The effects of GA3 treatments and nutrient media on in vitro seed germination of Allium tuncelianum (Kollman), Özhatay, Matthew, Şiraneci

Abstract

In this study, the effects of GA3 and nutrient media on in vitro seed germination of Allium tuncelianum (Kollman), N. Özhatay, D. Matthew, Şiraneci, which is one of the endemic plants in Turkey, were investigated. In order to promote seed germination, GA3 was applied to seeds under sterile conditions for 48 hours at different concentrations (2 mgL-1, 3 mgL-1 and 4 mgL-1). Then the seeds were cultured in BDS and MS medium in a sterile cabinet. The results showed that the germination rate decreased with the increase of the applied concentrations of GA3. The highest germination rate (17.73%) was obtained from seeds treated with 2 mgL-1 GA3. It was determined that the effects of nutrient media on the germination of Allium tuncelianum seeds were important, and the highest germination values were obtained from the BDS medium.

Keywords

• Allium tuncelianum
• BDS
• GA3
• Germination
• MS

References

1. Aasim, M., (2015). Adventitous bulblet regeneration of endemic Ovacik garlic (Allium tuncelianum Kollman, Ozhatay, Mathew, Siraneci) using wintered half clove explant. Romanian Biotechnological Letters, 20(5), 10845‒108551.
2. Agbas, B., Karakus, D., Adiguzel, G., Keser, S., Demir, E., (2013). Comparison of total antioxidant properties and dry matter content of Tunceli garlic (Allium tuncelianum) and normal garlic (Allium sativum), Bilim ve Genclik Dergisi, 1(2), 50-62. ISSN: 2148- 0273.
3. Alper, A., (2005). Tunceli sarımsağının (Allium tuncelianum Kollman, Özhatay, Marhew, Şiraneci) bitkisel özelliklerinin belirlenmesi. Ankara Üniversitesi Fen Bilimleri Enstitüsü, Bahçe Bitkileri Anabilim Dalı, Ankara, p16.
4. Anonymous, (1985). International Rules for Seed Testing. Seed Science and Technology, 13; 421-463.
5. Arguello, J.A., Falcon, L.R., Seisdedos, L., Milrad, S., Bottini, R., (2001). Morphological changes in garlic (Allium sativum L.) microbulblets during dormancy and sprouting as related to peroxidase activity and gibberellin GA3 content. Biocell., 25: 1-9.
6. Atila, G., Uslu, H., Erdag, D., Ozkan, O., (2017). Antioxidative and antihyperglycemic effects of Allium tuncelianum on streptozotocin induced type I diabetes, International Congress on Medicinal and Aromatic Plants,p: 411, May 10-12, 2017, Konya.
7. Dunstan, D.I., Short, K.C., (1977). Improved Growth of Tissue Cultures of The Onion, Allium cepa. Physiol Plant, 41: 70-72.
8. Etoh. T., Simon, P.W., (2002) Diversity, fertility and seed production of garlic, H.D. RABINOWITCH, L. CURRAH, eds., Allium Crop Science: Recent Advances, CABI Publishing, New York, 2002, pp. 101–117.

9. Kizil, S., Icgil, D.Y., Khawar, K.M., (2014). Improved in vitro regeneration and propagation of Tunceli garlic (Allium tuncelianum L.) from sectioned garlic cloves, leaves and root explants. Journal of Horticultural Science and Biotechnology, 89(4): 408-414.
10. Kizil, S., Sogut, T., Khawar, K.M., (2017). Germination and bulb formation of Allium tuncelianum L. under in vitro condition, ICAB 2017: 19th International Conference on Agriculture and Biotechnology. 13-14 July, Stockholm, Sweden.
11. Murashige, T., Skoog, F., (1962). A Revised Medium for Rapid Growth and Bioassay With Tobacco Tissue Cultures. Physiologia Plantarum, 15: 473-497.
12. Ozhatay, N., Mathew, B., (1995). New taxa and notes on the genus Allium (Alliaceae) in Turkey and Arabia, Kew Bulletin, 50(4): 723‒731.
13. Sehitoglu, M.H., Yarali Karakan, F., Kizilkaya, B., Oztopuz, R.O., Gulcin, I., (2018). Investigation of antioxidant properties and bioactive composition of Allium tuncelianum ((kollman) ozhatay, matthew & siraneci) and allium sativum L. Journal of the Institute of Science and Technology, 8(4): 213-221.
14. Yanmaz, R., Ermiş, S., (2005). Tunceli sarımsağı (Allium tuncelianum Kollman, Özhatay,. Matthew, Şiraneci)) tohumlarındaki çimlenme probleminin çözülmesi üzerine araştırma/ Researches On Overcomıng The Germınatıon Problems Of Tuncelı Garlıc Seeds (Allium tuncelianum . (Kollman), N. Ozhatay, D. Matthew, Siranec . Türkiye 2. Tohumculuk kongresi, Adana, s: 101-106.
15. Yanmaz, R., Yazar, E., Kantoğlu, Y., and Alper, A., (2010). In vitro plant regeneration and bulblet formation of Tunceli garlic (Allium tuncelianum (Kollman) Özhatay, Matthew, Siraneci) By Shoot and Root Culture. Journal of Food, Agriculture & Environment, Vol: 8(3-4): 572- 576.
16. Yarali, F., Yanmaz, R., (2016).The effects of media composition on in vitro gynogenic embryo induction in Allium tuncelianum (Kollman) Ozhatay, Matthew, Siraneci. International Journal of Agriculture Sciences, ISSN: 0975-3710 & E-ISSN: 0975-9107, 8(58): 3247-3251.
17. Yaralı Karakan, F., (2019). A Review Of In Vitro Studies On Allium tuncelianum (Kollman) Ozhatay, Matthew, Siraneci, Scientific Bulletin. Series F. Biotechnologies, Vol. XXIII, ISSN 2285-1364, CD-ROM ISSN 2285-5521, ISSN Online 2285-1372, ISSN-L 2285-1364, p: 11-16.