Electron Paramagnetic Resonance Investigation of Radiation Effect on Certain Types of Drug Powders

Yıl 2018, Cilt: 39 Sayı: 2, 380 - 388, 29.06.2018

Şemsettin Osmanoğlu Kerem Sütçü

https://doi.org/10.17776/csj.390570

Öz

In this study, gamma rays were applied to the sulfadimethoxine, pyrazinamide, and chloramphenicol in the dose range of 1-15 kilogray, in order to
detect radiosensitivity of paramagnetic species, which are formed after
irradiation by electron paramagnetic resonance spectroscopy. Firstly, the intensities of
electron paramagnetic resonance signals of the samples as a function of the
microwave power were investigated at room temperature. While no signal was
obtained from the unirradiated samples, the irradiated samples exhibited
significant spectra. Additionally, the time dependency of the samples was
analyzed to study the stability of the radicals.

Anahtar Kelimeler

Electron Paramagnetic Resonance, Free radicals, Drug

Bazı İlaç Tozlarında Radyasyon Etkisinin Elektron Paramanyetik Rezonans İncelenmesi

Öz

Bu çalışmada, elektron paramanyetik rezonans
spektroskopisi ile ışınlama sonrası oluşan paramanyetik türlerin radyasyon
duyarlılığını saptamak için sülfadimetoksin, pirazinamid ve kloramfenikol
numunelerine 1-15 kilogray dozunda gama ışınları uygulandı. İlk olarak, numunelerin elektron paramanyetik rezonans sinyal
şiddetleri, mikrodalga gücünün bir fonksiyonu olarak oda sıcaklığında
incelendi. Işınlanmamış örneklerden herhangi bir sinyal elde edilmezken,
ışınlanmış numuneler belirgin spektrumlar sergiledi. Ek olarak, radikallerin
kararlılıklarını incelemek amacıyla numunelerin zamana bağımlılığı analiz
edildi.

Anahtar Kelimeler

Elektron Paramanyetik Rezonans, Serbest radikaller, İlaç

Kaynakça

• [1]. Gopal N.G.S., Radiation Sterilization of Pharmaceuticals and Polymers, Radiat. Phys. Chem., 12 (1978) 35-50.
• [2]. Diehl J.F., Food Irradiation-Past, Present and Future, Radiat. Phys. Chem., 63 (2002) 211-215.
• [3]. Bhat R. and Sridhar K.R., Influence of Ionizing Radiation and Conventional Food Processing Treatments on the Status of Free Radicals in Lotus Seeds: An ESR Study, J. Food Comp. Anal., 24 (2011) 563-567.
• [4]. Aleksieva K., Lagunov O., Dimov K. and Yordanov N.D., EPR Study on Non-and Gamma-Irradiated Herbal Pills, Radiat. Phys. Chem., 80 (2011) 767-770.
• [5]. Ambroż H.B., Kornacka E.M., Marciniec B. and Przybytniak G., Radical Decay in Irradiated Drugs: Flutamide, Ifosfamide, J. Radioanalyt. Nucl. Chem., 254 (2002) 293-298.
• [6]. Basly J.P., Bernard M. and Basly I., Electron Spin Resonance Detection of Radiosterilization of Pharmaceuticals: Application to four nitrofurans, Analyst, 123 (1998) 1753-1756.
• [7]. Basly J.P. and Bernard M., Radio Sterilization Dosimetry by ESR Spectroscopy: Ritodrine Hydrochloride and Comparison with other Sympathomimetics, Int. J. Pharmaceut., 149 (1997) 85-91.
• [8]. Delincée H., Analytical Methods to Identify Irradiated Food-a Review, Radiat. Phys. Chem., 63 (2002) 455-458.
• [9]. Karakirova Y. and Yordanov N., Mannitol as a Radiation Sensitive Material for Electron Paramagnetic Resonance Dosimetry, Bulgarian Chem. Commun., 47 (2015) 144-148.
• [10]. Ambroż H.B., Kornacka E.M., Marciniec B., Ogrodowczyk M. and Przybytniak, G.K., EPR Study of Free Radicals in Some Drugs γ-Irradiated in the Solid State, Radiat. Phys. Chem., 58 (2000) 357-366.
• [11]. Basly J.P., Duroux J.L. and Bernard M., Gamma Radiation Induced Effects on Metronidazole, Int. J. Pharmaceut., 139 (1996) 219-221.
• [12]. Çolak Ş. and Korkmaz M., Spectroscopic Features of Radiolytic Intermediates Induced in Gamma Irradiated Sulfatiazole: an ESR Study, Int. J. Pharmaceut., 285 (2004) 1-11.
• [13]. Miyazaki T., Arai J., Kaneko T., Yamamoto K., Gibella M. and Tilquin, B., Estimation of Irradiation Dose of Radiosterilized Antibiotics by Electron Spin Resonance: Ampicillin, J. Pharmaceut. Sci., 83 (1994) 1643-1644.
• [14]. Song K.M., Jeong E., Jeon W., Jo H. and Ban C., A Coordination Polymer Nanobelt (CPNB)-Based Aptasensor for Sulfadimethoxine, Biosen. Bioelectron. 33 (2012) 113-119.
• [15]. Zhang Y. and Mitchison D., The Curious Characteristics of Pyrazinamide: a Review, Int. J. Tuberc. Lung Dis., 7 (2003) 6-21.
• [16]. Bhaskar G., Kumar V.S. and Rao B.V., A Short Stereoselective Synthesis of (−)-Chloramphenicol and (+)-Thiamphenicol. Tetrahedron, Asymmetry, 15 (2004) 1279-1283.
• [17]. Johansson D., Jessen C.H., Pøhlsgaard J., Jensen K.B., Vester B., Pedersen E.B. and Nielsen P., Design, Synthesis and Ribosome Binding of Chloramphenicol Nucleotide and Intercalator Conjugates, Bioorg. Med. Chem. Lett., 15 (2005) 2079-2083.
• [18]. Basly J.P., Basly I. and Bernard M., Electron Spin Resonance İdentification of Irradiated Ascorbic Acid: Dosimetry And Influence of Powder Fineness, Anal. Chim. Acta, 372 (1998) 373-378.
• [19]. Çolak Ş. and Korkmaz M., Investigation of Structural and Dynamic Features of the Radicals Produced in Gamma Irradiated Sulfanilamide: an ESR Study, Int. J. Pharmaceut., 267 (2003) 49-58.
• [20]. Marrale M., Longo A., Panzeca S., Gallo S., Principato F., Tomarchio E. and Zeffiro A., ESR Response of Phenol Compounds for Dosimetry of Gamma Photon Beams, Nucl. Instrum. Meth. Phys. Res. Sect. B: Beam Interact. Mater. Atoms, 339 (2014) 15-19.
• [21]. Damian G., EPR Investigation of γ-Irradiated Anti-Emetic Drugs, Talanta, 60 (2003) 923-927.
• [22]. Polat M. and Korkmaz M., Effect of Gamma Radiation on Amlodis and Its Potential for Radiosterilization, J. Pharmaceut. Biomed. Anal., 40, (2006) 882-888.
• [23]. Mercanoğlu G.O., Özer A.Y., Çolak Ş., Korkmaz M., Özalp M., Ekizoğlu M. and Tilquin B., Radiosterilization of Sulfonamides: I: Determination of the Effects of Gamma Irradiation on Solid Sulphonamides, Radiat. Phys. Chem., 69 (2004) 511-520.
• [24]. Basly J.P., Radiation Induced Effects on Cephalosporins: an ESR Study, Int. J. Radiat. Biol., 75 (1999) 259-263.
• [25]. Gibella M., Crucq A.S., Tilquin B., Stocker P., Lesgards G. and Raffi J., Electron Spin Resonance Studies of Some Irradiated Pharmaceuticals, Radiat. Phys. Chem., 58 (2000) 69-76.
• [26]. Yurus S., Ozbey T. and Korkmaz, M., ESR Investigation of Gamma Irradiated Sulbactam Sodium, J. Pharmaceut. Biomed. Anal., 35 (2004) 971-978.
• [27]. Tuner H. and Korkmaz M., Radiostability of Butylated Hydroxytoluene (BHT): An ESR Study, Nucl. Instrum. Meth. Phys. Res. Sect. B: Beam Interact. Mater. Atoms, 258, (2007) 388-394.
• [28]. Basly J.P., Longy I. and Bernard M., Radiosterilization Dosimetry by Electron-Spin Resonance Spectroscopy: Cefotetan, Anal. Chim. Acta, 359 (1998) 107-113.