Ohno N., Yadomae T., Analysis of mitogenic substances in Bupleurum chinenese by ESR spectroscopy, Biol. Pharm. Bull., 20 (1997) 97-100.
- [8] Muller F.L., Lustgarten M.S., Jang Y., Richardson A., Van Remmen H., Trends in oxidative aging theories, Free Radic. Biol. Med., 43 (2007) 477-503.
- [9] Lin M.T., Beal M.F., The oxidative damage theory of aging, Clin. Neurosci. Res., 2 (2003) 305-315.
- [10] Yen G.C., Duh P.D., Scavenging effect of methanolic extracts of peanut hulls on free-radical and active-oxygen species, J. Agric. Food Chem., 42 (1994) 629-632.