Influences of basal media, growth regulators, explant type and photoperiod on callus competency and pigmentation of Alkanna orientalis L.

Cennet Yaman; Allah Bakhsh; Serkan Uranbey

EN

Influences of basal media, growth regulators, explant type and photoperiod on callus competency and pigmentation of Alkanna orientalis L.

Abstract

The goal of this research was in-vitro callus induction and red pigment production of Alkanna orientalis as a medicinal herb which belongs to Boraginaceae family containing valuable naphthoquinone derivates. The two different explants (leaf and leaf base) were subjected to abiotic factors such as different nutrient media (MS, B5 and M9), light and plant growth regulators (IAA and IBA). High frequency reproducible, prolific and compact calli formation was obtained from MS and B5 media supplemented with IAA, whereas high pigmentation was found in leaf base explants on M9 medium. Leaf base explant and dark conditions were found more effective for both callus formation and pigment production. The pigmentation at IBA was more than IAA, and the maximum level of pigmentation was observed on M9 medium with a combination of 1.0 mg/l IBA under dark. The factors that may be the most influential in the production of callus and red color pigment from A. orientalis have been determined. A. orientalis may be considered to be alternative plants for the A/S (alkannin/shikonin) production in vitro.

Keywords

Alkanna orientalis, Red pigment, Callus, Nutrient media, Leaf base.

Supporting Institution

Scientific Research Center of Yozgat Bozok University

Project Number

project no: 6602b-ZF/17-119

Thanks

The work was supported by grants from Scientific Research Center of Yozgat Bozok University (project no: 6602b-ZF/17-119).

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Cennet Yaman ^*
0000-0002-2364-8171
Türkiye

Allah Bakhsh
0000-0003-3561-7863
Türkiye

Serkan Uranbey
0000-0002-0312-8099
Türkiye

Publication Date

December 29, 2021

Submission Date

May 5, 2021

Acceptance Date

November 6, 2021

Published in Issue

Year 2021 Volume: 42 Number: 4

IZ

https://izlik.org/JA79GT55SS

APA

Yaman, C., Bakhsh, A., & Uranbey, S. (2021). Influences of basal media, growth regulators, explant type and photoperiod on callus competency and pigmentation of Alkanna orientalis L. Cumhuriyet Science Journal, 42(4), 766-774. https://izlik.org/JA79GT55SS

AMA

1.Yaman C, Bakhsh A, Uranbey S. Influences of basal media, growth regulators, explant type and photoperiod on callus competency and pigmentation of Alkanna orientalis L. CSJ. 2021;42(4):766-774. https://izlik.org/JA79GT55SS

Chicago

Yaman, Cennet, Allah Bakhsh, and Serkan Uranbey. 2021. “Influences of Basal Media, Growth Regulators, Explant Type and Photoperiod on Callus Competency and Pigmentation of Alkanna Orientalis L”. Cumhuriyet Science Journal 42 (4): 766-74. https://izlik.org/JA79GT55SS.

EndNote

Yaman C, Bakhsh A, Uranbey S (December 1, 2021) Influences of basal media, growth regulators, explant type and photoperiod on callus competency and pigmentation of Alkanna orientalis L. Cumhuriyet Science Journal 42 4 766–774.

IEEE

[1]C. Yaman, A. Bakhsh, and S. Uranbey, “Influences of basal media, growth regulators, explant type and photoperiod on callus competency and pigmentation of Alkanna orientalis L”., CSJ, vol. 42, no. 4, pp. 766–774, Dec. 2021, [Online]. Available: https://izlik.org/JA79GT55SS

ISNAD

Yaman, Cennet - Bakhsh, Allah - Uranbey, Serkan. “Influences of Basal Media, Growth Regulators, Explant Type and Photoperiod on Callus Competency and Pigmentation of Alkanna Orientalis L”. Cumhuriyet Science Journal 42/4 (December 1, 2021): 766-774. https://izlik.org/JA79GT55SS.

JAMA

1.Yaman C, Bakhsh A, Uranbey S. Influences of basal media, growth regulators, explant type and photoperiod on callus competency and pigmentation of Alkanna orientalis L. CSJ. 2021;42:766–774.

MLA

Yaman, Cennet, et al. “Influences of Basal Media, Growth Regulators, Explant Type and Photoperiod on Callus Competency and Pigmentation of Alkanna Orientalis L”. Cumhuriyet Science Journal, vol. 42, no. 4, Dec. 2021, pp. 766-74, https://izlik.org/JA79GT55SS.

Vancouver

1.Cennet Yaman, Allah Bakhsh, Serkan Uranbey. Influences of basal media, growth regulators, explant type and photoperiod on callus competency and pigmentation of Alkanna orientalis L. CSJ [Internet]. 2021 Dec. 1;42(4):766-74. Available from: https://izlik.org/JA79GT55SS

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Abstract

Project Number

Influences of basal media, growth regulators, explant type and photoperiod on callus competency and pigmentation of Alkanna orientalis L.

Abstract

Keywords

Supporting Institution

Project Number

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

IZ

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