The Study of Comparative Nsc, S And Bmsc, S Neurons Therapy For Treatment of Artificially Created Neuron Disorders In Sheep

Year 2015, Volume: 36 Issue: 3, 2009 - 2015, 13.05.2015

M.e Akbarı , Sh Salehın

Abstract

The main problem with embryonic stem cell research is the tissue incompatibility. Millions of lines must be established in order to serve a significant percentage of potential patients. The use of autologous adult stem cells (cells from the patient) eliminates the problems with tumorogenesis, mutation, and tissue incompatibility.Multipotent stem cells can be found in the numerous adult tissues. The isolation and expansion of neural cell types has become increasinglyrelevant in restorative neurobiology. To examine the developmentalpotential of adult  sheep brain cells, we applied conditionsfavoring the growth of neural stem cells and bone marrow stem cells  to multiple corticalregions, resulting in the identification and selection of apopulation of adult sheep neural progenitors.. It has also been seen that BMSC can differentiate into neural cells and therefore can be used for restoration of brain damage. We tested the theory that BMSC could change into neural precursor cells by co culturing BMSC, identified by using β-galatosidase, with fetal primary neuronal cultures. We concluded that, our findings suggestan unprecedented developmental plasticity and proliferative  potential are retained in CNS glia throughout life and the use of autologous adult stem cells  eliminates the problems with tumor genesis, mutation, and tissue incompatibility in both NSC,s and ESC,s therapy.

Keywords

NSC, s, BMSC, s;Teratoma;ANHPs; β-galatosidase, sheep

References

• Jiang Y, Henderson D, Blackstad M et al. Neuroectodermal differentiation from mouse multipotent adult progenitor cells. Proc Natl Acad Sci U S A 2003;100 (Suppl 1):11854– 11860.
• Reyes M, Lund T, Lenvik T et al. Purification and ex vivo expansion of postnatal human marrow mesodermal progenitor cells. Blood 2001;98:2615–2625.
• Schwartz RE, Reyes M, Koodie L et al. Multipotent adult progenitor cells from bone marrow differentiate into functional hepatocyte-like cells. J Clin Invest 2002;109:1291– 1302.
• Scheffler B,Walton N.M.,2005.Phenotypic and functional characterization of adult brain neuropoiesis. Proc. Natl. Acad. Sci. USA 102,9353 -9358.