The Relationship Between Insulin PSTI Polymorphism and Prostate Cancer in Turkish Population

Yıl 2013, Cilt: 38 Sayı: 3, 434 - 439, 01.09.2013

Muhammed Hamza Muslumanoglu Tanju Basmaci Selma Demir Ulusal Huseyin Aslan Emre Tepeli Muhsin Ozdemir Mehmet Turgut

Öz

Purpose: There is positive correlation between insulin and various cancers such as colon, lung and endometrium. Protate cancer is the one of them. The aim of this study, to determine the incidance of INS+1127 PstI polymorphism, located in the 3" UTR of insulin gene, between prostate cancer cases and controls and to clarify if this polymorphism have any role in the etiology of prostate cancer. Methods: We investigated 71 prostate cancer cases and 119 normal controls unrelated to patients. INS+1127 PstI polymorphism was investigated with PCR and RFLP method. Results: At the end of study; INS+1127 PstI polymorphism genotypes out to be 67.6 % CC, 28.2 % CT, 4.2 % TT among prostatic cancer individuals and 78.2% CC, 21% CT, 0.8% TT among control group individuals. Conclusion: As a result, there was no significant difference between patients and control group, in the genotype distribution.

Anahtar Kelimeler

INS, polymorphism, prostate cancer, PstI

Türk Popülasyonunda PstI Polimorfizminin Prostat Kanseri ile İlişkisinin Araştırılması

Öz

Giriş: İnsülin ve kolon, akciğer ve endometrium kanserleri gibi bazı kanser türleri arasında pozitif bir korelasyon olduğu bilinmektedir. Prostat kanseri de bunlardan birisidir. Bu çalışmadaki amacımız insülin geni 3" UTR bölgesinde lokalize olan INS+1127 PstI polimosfizminin prostat kanseri etiyolojinde rolünü araştırmaktır. Materyal ve Metot: Çalışmaya 71 prostat kanseri tanısı almış hasta ve 119 normal kontrol bireyi dahil edilmiştir. INS+1127 PstI polimorfizm araştırması için PCR ve RFLP tekniği kullanılmıştır. Bulgular: Çalışmanın sonucunda INS geni PstI polimorfizmi genotipleri prostat kanserli hastalarda (n:71) %67,6 CC, %28,2 CT, %4,2 TT; kontrol grubunda (n: 119) %78,2 CC, %21 CT, %0,8 TT olarak belirlenmiştir. Sonuç: Hasta ve kontroller arasındaki genotip dağılımında istatistiksel olarak anlamlı bir farklılık bulunamamıştır (P=0,078).

Anahtar Kelimeler

INS, polimorfizm, prostat kanseri, PstI

Kaynakça

• Barbisan F, Mazzucchelli R, Scarpelli M, Lopez-Beltran A, Cheng L, Kirkali Z et al. Urothelial and incidental prostate carcinoma in prostates from cystoprostatectomies for bladder cancer: is there a relationship between urothelial and prostate cancer? BJU Int. 2009; 103: 1058-63.
• Bergman M, Ahnström M, Palmebäck Wegman P, Wingren S. Polymorphism in the manganese superoxide dismutase (MnSOD) gene and risk of breast cancer in young women. J Cancer Res Clin Oncol. 2005; 131: 439
• Claeys GB, Sarma AV, Dunn RL, Zuhlke KA, BeebeDimmer J, Montie JE et al. INSPstI polymorphism and prostate cancer in African-American men. Prostate. 2005; 65: 83-7.
• Ho GYF, Melman A, Liu SM, Li M, Yu H, Negassa A et al. Polymorphism of the insulin gene is associated with increased protate cancer risk. Br J Cancer. 2003; 88: 263Hsing AW, Chua S Jr, Gao YT, Gentzschein E, Chang L, Deng J et al. Prostate cancer risk and serum levels of insulin and leptin: a population-based study. J Natl Cancer Inst. 2001; 93: 783-9.
• Stattin P, Kaaks R. Re: Insulin resistance and prostate cancer risk. J Natl Cancer Inst. 2003; 95: 1086-7.
• Del Guidice ME, Fantus IG, Ezzat S, McKeown-Eyssen G, Page D, Goodwin PJ. Insulin and related factors in premenopausal breast cancer risk. Breast Cancer Res Treat. 1998; 47: 111-20.
• Gamayunova VB, Bobrov YuF, Tsyrlina EV, Evtushenko TP, Berstein LM. Comperative study of blood insulin levels in breast and endometrial cancer patients. Neoplasma. 1997; 44: 123-6.
• Goodwin PJ, Ennis M, Pritchard KI, Trudeau ME, Koo J, Madarnas Y et al. Fasting insulin and outcome in earlystage breast cancer: results of a prospective cohort study. J Clin Oncol. 2002; 20: 42-51.
• Josefson D. High insulin levels linked to deaths from breast cancer. BMJ. 2000; 320: 1496.
• Ruijter E, van dee Kaa C, Miller G, Ruiter D, Debruyne F, Schalken J. Molecular genetics and epidemiology of prostate carcinoma. Endocr Rev. 1999; 20: 22-45.
• Stead JD, Jeffreys AJ. Allele diversity and germline mutation at the insulin minisatellite. Hum Mol Genet. 2000; 9: 713-23.
• Denton RM, Tavaré JM. Does mitogen-activated-protein kinase have a role in insulin action? The cases for and against. Eur J Biochem. 1995; 227: 597-611.
• Lucassen AM, Screaton GR, Julier C, Elliott TJ, Lathrop M, Bell JI. Regulation of insulin gene expression by the IDDM associated, insulin locus haplotype. Hum Mol Genet. 1995; 4: 501-6.
• Papatsoris AG, Karamouzis MV, Papavassiliou AG. Novel insights into the implication of the IGF-1 network in prostate cancer. Trends Mol Med. 2005; 11: 52-5.
• Pasquali R, Casimirri F, De lasio R, Mesini P, Boschi S, Chierici R et al. Insulin regulates testosterone and sex hormone-binding globulin concentrations in adult normal weight and obese men. J Clin Endocrinol Metab. 1995; 80: 654-8.
• Strain G, Zumoff B, Rosner W, Pi-Sunyer X. The relationship between serum levels of insulin and sex hormone-binding globulin in men: The effects of weight loss. J Clin Endocrinol Metab. 1994; 79: 1173-6.
• Kanety H, Madjar Y, Dagan Y, Levi J, Papa MZ, Pariente C et al. Serum insulin-like growth factor binding protein-2 (IGFBP-2) is increased and IGFBP-3 is decreased in patients with prostate cancer: correlation with serum protate-specific antigen. J Clin Endocrinol Metab. 1993; 77: 229-33.
• Soos MA, Whittaker J, Lammers R, Ullrich A, Siddle K. Receptors for insulin and insulin-like growth factor-I can form hybrid dimmers. Biochem J. 1990; 270: 383-90.
• Bennetts ST, Tood JA. Human type 1 diabetes and the insulin gene: Principles of mapping polygenes. Annu Rev Genet. 1996; 30: 343-70.
• Inman BA, Harel F, Audet JF, Meyer F, Douville P, Fradet Y et al. Insulin like growth factor binding protein 2: an androgen-dependent predictor of prostate cancer survival. Eur Urol. 2005; 47: 695-702.
• Kennedy GC, German MS, Rutter WJ. The minisatellite in the diabetes susceptibility locus IDDM2 regulates insulin transcription. Nat Genet. 1995; 9: 293-8.
• Le Stunff C, Fallin D, Schork NJ, Bougnères P. The insulin gene VNTR is associated with fasting insulin levels and development of juvenile obesity. Nat Genet. 2000; 26: 444-6.
• LeRoith D, Roberts CT Jr. The insulin like growth factor system and cancer. Cancer Lett. 2003; 195: 127-37. van der Poel HG. Smart drugs in prostate cancer. Eur Urol. 2004; 45: 1-17.
• Wicksteed B, Herbert TB, Alarcon C, Lingohr MK, Moss LG, Rhodes CJ. Cooperativity between the preproinsulin mRNA untranslated regions is necessary for glucosestimulated translation. J Biol Chem. 2001; 276: 22553-8.
• Neuhausen SL, Slattery ML, Garner CP, Ding YC, Hoffman M, Brothman AR. Prostate cancer risk and IRS1, IRS2, IGF1, and IRS1 polymorphisms: Strong association of IRSI G972R variant and cancer risk. Prostate. 2005; 64: 168-74.
• Yazışma Adresi / Address for Correspondence: Dr. Muhammed Hamza M üslümanoğlu Yildiz Technical University, Faculty of Science and Arts, Department of Molecular Biology, Davutpasa Street 124, 34210 Merter/Istanbul, e-mail: [e-mail] geliş tarihi/received :15.12.2012 kabul tarihi/accepted:28.12.2012