EVALUATION OF CYTOTOXICITY OF DIFFERENT UNIVERSAL BONDS USING THE XCELLIGENCE SYSTEM

Yıl 2020, Cilt: 23 Sayı: 4, 371 - 381, 31.12.2020

Sevim Atılan Yavuz Derya Surmelioglu

https://doi.org/10.7126/cumudj.792699

Öz

ABSTRACT
Objectives: The purpose of this study was to explore the cytotoxic effects of five different universal bonding agents on mouse fibroblast cell lines (L929).
Materials and Methods: Five different widely used universal adhesive systems were chosen that have different contents, pH levels, and polymerization methods. A real-time cell analyzer (RT-CES, xCELLigence; Roche Applied Science, Germany, and ACEA Biosciences, USA) was used for cytotoxic evaluation of light-cured polymerized G-Premio Bond (GC Europe, Belgium), Prime&Bond Universal (Dentsply Sirona, USA), Universal Bond Quick (Kuraray, USA), Single Bond Universal (3M ESPE, USA) and self-cured polymerized Tokuyama Universal Bond (Tokuyama, USA) experimental groups. L929 were cultured in Dulbecco’s modified Eagle’s medium and supplemented with 10% fetal bovine serum and 1 % antibiotics. The assay was performed E-plate-16 and monitored every 15 min for 72 h. Statistical analysis was performed using ANOVA and Tukey’s posthoc tests.
Results: All tested universal adhesive systems showed a statistically significant difference in cytotoxicity values in different time periods (p<0.05). Among the groups compared, G-Premio Bond showed the least cytotoxic effect; and Tokuyama Universal Bond showed the most cytotoxic effect. Different times of all universal adhesive systems significantly increased the count of viable cells compared to the control group (p<0.05).
Conclusions: In dentistry, universal adhesive systems can be observed cytotoxic effects to live cells. The evaluation of cytotoxicity with xCELLigence device is a reliable method and should be supported by new studies on this subject.

Anahtar Kelimeler

xCELLigence, cytotoxicity, universal adhesive systems, L929

Kaynakça

• 1. Schmalz G. Concepts in biocompatibility testing of dental restorative materials. Clinical Oral Investig. 1998;1:154-162
• 2. Perdigão J. Dentin bonding—Variables related to the clinical situation and the substrate treatment. Dent Mater .2010; 26:24-37
• 3. Tay FR, Pashley DH. Aggressiveness of contemporary self-etching systems: I: Depth of penetration beyond dentin smear layers. Dent Mater. 2001; 17:296-308
• 4. Hanabusa M, Mine A, Kuboki T, Momoi Y, Van Ende A, Van Meerbeck B, De Munck. Bonding effectiveness of a new ‘multi-mode’adhesive to enamel and dentine. J Dent. 2012;40:475-484
• 5. Perdigão J, Sezinando A, Monteiro PC. Laboratory bonding ability of a multi-purpose dentin adhesive. Am J Dent. 2012;25:153
• 6. Muñoz MA, Luque I, Hass V, Reis A, Loguercio AD, Bombarda NHC. Immediate bonding properties of universal adhesives to dentine. J Dent. 2013;41:404-411.
• 7. Lawson NC, Robles A, Fu CC, Lin CP, Sawlani K, Burgess JO. Two-year clinical trial of a universal adhesive in total-etch and self-etch mode in non-carious cervical lesions. J Dent. 2015;43:1229-1234.
• 8. Al‐Dawood A, Wennberg A. Biocompatibility of dentin bonding agents. Denta Traumatol. 1993;9:1-7.
• 9. Kusdemir M, Gunal S, Ozer F, Imazato S, Izutani N, Ebisu S, Blatz MB. Evaluation of cytotoxic effects of six self-etching adhesives with direct and indirect contact tests. Dent Mater J. 2011;799.
• 10. Schmalz G, Arenholt-Bindslev D. Biocompatibility of dental materials. 2009, Springer 1.
• 11. Lanza CRM, Costa CAS, Furlan M, Alecio A, Hebling J. Transdentinal diffusion and cytotoxicity of self-etching adhesive systems. Cell Biol Toxicol 2009;25:533.
• 12. Schedle A, Franz A, Raush-Fan X, Spittler A, Lucas T, Samorapoompichit P, Sperr W, Boltz-Nitulescu G. Cytotoxic effects of dental composites, adhesive substances, compomers and cements. Dental Mater. 1998;14:429-440.
• 13. Geurtsen W. Biocompatibility of resin-modified filling materials. Crit Rev Oral Biol Med. 200;11:333-355.
• 14. Nakabayashi N, Kojima K, Masuhara E. The promotion of adhesion by the infiltration of monomers into tooth substrates. J Biomed Mater Res. 1982; 16:265-273. 15. Schmalz G. Materials science: biological aspects. J Dent Res. 2002;81:660
• 16. Ratanasathien S, Wataha JC, Hanks CT, Dennison JB. Cytotoxic interactive effects of dentin bonding components on mouse fibroblasts. J Dent Res. 1995;74:1602-1606.
• 17. Tay FR, Carvalho R, Sado H, Pashley DH.Effect of smear layers on the bonding of a self-etching primer to dentin. J Adhes Dent. 200;2:99-116.
• 18. Bergenholtz G. Evidence for bacterial causation of adverse pulpal responses in resin-based dental restorations. Crit Rev Oral Biol Med. 200;11:467-480. 19. Chen, RS, Liu CC, Tseng WY, Jeng JH, Lin CP. Cytotoxicity of three dentin bonding agents on human dental pulp cells. J Dent. 2003;31:223-229.
• 20. Hashieh IA, Cosset A, Franquin JC, Camps J. In vitro cytotoxicity of one-step dentin bonding systems. J Endod. 1999;25:89-92.
• 21. Karapınar‐Kazandag M, Bayrak ÖF, Yalvaç ME, Ersev H, Tanalp J, Şahin F, Bayırlı G. Cytotoxicity of 5 endodontic sealers on L929 cell line and human dental pulp cells. Int Endod J. 2011; 44:626-634.
• 22. Yasuda Y, Inuyama H, Maeda H, Akamine A, Nör JE, Saito T. Cytotoxicity of one‐step dentin‐bonding agents toward dental pulp and odontoblast‐like cells. J Oral Rehabil. 2008;35:940-946.
• 23. Murray PE, About I, Lumley PJ, Franquin JC, remusat M, Smith AJ. Cavity remaining dentin thickness and pulpal activity. Am J Dent. 2012;15:41-46.
• 24. Albuquerque M, Pegoraro M, Mattei G, Reis A, Loguercio AD. Effect of double-application or the application of a hydrophobic layer for improved efficacy y of one-step self-etch systems in enamel and dentin. Oper Dent. 2008;33:564-570.
• 25. Perdigão J, Munoz MA, sezinando A, Luquel-Martinez IV, Staichak R, reis A, Loguerco AD. Immediate adhesive properties to dentin and enamel of a universal adhesive associated with a hydrophobic resin coat. Oper Dent. 2014;39:489-499.
• 26. Altintas SH, Usumez A. Evaluation of monomer leaching from a dual cured resin cement. J Biomed Mater Res B. 2008;86:523-529.
• 27. Chen C, Niu LN, Xie H, Zhang ZY, Zhou LQ, Jiao K, Chen JH, Pashley DH, Tay FR. Bonding of universal adhesives to dentine–Old wine in new bottles? J Dent. 2015; 43:525-536.
• 28. Ergün G, Eğilmez F, Üçtaşlı MB, Yilmaz Ş. Effect of light curing type on cytotoxicity of dentine‐bonding agents. Int Endod J. 2006;40:216-223.
• 29. Kılıç K, Kesim B, Sümer Z, Polat Z, Kesim S. Evalution of Biocompatibility of Full Ceramics with MTT. J Health Sci. 2010;19:125-132.
• 30. da Silva JM, Rodrigues JR, Camargo CHS, Fernandes VVB, Hiller KA, Schweiki H, Schmalz G. Effectiveness and biological compatibility of different generations of dentin adhesives. Clin Oral Investig. 2014;18:607-613.
• 31. Wataha J, Rueggeberg FA, Lapp CA, Lewis CA, Lockwood PE, Ergle JW, Mettenburg DJ. In vitro cytotoxicity of resin-containing restorative materials after aging in artificial saliva. Clin Oral Investig. 1999;3:144-149.
• 32. Thonemann B, Schmalz G, Hiller KA, Schweikl H. Responses of L929 mouse fibroblasts, primary and immortalized bovine dental papilla-derived cell lines to dental resin components. Dent Mater. 2002;18:318-323.
• 33. Bouillaguet S, Shaw L, Gonzalez L, Wataha JC, Krejci L. Long‐term cytotoxicity of resin‐based dental restorative materials. J Oral Rehabil. 2002;29:7-13.
• 34. Wataha JC, Craig RG, Hanks C. Precision of and new methods for testing in vitro alloy cytotoxicity. Dent Mater. 1992;8:65-70.
• 35. Ke N, Wang X, Xu X, Abassi YA. The xCELLigence system for real-time and label-free monitoring of cell viability, in Mammalian Cell Viability. Mammalian Cell Viability. 2011;740:33-43.
• 36. Urcan E, Haertel U, Styllou M, Hickel R, Scherthan H, Reichl FX. Real-time xCELLigence impedance analysis of the cytotoxicity of dental composite components on human gingival fibroblasts. Dent Mater.201;26:51-58.
• 37. Malkoç S, Öztürk F, Çörekçi B, bozkurt BS, Hakki SS. Real-time cell analysis of the cytotoxicity of orthodontic mini-implants on human gingival fibroblasts and mouse osteoblasts. Am J Orthod Dentofac. 2012;141:419-426.
• 38. Golke A, Cymerys j, Stonska A, Dzieciakowski T, Chmielewska a, Tucholska A,Banbura MW. The xCELLigence system for real-time and label-free analysis of neuronal and dermal cell response to Equine Herpesvirus type 1 infection. Pol J Vet Sci. 2012;15:151-153.
• 39. Ruyter I. Physical and chemical aspects related to substances released from polymer materials in an aqueous environment. Adv Dent Res. 1995;9:344-347.
• 40. Geurtsen W, Spahl W, Leyhausen G. Variability of cytotoxicity and leaching of substances from four light‐curing pit and fissure sealants. J Biomed Mater Res. 1999; 44:73-77.
• 41. Gerzina T, Hume W. Diffusion of monomers from bonding resin-resin composite combinations through dentine in vitro. J Dent. 1996;24:125-128.
• 42. Geurtsen W, Lehmann F, Spahl W, Leyhausen G. Cytotoxicity of 35 dental resin composite monomers/additives in permanent 3T3 and three human primary fibroblast cultures. J Biomed Mater Res. 1998; 41:474-480.
• 43. Knezevic A, Zeljezic D, Kopjar N, Tarle Z. Cytotoxicity of composite materials polymerized with LED curing units. Oper Dent. 2008;33:23-30.
• 44. Taira M, Nakao H, Matsumoto T, Takahashi J. Cytotoxic effect of methyl methacrylate on 4 cultured fibroblasts. Int J Prosthodont. 2000;13:311-315.
• 45. Koulaouzidou EA, Papazisis KT, Yiannaki E, Palaghias G, Antoniades MH. Effects of dentin bonding agents on the cell cycle of fibroblasts. J Endod.2009;35:275-279.
• 46. Rakich DR, Wataha JC, Lefebvre CA, Weller RN. Effects of dentin bonding agents on macrophage mitochondrial activity. J Endod 24(8):528-533.
• 47. Al‐Hiyasat AS, Darmani H, Elbetieha AM. Leached components from dental composites and their effects on fertility of female mice. Eur J Oral Sci. 2009;112:267-272.
• 48. Franz A, König F, Lucas T, Watts DC, Schedle A. Cytotoxic effects of dental bonding substances as a function of degree of conversion. Dent Mater. 2009;25:232-239.
• 49. Schmalz G, Schuster U, Koch A, Schwiki H. Cytotoxicity of low pH dentin-bonding agents in a dentin barrier test in vitro. J Endod. 2002; 28:188-192.