The Effects of Oxytocin and Oxytocin Receptor Antagonist Atosiban on the Carbonic Anhydrase and Acetylcholinesterase Enzymes from Lung Tissues of Rats

Yıl 2017, Cilt: 38 Sayı: 3, 450 - 460, 30.09.2017

Ümit M. Koçyiğit

https://doi.org/10.17776/csj.340483

Cited By: 5

Öz

The purpose
of our studies is to assist in fully understanding the role of oxytocin in the
live metabolism by examining the effects of oxytocin, oxytocin receptor antagonist
atosiban, and oxytocin-atosiban chemicals injected to the rats on activities of
carbonic anhydrase (CA) and acetylcholinesterase (AChE) enzymes in the lung
tissue of the rats. For this purpose, 4 different groups each of
which contains 6 rats formed. (Control group, oxytocin administered group,
atosiban administered group, and both oxytocin and atosiban administered
group). The rats were necropsied after an hour from the injection of chemicals
into the rats intraperitoneally. The lung tissues of the rats were extracted.
Carbonic anhydrase and acetylcholinesterase enzymes activities were measured
for the tissues.  All the experimental
results were provided as mean ± SD (mean
standard deviation). Because of the data was
compatible with the normal range, it was tested via variance analysis (ANOVA)
and then, significance was determined by the Tukey test. Statistical
significance was identified to be p <0.05.

Accordingly, it was observed that there was neither a decrease of
enzyme activities in oxytocin and atosiban administered groups, nor a
significant change of enzyme activities in both oxytocin and atosiban
administered group. Also, we observed that oxytocin reduced carbonic
anhydrase enzyme activity as a systemic effect. We think that this result may
contribute to remedy of lung related diseases progressing with oxygen and
carbon dioxide transport disorders. 

Anahtar Kelimeler

Carbonic Anhydrase, Oxytocin, Atosiban, enzyme activity, rat

Sıçanların Akciğer Dokusunda Oksitosin ve Oksitosin Reseptör Antagonisti Atosiban’ın Karbonik Anhidraz ve Asetilkolinesteraz Enzim Aktiviteleri Üzerine Etkileri

Öz

Çalışmamızda,
sıçanlara enjekte edilen oksitosin ve oksitosin reseptör antagonisti olan atosibanın, sıçan akciğer dokusunda
karbonik anhidraz (CA) ve asetilkolinesteraz (AChE) enzim aktiviteleri üzerine
etkilerinin incelenerek, oksitosinin canlı metabolizmadaki rolünün tam olarak
anlaşılmasına yardımcı olmak amaçlanmıştır. Bu amaçla, her biri 6 adet sıçandan oluşan 4 farklı grup oluşturuldu.  (Kontrol grubu, oksitosin verilen grup,
atosiban verilen grup ve oksitosin ile atosibanın birlikte verildiği grup). Sıçanlara kimyasallar intraperitoneal olarak
enjekte edildikten bir saat sonra sıçanlar nekropsiye alındı. Sıçanların
akciğer dokuları çıkarıldı. Bu dokular için CA ve AChE aktiviteleri ölçüldü. Tüm deneysel sonuçlar ortalama±SH
(ortalamanın standart hatası) olarak ifade edildi. Veriler normal dağılıma
uygunluk gösterdiği için varyans analizi (ANOVA) ile test edildi ve daha sonra
anlamlılık Tukey test ile belirlendi. İstatistiksel anlamlılık p<0.05
düzeyinde tanımlandı. Buna göre, oksitosin ve atosiban verilen gruplarda enzim
aktivelerinde azalma, oksitosin ile atosibanın birlikte verildiği gruptaki
enzim aktivelerinde ise anlamlı bir değişiklik olmadığı görüldü.Ayrıca Oksitosinin sistemik bir etki olarak CA enzim aktivitesini azalttığını
gözlemledik. Bu sonucun akciğerle alakalı olan akciğer oksijen karbondioksit
taşınmasında bozuklukla seyreden hastalıklara katkısı olabileceğini
düşünmekteyiz.

Anahtar Kelimeler

Karbonik Anhidraz (CA), Oksitosin, Atosiban, enzim aktivite, rat

Kaynakça

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