Yıl 2016, Cilt 37, Sayı 1, 30 - 40, 06.04.2016

Caner KASIMOĞLU Handan UYSAL

https://doi.org/10.17776/csj.10890

Öz

Farklı Test Sistemleri ile Somatik Hücrelerde Profenofos Genotoksisitesine Karşı Kuşburnu (Rosa canina L.) Ekstrelerinin Doğal Bir Antigenotoksik Ajan Olarak Kullanılması

Öz

Bu çalışmada, profenofos insektisitinin genotoksik etkileri, Drosophila melanogaster’de somatik mutasyon ve rekombinasyon testi (SMART) ve insan periferal lenfosit hücrelerinde mikronükleus (MN) testi ile araştırılmıştır. Bu maddenin olası genotoksik etkilerinin giderilebilmesi için de kuşburnu (Rosa canina) bitkisine ait su ve etanol ekstreleri kullanılmıştır. SMART için D.melanogaster’in kanat preparatları incelendiği zaman, profenofosun artan konsantrasyonuna (0,025, 0,05, 0,075 ve 0,1ppm) bağlı olarak normal kanat fenotipinde mutasyon frekansının arttığı özellikle en yüksek uygulama grubunda (0,1 ppm) bu artışın pozitif etkili (+) olduğu gözlenmiştir (P < 0,05). Serrat kanat fenotipinde ise mutasyon frekansındaki artış tüm uygulama gruplarında önemsiz etkili (i) bulunmuştur (P > 0,05). Kuşburnu bitkisinin su (RCsu) ve etanol (RCeta) ekstreleri, profenofosun en yüksek konsantrasyonu (0,1 ppm) ile birlikte uygulandığı zaman (profenofos+ RCsu/RCeta) mutasyon frekansındaki artışın azaldığı ve bunun da istatistiksel olarak önemli olduğu gözlenmiştir (P < 0,05). Bu çalışmada, farklı konsantrasyonlarda in vitro profenofos uygulaması (0,25, 0,5, 0,75 ve 1ppm) ile insan periferal lenfosit hücrelerinde mikronükleus frekansının arttığı ve nükleer bölünme indeksinin (NBİ) azaldığı da görülmüştür (P < 0,05). Ancak profenofos+RCsu ve RCeta uygulamaları ile mikronükleus frekansı düşerken NBİ değeri de kontrol grubuna yaklaşmıştır (P < 0,05).

Anahtar Kelimeler

Drosophila melanogaster, Profenofos, SMART, Mikronükleus, Rosa canina

Kaynakça

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