Yıl 2019, Cilt 22 , Sayı 3, Sayfalar 268 - 275 2019-09-09

Deregulation of Cancer-Associated Genes in Odontogenic Cysts

Bilal EGE [1] , Önder YUMRUTAŞ [2] , İbrahim BOZGEYİK [3]


Objectives: The aim of the present study was to demonstrate the key role of differential expression levels of RB1, TP53, XIAP, BCL2 AIFM3, BAX, CASP3 and CASP9 genes in odontogenic cysts.

Materials and Methods: A total number of 15 patients who diagnosed with odontogenic cyst were enrolled for the present study. For the quantitative gene expression analysis, cyst and adjacent gingival healthy tissues of patients were collected during surgical assessments. Quantitative analysis of gene expression levels RB1, TP53, XIAP, BCL2 AIFM3, BAX, CASP3 and CASP9 were achieved real-time PCR method. For the optimization of gene expression levels GAPDH reference gene was used.

Results: Expression of both RB1 and TP53 genes were markedly diminished in odontogenic cysts tissues as compared to healthy tissues (p<0.05). Likewise, levels of CASP3 and CASP9 genes were found to be significantly reduced in odontogenic cysts tissues compared to healthy tissues (p<0.05). In contrast, expression levels of XIAP was significantly elevated (p<0.05). Although BCL2, AIFM3, and BAX genes were also differentially expressed in odontogenic cysts tissues, these variations were statistically insignificant (p>0.05). 

Conclusions: The findings of the present study indicates that RB1, TP53, XIAP, CASP3 and CASP9 genes might have chief roles in formation odontogenic cysts and responsible for the increased cell proliferation in these tissues.

Gene expression, odontojenic cysts, tumor suppressor gene
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Birincil Dil en
Konular Sağlık Bilimleri ve Hizmetleri
Bölüm Original Research Articles
Yazarlar

Orcid: 0000-0002-1279-0893
Yazar: Bilal EGE (Sorumlu Yazar)
Kurum: ADIYAMAN ÜNİVERSİTESİ, DİŞ HEKİMLİĞİ FAKÜLTESİ, KLİNİK BİLİMLER BÖLÜMÜ, AĞIZ, DİŞ VE ÇENE CERRAHİSİ ANABİLİM DALI
Ülke: Turkey


Orcid: 0000-0001-9657-8306
Yazar: Önder YUMRUTAŞ
Kurum: ADIYAMAN ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, TIBBİ BİYOLOJİ ANABİLİM DALI
Ülke: Turkey


Orcid: 0000-0003-1483-2580
Yazar: İbrahim BOZGEYİK
Kurum: ADIYAMAN ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, TIBBİ BİYOLOJİ ANABİLİM DALI
Ülke: Turkey


Tarihler

Başvuru Tarihi : 24 Mart 2019
Kabul Tarihi : 15 Nisan 2019
Yayımlanma Tarihi : 9 Eylül 2019

EndNote %0 Cumhuriyet Dental Journal Deregulation of Cancer-Associated Genes in Odontogenic Cysts %A Bilal Ege , Önder Yumrutaş , İbrahim Bozgeyik %T Deregulation of Cancer-Associated Genes in Odontogenic Cysts %D 2019 %J Cumhuriyet Dental Journal %P 1302-5805-2146-2852 %V 22 %N 3 %R doi: 10.7126/cumudj.543875 %U 10.7126/cumudj.543875