The Determination of the Effects of Wild Thyme (Thymbra spicata L.) and Cumin (Cuminum cyminum L.) Extracts on SCE (Sister Chromatid Exchange) in Human Lymphocyte Chromosomes

Abstract

The purpose of this work is to determine of T. spicata L. and C. cyminum L. plant extracts on sister chromatid exchange rate in human peripheral lymphocyte culture as in vitro. Human blood lymphocyte cells, T. spicata L. and C. cyminum L. plant extracts were allowed to interact with doses of 0.05 µL/mL, 0.10 µL/mL, 0.15 µL/mL and 0.20 µL/mL for 24 hours. When trial groups where plant extracts were applied were compared with negative control and mitomycin-C (MMC) which was used as positive control, it was determined that the extract doses applied led to an increase in sister chromatid exchange rate. Also, it was found that increasing concentrations of plant extracts caused cell replication index to decrease. Between C. cyminum L. doses and RI, a negative correlation (r = -0.95) was observed and between T. spicata L. doses and RI a negative correlation was observed (r = -0.94) respectively. As a result of this study; T. spicata L. and C. cyminum L. plant extracts were found to have genotoxic and clastogenic effects on human peripheral lymphocytes.

Keywords

• Mitomycin-C,Replication Index,Genotoxic,Clastogenic

References

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