Antibacterial and Antioxidant Activities of Ocimum basilicum L. Against Mastitis Pathogens

Yıl 2018, Cilt: 39 Sayı: 3, 573 - 580, 30.09.2018

Gülten Ökmen Neslihan Balpınar

https://doi.org/10.17776/csj.409675

Cited By: 3

Öz

The most common bacterial agents cause
inflammation in the mammary gland (mastitis), which disease cause to less
yields in cows. These bacteria are Staphylococcus
aureus and Coagulase-negative Staphylococci
(CNS). Therefore, these bacteria were selected in this study. The purpose of
this study is to research the antibacterial and antioxidant potential of the
various extracts of Ocimum basilicum
L. The mastitis agents used in the study are 7 bacteria in total; 2 of them are
Staphylococcus aureus and the other 5
bacteria are CNS. The antibacterial activities studies were carried out using
Kirby-Bauer disc diffusion technique. The other antibacterial activity test
performed within the scope of this study was minimum inhibitory concentration
(MIC) test. Antioxidant activities were determined by
2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method. This study
showed that the largest inhibition zone was obtained from the methanol extract
of O. basilicum. Result of this study is 9 mm. The lowest MIC value was recorded as 3250 μg/mL. It was
found that O. basilicum used in the
study had antibacterial activity against mastitis pathogens, and high
antioxidant activity could be obtained from the water extracts (72%).
Consequently, O. basilicum is a good candidate in developing new antibacterial and
antioxidant agents.

Anahtar Kelimeler

Antibacterial activity, Antioxidant activity, Mastitis, Ocimum basilicum, Ocimum basilicum

Mastitis Patojenlerine Karşı Ocimum basilicum L.’ un Antibakteriyel ve Antioksidan Aktiviteleri

Öz

En
yaygın bakteriyel ajanlar meme bezlerinde iltihaplanmaya (mastitis) neden
olmakta, ki bu hastalık ineklerde düşük verime neden olur. Bu bakteriler Staphylococcus aureus ve
koagülaz-negatif Staphylococci (KNS)’dir. Bu çalışmanın amacı, Ocimum
basilicum L.’nin çeşitli özütlerinin antibakteriyel ve antioksidan
potansiyellerinin araştırılmasıdır. Çalışmada kullanılan mastitis
etkenleri toplam 7 bakteri olup, bunlardan 2 tanesi Staphylococcus aureus, diğer 5 bakteri ise KNS’dir. Antibakteriyel
aktivite çalışmaları Kirby-Bauer disk difüzyon metodu kullanılarak
gerçekleştirilmiştir. Bu çalışma kapsamında yapılan diğer bir antibakteriyel
aktivite testi, minimum inhibitör konsantrasyon (MİK) testidir. Antioksidan aktivite
çalışmaları 2,2-difenil-1-pikrilhidrazil (DPPH) radikal süpürme yöntemi ile
belirlenmiştir. Çalışma en geniş inhibisyon zonunun, O. basilicum’un metanol
özütünden sağlandığını göstermiştir. Bu çalışmanın sonucu 9 mm’dir. En düşük
MİK değeri 3250 µg/mL olarak kayıt edilmiştir. Çalışmada kullanılan O. basilicum, mastitis
patojenlerine karşı antibakteriyel aktiviteye sahiptir ve yüksek antioksidan
aktivite sulu özütlerden elde edilmiştir (%72). Sonuç olarak, yeni antibakteriyel ve antioksidan ajanların
geliştirilmesinde, O. basilicum’ un iyi bir aday olduğunu ortaya
koymaktadır.

Anahtar Kelimeler

Antibakteriyel aktivite, Antioksidan aktivite, Mastitis, Ocimum basilicum

Kaynakça

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