Association Between Anti-Proliferative Activity of Evernia Prunastri with the Cellular Apoptotic Pathway

Yıl 2017, Cilt: 38 Sayı: 3, 516 - 524, 30.09.2017

Önder Yumrutaş Celal Güven Yusuf Özay İşıl Albeniz Müfide Aydoğan Ahbab İbrahim Bozgeyik Atilla Yıldız Haydar Bağış Leyla Türker Şener

https://doi.org/10.17776/csj.340502

Cited By: 2

Öz

Evernia prunastri (L.) Ach, belonging to Parmeliaceae, is an
important lichen species in Turkey. Previous studies was reported that E. prunastri have significant
antioxidant, antimicrobial and anticancer compounds in its structure. Although
antiproliferative effects of E. prunastri
are determined in some types of cancer, there is little information about the
pathway of this activity. Accordingly, we aimed to determine the association
between anti-proliferative activities of E.
prunastri extracts with the cellular apoptotic pathway. Determination of
cell viability was assessed by MTT assay. The xCELLigence system was used to
monitor cell proliferation. To reveal induction apoptosis, expression of
apoptotic pathway proteins, F-actin staining, ANNEXIN
V/propodium iodide (PI) staining was used. As a result, E. prunastri MeOH extracts were found to have anti-proliferative activities
especially in high doses (p<0.05 for 1, 5 and 25 µg ml^-1;
p<0.01 for 125 µg ml^-1). Also, the extracts were found to be not inducing apoptosis in flow cytometric
measurements. In addition, there
was no significant decrease or increase in protein expressions of apoptotic
pathway proteins. Lastly, density of actin filaments was not affected. MeOH extract of E. prunastri was found to have
anti-proliferative activities, yet these anti-proliferative activities were not
related to cellular apoptotic pathways.

Anahtar Kelimeler

Breast cancer, Cytotoxicity, Apoptosis, F-actine, Evernia prunastri

Evernia Prunastri’nin Antiproliferatif Aktivitesi ve Hücresel Apoptotik Yolak Arasindaki İlişki

Öz

Parmeliaceae’ye ait olan
Evernia prunastri (L.) Ach, Türkiye
yetişen önemli bir liken türüdür. önceki çalışmalarda E. Prunastri’nin yapısında bulanan kimyasallardan dolayı
antioksidan, antimikrobiyal ve antikanser aktivitelere sahip oldukları
belirtilmiştir. E. Prunastri’nin bazı
kanser hücre tiplerinde antiproliferatif
aktiviteleri bilinmekle birlikte bu aktivitenin yolağı hakkında bilgilerimiz
oldukça sınırlıdır. Bundan dolayı, çalışmamızda E. Prunastri ekstraktının antiproliferatif etkileri ve hücresel
apoptotik yolak arasında ilişkinin belirlenmesi amaçlanmıştır. Bu amaç için,
meme kanseri hücrelerini E. Prunastri ekstraktlarına
maruz bırakılmış ve hücre canlılığı MTT boyama ve xCELLigence system ile
belirlenmiştir. Annexine V/PI boyama ile Apoptozun indüksiyonu, westernblot ile
apoptotik proteinlerin ekspresyonu ve floresan boyama ile aktin flamentlerinin
hücresel yoğunluğu belirlenmiştir. E.
Prunastri MeOH ekstraktının yüksek dozlarda antiproliferatif etkiler
göstermiştir (1, 5 ve 25 µg ml^-1
için p<0.05 ; 125 µg ml^-1 için p<0.01). Ancak ekstrakt
uygulaması sonrasında apoptozun indüklenmediği ve apoptotik protein
seviyelerinin değişmediği belirlenmiştir. Ayrıca F-aktin seviyesininde
değişmediği gözlemlenmiştir. Sonuç olarak, E. Prunastri MeOH ekstraktının meme kanseri hücreleri üzerine
antiproliferatif bir etkiye sahip olduğu ancak bu etkinin apoptotik yolakla
ilişkili olmadığı düşünülmektedir.

Anahtar Kelimeler

Meme kanseri, Sitotoksisite, Apoptoz, F-aktin, Evernia prunastri

Kaynakça

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