Revealing the Genetic Variation and Allele Heterozygote Javanese and Arab Families in Malang East Java Indonesia

Yıl 2014, Cilt: 39 Sayı: 1, 39 - 47, 22.07.2014

Nila Kartika Sari Eriko Prawestiningtyas Fatchiyah Fatchiyah

https://doi.org/10.17826/cutf.79554

Öz

Purpose: The purpose of this study is to identify genetic variability the family of Javanese married with Arab and their allele patterns for paternity testing. Material and Methods: We used human white blood cell from three generations of three families, consists of:1 grandmother-mother, father-daughter2, grandfather-mother, father-daughter3, grandfather, grandmother - mother, father-son. DNA blood samples were isolated by salting out standard procedure, and amplified by PCR with applying 13 CODIS which consists of TPOX, D3S1358, FGA, D5S818, CSF1PO, D7S820, D8S1179, TH01, VWA, D13S317, D16S539, D18S51, D21S11 and amelogenin primer. The Fingerprint profile was visualized by 8% polyacrylamide gel and took the picture by ChemiDoc gel Imaging and measure the intensity band pattern by Quantity One software Results: Our result showed that the genetic variability and heterozygote allele increasing by using the 13 CODIS markers from the first generation to the next generation with paternity testing from each family were matched. Conclusion: We can conclude that in a Javanese-Arab family ethnic seems stimulate the increasing genetic variation and allele heterozygote.

Anahtar Kelimeler

Javanese - Arab Ethnics, DNA fingerprint, 13 CODIS

Malang Doğu Java Endonezya Da Java Halkı ve Arap Aileler Arasında Genetik Varyasyon ve Allel Heterozigotluğunun Açığa Çıkarılması

Öz

Amaç: Bu çalışmanın amacı araplar ile evli Javalı ailelerdeki genetik çeşitliliğin ve babalık testi allel paternlerini belirlemektir. Materyal ve Metod: Üç ailenin üç kuşağından alınan beyaz kan hücrelerini kullandık : büyükanne-anne1 , baba-kız2, büyükbaba-anne, baba-kız3,dede, büyükbaba - anne, büyükanne - anne , baba-oğul . DNA kan örnekleri standart prosedür tuzla çöktürme ile izole edildi ve TPOX , D3S1358 , FGA , D5S818 , CSF1PO , D7S820 , D8S1179 , TH01 , VWA , D13S317 , D16S539 , D18S51 , D21S11 ve amelogenin primerleriyle 13 CODIS"e uygun olarak ile PCR ile çoğaltıldı. Parmak izi profili %8 poliakrilamid jel ile görüntülendi ve ChemiDoc jel Görüntüleme sistemi tarafından görüntüler alındı ve Quantity One yazılımı ile bant paterni yoğunluğu ölçüldü. Bulgular: Onüç CODIS markırı kullanarak elde edilen sonuçlarımız, babalık testinde genetik değişkenlik ve allelik heterozigot allellerinin ilk nesilden sonraki nesile arttığını gösterdi. Sonuç: Java-arap ailesinin genetik varyasyon ve allelik heterozigiteyi artırmasına sebeb olduğu sonucuna varılmıştır.

Anahtar Kelimeler

Java-Arap etnik, DNA parmakizi; 13 CODIS

Kaynakça

• Butler JM. Forensic DNA Typing, 2nd Ed. UK, Elsevier Academic Press. 2005.
• Bhuyan DK, Sangwan ML, Gole VC, Sethi RK. Studies On Dna Fingerprinting In Murrah Buffaloes Using Microsatellite Markers. Indian Journal Of Biotechnology. 2010;9:367-70.
• Butler JM. Short Tandem Repeat Typing Technologies Used In Human Identity Testing. Supplement. 2007;43: 2.
• Lach C, Patsis T. DNA Fingerprinting. An Interactive Qualifying Project Report. Submitted To The Faculty Of Worcester Polytechnic Institute. Degree Of Bachelor Of Science. 2006.
• Mueller DL. Can Simple Population Genetic Models Reconcile Partial Match Frequencies Observed In Large Forensic Databases?. Journal Of Genetics. 2008;87:101-8.
• Yamamoto T, Mizutani M, Uchihi R, Tanaka M, Yoshimoto T, Misawa S, Saitou N, Katsumata Y. Allele Distributions And Genetic Relationship With 13 Codis Core Str Loci In Various Asian Populations In Or Near Japan. International Congress Series. 2003;1239:117– 20
• El-Morsi DA., El-Bakary AA, El Baz R. Alleles' Frequency Distribution Of Two Str Loci In Egyptian Population. Mansoura J. Forensic Med. Clin. Toxicol. 2009;17:1-13
• Abrahams Z, Benjeddau M. The Value Of Non-Codis Ministr Genotyping Systems In Forensic Casework In South Africa. African Journal Of Biotechnology. 2011;10:19908-912.
• Amal SH. Menelusuri Jejak Kehidupan Keturunan Arab-Jawa Di Luar Tembok Keraton Yogyakarta. Antropologi Indonesia. 2005;29:159-81.
• Fatchiyah, Arumingtyas AL, Widyarti S, Rahayu S. Basic Principles Of Molecular Biology Analysis. Erlangga, Jakarta. 2011.
• Klintschar M, Al-Hammadi N, Reichenpfader B. Significant Differences Between Yemenite And Egyptian Str Profiles And The Influence On Frequency Estimations In Arabs. Int J Legal Med. 2001;114:211–14.
• Meier RJ. The Nature Of Human Biological And Genetic Variability. Physical (Biological) Anthropology Encyclopedia Of Life Support Systems (Eolss). Bentayebi K. Genetic Profile Of Western Mediterranean Populations: Contribution Of Arab And Jewish Groups. The University Of Balearic Islands. 2012
• Steele B, Reynolds M. DNA Fingerprinting. An Interactive Qualifying Project Report. Submitted To The Faculty Of Worcester Polytechnic Institute. Degree Of Bachelor Of Science. 2004;1-83.
• Yazışma Adresi / Address for Correspondence: Dr. Fatchiyah Fatchiyah Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University Malang, East Java, INDONESIA. e-mail: [e-mail], and [e-mail] geliş tarihi/received :20.08.2012 kabul tarihi/accepted:24.09.2013