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Comparison of tul4, fopA, 16S rRNA and RD1 gene regions of Francisella tularensis strains isolated from Sivas, Turkey

Yıl 2021, Cilt: 42 Sayı: 1, 7 - 12, 29.03.2021
https://doi.org/10.17776/csj.842900

Öz

Tularemia is a zoonotic disease that can infect animals and people. It is known that tularemia outbreaks in Turkey is water-borne and causative agent F. tularensis subsp. holarctica. Eight F. tularensis were isolated from the water samples in tularemia outbreaks observed in Sivas during 2011-2013. In this study; tul4, fopA, RD1 and 16S rRNA gene regions of eight F. tularensis isolates were amplified by PCR method and investigated by sequencing method. Sequence analysis of the gene regions were compared with each other and the samples found in GenBank. All samples were found to be similar in terms of the partial sequence of the tul4 and fopA gene region. In terms of 16S rRNA gene region, Belkent2012 and Belkent2013 isolates and Çiçekoğlu and Döllük isolates were detected similar among themselves. Bahçeiçi and Karaören isolates were found similar at RD1 gene regions. In the sequence comparison of GenBank in terms of examined gene regions, 94-100% similarity was determined with F. tularensis subsp. holarctica LVS and F. tularensis subsp. holarctica PHIT-FT049. As a result of our study; the tul4 and fopA gene regions of eight F. tularensis subsp. holarctica isolates were found to have identical and different base sequence origins in 16S rRNA and RD1 gene regions in tularemia outbreaks in Sivas. In order to reveal the phylogeography of F. tularensis’ in Turkey, it is necessary to produce new isolates from epidemic regions and to investigate them with advanced molecular techniques.

Destekleyen Kurum

This work was supported by the Scientific Research Project Fund of Sivas Cumhuriyet University (CÜBAP) under the project number ECZ-054.

Teşekkür

We would lik to thank Dr. Bekir Çelebi (Public Health Institution of Turkey, National Tularemia Reference Laboratory, Ankara, Turkey) for the F. tularensis subsp. holarctica LVS strain (NCTC 10857).

Kaynakça

  • [1] Ellis J., Oyston P.C., Green M., Titball R.W., Tularemia, Clin. Microbiol. Rev., 15(4) (2002) 631-646.
  • [2] WHO, WHO Guidelines on Tularemia, France : World Health Organization, (2007) WHO/CDS/EPR/2007.7.
  • [3] Fey P.D., Dempsey M.M., Olson M.E., Chrustowski M.S., Engle J.L., Jay J.J., Dobson M.E., Kalasinsky K.S., Shea A.A., Iwen P.C., Wickert R.C., Francesconi S.C., Crawford R.M., Hinrichs S.H., Molecular analysis of Francisella tularensis subspecies tularensis and holarctica, Am. J. Clin. Pathol., 128(6) (2007) 926-935.
  • [4] Sjöstedt A., Tularemia: history, epidemiology, pathogen physiology and clinical manifestations, Ann. N. Y. Acad. Sci., 1105 (2007) 1-29.
  • [5] Jackson J., McGregor A., Cooley L., Ng J., Brown M., Ong C.W., Darcy C., Sintchenko V., Francisella tularensis subspecies holarctica, Tasmania, Australia 2011, Emerg. Infect. Dis., 18(9) (2012) 1484-1486.
  • [6] Hennebique A., Boisset S., Maurin M., Tularemia as a waterborne disease: a review, Emerg. Microbes Infect., 8(1) (2019) 1027-1042.
  • [7] Kilic S., Birdsell D.N., Karagöz A., Çelebi B., Bakkaloglu Z., Arikan M., Sahl J.W., Mitchell C., Rivera A., Maltinsky S., Keim P., Üstek D., Durmaz R., Wagner D.M., Water as source of Francisella tularensis infection in humans, Turkey, Emerg. Infect. Dis., 21(12) (2015) 2213–2216.
  • [8] Kılıç S., A General Overview of Francisella tularensis and the Epidemiology of Tularemia in Turkey, Flora., 15(2) (2010) 37-58.
  • [9] Engin A., Altuntaş E.E., Cankorkmaz L., Kaya A., Elaldı N., Şimşek H., Dökmetaş İ., Bakır M., The first Tularemia outbreak detected in Sivas province: Evaluation of 29 Cases., Klimik Journal., 24(1) (2011) 17-23.
  • [10] Şimşek H., Taner M., Karadenizli A., Ertek M., Vahaboğlu H., Identification of Francisella tularensis by both culture and real-time TaqMan PCR methods from environmental water specimens in outbreak areas where tularemia cases were not previously reported, Eur. J. Clin. Microbiol. Infect. Dis., 31(9) (2012) 2353-2357.
  • [11] Ataş M., Investigation of Francisella tularensis in Acanthamoeba Species Isolated from Various Water Samples, PD thesis, Cumhuriyet University, Health Sciences Institute, (2012).
  • [12] Broekhuijsen M., Larsson P., Johansson A., Byström M., Eriksson U., Larsson E., Prior R.G., Sjöstedt A., Titbal R.W., Forsman M., Genome-wide DNA microarray analysis of Francisella tularensis strains demonstrates extensive genetic conservation within the species but identifies regions that are unique to the highly virulent F. tularensis subsp. Tularensis, J. Clin. Microbiol., 41(7) (2003) 2924-2931.
  • [13] Wang Y., Hai R., Zhang Z., Xia L., Cai H., Liang Y., Shen X., Yu D., Genetic relationship between Francisella tularensis strains from China and from other countries, Biomed. Environ. Sci., 24(3) (2011) 310-314.
  • [14] Kumar S., Stecher G., Li M., Knyaz C., Tamura K., MEGA X: molecular evolutionary genetics analysis across computing platforms, Mol. Biol. Evol., 35(6) (2018) 1547-1549. [15] Gürcan S., Francisella tularensis and Tularemi in Turkey, Mikrobiyol. Bul., 41(4) (2007) 621-636.
  • [16] Kiliç S., Celebi B., Acar B., Ataş M., In vitro susceptibility of isolates of Francisella tularensis from Turkey, Scand. J. Infect. Dis., 45(5) (2013) 337–341.
  • [17] García Del Blanco N., Dobson M.E., Vela A.I., De La Puente V.A., Gutiérrez C.B., Hadfield T.L., Kuhnert P., Frey J., Domínguez L., Rodríguez Ferri E.F., Genotyping of Francisella tularensis strains by pulsed-field gel electrophoresis, amplified fragment length polymorphism fingerprinting, and 16S rRNA gene sequencing, J. Clin. Microbiol., 40(8) (2002) 2964-2972.
  • [18] Barut S, Cetin I., A tularemia outbreak in an extended family in Tokat Province, Turkey: observing the attack rate of tularemia, Int. J. Infect. Dis., 13(6) (2009) 745-748.
  • [19] Yeşilyurt M, Kılıç S, Cağaşar Ö, Celebi B, Gül S., Two Tick-borne Tularemia Cases in Yozgat Province, Mikrobiyol. Bul., 45(4) (2011) 746-754.
  • [20] Duzlu O, Yildirim A, Inci A, Gumussoy K.S., Ciloglu A, Onder Z., Molecular investigation of Francisella-like endosymbiont in ticks and Francisella tularensis in ixodid ticks and mosquitoes in Turkey, Vector Borne Zoonotic Dis., 16(1) (2016) 26-32.
  • [21] Karadenizli A., Forsman M., Şimşek H., Taner M., Öhrman C., Myrtennäs K., Lärkeryd A., Johansson A., Özdemir L., Sjödin A., Genomic analyses of Francisella tularensis strains confirm disease transmission from drinking water sources, Turkey, 2008, 2009 and 2012, Euro. Surveill., 20(21) (2015) 21136. [22] Gurcan S., Karabay O., Karadenizli A., Karagol C., Kantardjiev T., Ivanov I.N., Characteristics of the Turkish isolates of Francisella tularensis, Jpn. J. Infect. Dis., 61(3) (2008) 223-225.
  • [23] Olsufjev N.G., Meshcheryakova I.S., Subspecific taxonomy of Francisella tularensis McCoy and Chapin 1912, Int. J. Syst. Bacteriol., 33(4) (1983) 872-874.
  • [24] Çelebi B., Bakkaloglu Z., Ünaldı Ö., Karagöz A., Kılıç S., Durmaz, R., Determination of Isolated Francisella tularensis Subspecies in Turkey with Molecular Methods, Mikrobiyol. Bul., 54(1) (2020) 1-10.
Yıl 2021, Cilt: 42 Sayı: 1, 7 - 12, 29.03.2021
https://doi.org/10.17776/csj.842900

Öz

Kaynakça

  • [1] Ellis J., Oyston P.C., Green M., Titball R.W., Tularemia, Clin. Microbiol. Rev., 15(4) (2002) 631-646.
  • [2] WHO, WHO Guidelines on Tularemia, France : World Health Organization, (2007) WHO/CDS/EPR/2007.7.
  • [3] Fey P.D., Dempsey M.M., Olson M.E., Chrustowski M.S., Engle J.L., Jay J.J., Dobson M.E., Kalasinsky K.S., Shea A.A., Iwen P.C., Wickert R.C., Francesconi S.C., Crawford R.M., Hinrichs S.H., Molecular analysis of Francisella tularensis subspecies tularensis and holarctica, Am. J. Clin. Pathol., 128(6) (2007) 926-935.
  • [4] Sjöstedt A., Tularemia: history, epidemiology, pathogen physiology and clinical manifestations, Ann. N. Y. Acad. Sci., 1105 (2007) 1-29.
  • [5] Jackson J., McGregor A., Cooley L., Ng J., Brown M., Ong C.W., Darcy C., Sintchenko V., Francisella tularensis subspecies holarctica, Tasmania, Australia 2011, Emerg. Infect. Dis., 18(9) (2012) 1484-1486.
  • [6] Hennebique A., Boisset S., Maurin M., Tularemia as a waterborne disease: a review, Emerg. Microbes Infect., 8(1) (2019) 1027-1042.
  • [7] Kilic S., Birdsell D.N., Karagöz A., Çelebi B., Bakkaloglu Z., Arikan M., Sahl J.W., Mitchell C., Rivera A., Maltinsky S., Keim P., Üstek D., Durmaz R., Wagner D.M., Water as source of Francisella tularensis infection in humans, Turkey, Emerg. Infect. Dis., 21(12) (2015) 2213–2216.
  • [8] Kılıç S., A General Overview of Francisella tularensis and the Epidemiology of Tularemia in Turkey, Flora., 15(2) (2010) 37-58.
  • [9] Engin A., Altuntaş E.E., Cankorkmaz L., Kaya A., Elaldı N., Şimşek H., Dökmetaş İ., Bakır M., The first Tularemia outbreak detected in Sivas province: Evaluation of 29 Cases., Klimik Journal., 24(1) (2011) 17-23.
  • [10] Şimşek H., Taner M., Karadenizli A., Ertek M., Vahaboğlu H., Identification of Francisella tularensis by both culture and real-time TaqMan PCR methods from environmental water specimens in outbreak areas where tularemia cases were not previously reported, Eur. J. Clin. Microbiol. Infect. Dis., 31(9) (2012) 2353-2357.
  • [11] Ataş M., Investigation of Francisella tularensis in Acanthamoeba Species Isolated from Various Water Samples, PD thesis, Cumhuriyet University, Health Sciences Institute, (2012).
  • [12] Broekhuijsen M., Larsson P., Johansson A., Byström M., Eriksson U., Larsson E., Prior R.G., Sjöstedt A., Titbal R.W., Forsman M., Genome-wide DNA microarray analysis of Francisella tularensis strains demonstrates extensive genetic conservation within the species but identifies regions that are unique to the highly virulent F. tularensis subsp. Tularensis, J. Clin. Microbiol., 41(7) (2003) 2924-2931.
  • [13] Wang Y., Hai R., Zhang Z., Xia L., Cai H., Liang Y., Shen X., Yu D., Genetic relationship between Francisella tularensis strains from China and from other countries, Biomed. Environ. Sci., 24(3) (2011) 310-314.
  • [14] Kumar S., Stecher G., Li M., Knyaz C., Tamura K., MEGA X: molecular evolutionary genetics analysis across computing platforms, Mol. Biol. Evol., 35(6) (2018) 1547-1549. [15] Gürcan S., Francisella tularensis and Tularemi in Turkey, Mikrobiyol. Bul., 41(4) (2007) 621-636.
  • [16] Kiliç S., Celebi B., Acar B., Ataş M., In vitro susceptibility of isolates of Francisella tularensis from Turkey, Scand. J. Infect. Dis., 45(5) (2013) 337–341.
  • [17] García Del Blanco N., Dobson M.E., Vela A.I., De La Puente V.A., Gutiérrez C.B., Hadfield T.L., Kuhnert P., Frey J., Domínguez L., Rodríguez Ferri E.F., Genotyping of Francisella tularensis strains by pulsed-field gel electrophoresis, amplified fragment length polymorphism fingerprinting, and 16S rRNA gene sequencing, J. Clin. Microbiol., 40(8) (2002) 2964-2972.
  • [18] Barut S, Cetin I., A tularemia outbreak in an extended family in Tokat Province, Turkey: observing the attack rate of tularemia, Int. J. Infect. Dis., 13(6) (2009) 745-748.
  • [19] Yeşilyurt M, Kılıç S, Cağaşar Ö, Celebi B, Gül S., Two Tick-borne Tularemia Cases in Yozgat Province, Mikrobiyol. Bul., 45(4) (2011) 746-754.
  • [20] Duzlu O, Yildirim A, Inci A, Gumussoy K.S., Ciloglu A, Onder Z., Molecular investigation of Francisella-like endosymbiont in ticks and Francisella tularensis in ixodid ticks and mosquitoes in Turkey, Vector Borne Zoonotic Dis., 16(1) (2016) 26-32.
  • [21] Karadenizli A., Forsman M., Şimşek H., Taner M., Öhrman C., Myrtennäs K., Lärkeryd A., Johansson A., Özdemir L., Sjödin A., Genomic analyses of Francisella tularensis strains confirm disease transmission from drinking water sources, Turkey, 2008, 2009 and 2012, Euro. Surveill., 20(21) (2015) 21136. [22] Gurcan S., Karabay O., Karadenizli A., Karagol C., Kantardjiev T., Ivanov I.N., Characteristics of the Turkish isolates of Francisella tularensis, Jpn. J. Infect. Dis., 61(3) (2008) 223-225.
  • [23] Olsufjev N.G., Meshcheryakova I.S., Subspecific taxonomy of Francisella tularensis McCoy and Chapin 1912, Int. J. Syst. Bacteriol., 33(4) (1983) 872-874.
  • [24] Çelebi B., Bakkaloglu Z., Ünaldı Ö., Karagöz A., Kılıç S., Durmaz, R., Determination of Isolated Francisella tularensis Subspecies in Turkey with Molecular Methods, Mikrobiyol. Bul., 54(1) (2020) 1-10.
Toplam 22 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Yapısal Biyoloji
Bölüm Natural Sciences
Yazarlar

Sinem DEMİR 0000-0002-3536-9735

Mehmet ATAŞ 0000-0002-9425-0080

Proje Numarası ECZ-054
Yayımlanma Tarihi 29 Mart 2021
Gönderilme Tarihi 18 Aralık 2020
Kabul Tarihi 8 Mart 2021
Yayımlandığı Sayı Yıl 2021Cilt: 42 Sayı: 1

Kaynak Göster

APA DEMİR, S., & ATAŞ, M. (2021). Comparison of tul4, fopA, 16S rRNA and RD1 gene regions of Francisella tularensis strains isolated from Sivas, Turkey. Cumhuriyet Science Journal, 42(1), 7-12. https://doi.org/10.17776/csj.842900