Effect of a tropical plant (Hunteria umbellata) in the management of Streptozotocin induced Diabetes Mellitus and other physiological and biochemical functions in Wistar

Year 2021, Volume: 4 Issue: 1, 1 - 12, 02.05.2021

Fidelis Okolafor , Frederick Ekhaıse

https://doi.org/10.38093/cupmap.842583

Abstract

The antidiabetic effect of Hunteria umbellata extracts (aqueous and methanol) on hematological, lipid profile and biochemical parameters in streptozotocin-induced diabetes in female wistar rats was studied. The phytochemical screening of the seed extracts was determined using standard chemical methods. Twenty five (25) wistar rats weighing between 117g and 170g were shared into five (5) groups of five animals per group for the physiological studies. All treatment groups were administered 500 mg/kg per body weight of H. umbellata extracts. Group 1: normal control, group 2: negative or diabetic control, group 3: metformin + streptozotocin (STZ), group 4: aqueous extract of H. umbellata + STZ and group 5: methanol extract of H. umbellata + STZ. Diabetes was induced with 55mg/kg body weight streptozotocin (STZ). Quantitative phytochemical screening of aqueous and methanol seed extracts of H. umbellata reviewed the presence of secondary metabolites such as, saponins, phytate, oxalate, anthraquinones, Cyanogenic glycoside; where phenols and alkaloids recorded highest concentrations in both extracts. The hypoglycaemic studies of methanol extract revealed significant (p<0.005) reduction on the fasting blood glucose levels of experimental animals for group 5 compared to moderate reduction in group 3. Group 4 recorded increase in the blood glucose levels of wistar rats. The haematological parameters were not significant (p>0.05) for all treatment groups. Group 5 recorded significance (p<0.05) amount of high density lipoproteins (HDL), total cholesterol (CHOL), triglyceride (TRIG) and low density lipoproteins (LDL) while CHOL was significant (p<0.05) for group 3. The biochemical parameters were within safe limit. The methanol extract of H. umbellata possesses the potentials for the managements of blood glucose levels compared to conventional drug (metformin).

Keywords

Antidiabetic, Phytochemical screening, Streptozotocin, wistar rats, Hunteria umbellata, Meforming

References

• 1. Adeneye, A.A. and Crooks, P.A., 2015., Weight losing, antihyperlipidemic and cardioprotective effects of the alkaloid fraction of Hunteria umbellata seed extract on normal and triton- induced hyperlipidemic rats. Asian Pacific Journal of Tropical Biomedicine, 5(5):387–394. http://dx.doi.org/10.1016/s2221-1691(15)30374-9
• 2. Adeneye, A.A., Crooks, P. A., Fadhel-Albayati, Z., Miller, A., Zito, S.W., Adeyemi, O.O. and Agbaje, E.O., 2013. The Novel Antihyperglycaemic Action of Hunteria Umbellata Seed Fractions Mediated Via Intestinal Glucose Uptake Inhibition. African Journal of Traditional, Complementarily and Alternative Medicine, 10(2):189 202. http://dx.doi.org/10.4314/ajtcam.v10i2.3
• 3. Adeneye, A.A. and Adeyemi, O.O., 2009. Hypoglycaemic effects of the aqueous seed extract of Hunteria umbellata in normoglycaemic and glucose and nicotine-induced hyperglycaemic rats. International Journal of Natural Product Resources, 2(1):9-18. http://doi:10.1055/s-0028-1084243
• 4. Adeneye, A.A. and Adeyemi, O.O., 2009a Hypoglycaemic effects of the aqueous seed extract of Hunteria umbellata in normoglycaemic and glucose and nicotine-induced hyperglycaemic rats. International Journal of Natural Product Research, 2(1):9-18. htpps://doi: 10.1016/j.jep.2009.08.037
• 5. Adeneye, A.A. and Adeyemi, O.O., 2009b. Further evaluation of antihyperglycaemic activity of Hunteria umbellata (K. Schum) Hallier f. seed extract in experimental diabetes. Journal of Ethnopharmacology, 126(2): 238-243. htpps://doi: 10.1016/j.jep.2009.08.037
• 6. Adeneye, A.A., Crooks, P. A., Fadhel-Albayati, Z., Miller, A., Zito, S.W., Adeyemi, E.O. Agbaje, 2013. The Novel Antihyperglycaemic Action of Hunteria Umbellata Seed Fractions Mediated Via Intestinal Glucose Uptake Inhibition. African Journal of Traditional, Complement and Alternative Medicine 10(2):189‐202
• 7. Aderele, O.R. Rasaq, A.K. and Momoh, J.O., 2020. Phytochemical Screening, Mathematical Analysis and Antimicrobial Activity of Methanolic Seed Extract of Hunteria Umbellata. European Journal of Medicinal Plants, 1–17. http://dx.doi.org/10.9734/ejmp/2020/v31i1630325
• 8. Adesokan, A. and Akanji, M. 2010., Haematological changes following chronic administratio n of aqueous extract of Enantia Chlorantha to albino rats. Tropical Journal of Health and Science, 17(1). http:// dx.doi.org/10.4314/tjhc.v17i1.52791
• 9. Ashafa, O.T., Yakubu, M.T., Grierson, D.S. and Afolayan, A.J., 2009. Toxicological evaluation of the aqueous extract of Felicia muricata Thunb. leaves in Wistar rats. African Journal of Biotechnology, 8: 949-54. https://doi.org/10.3109/13880200903373664
• 10. Ayinde, O.E., Ojo, V., Adeyina, A.A. and Adesoye, O., 2010. Economics of using Cocoa bean shell as feed supplement for rabbits. Pakistan Journal of Nutrition, 9:195-197. https://doi: 10.3923/pgn.2010.195.197
• 11. Azwanida, N.N., 2015. A Review on the Extraction Methods Use in Medicinal Plants, Principle, Strength and Limitation. Medicinal and Aromatic Plants, 4: 196. https://doi:10.4172/2167- 0412.1000196
• 12. Dacie, J.V. and Lewis, S.M., 1975. Practical Haematology. Churchill Livingstone 629p. New York, 5th ed. https://doi.org/10.1111/bjh.14872 13. Dave, M., Gupta, A.K. and Heernath, P.P., 2019. Correlation between Fasting Blood Sugar Level, HbA1C Level and Serum Lipid Levels in Type 2 Diabetes Mellitus Patients. International Journal of Contemporarily Medical Research, 6(7). http://dx.doi.org/10.21276/ijcmr.2019.6.7.13
• 14. Friedewald, W.T., Levy, R.I. and Fredrickson, D.S., 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clinical Chemistry, 18:499-502. https://doi.org/10.1093/clinchem/18.6.499
• 15. Gurib-Fakim, A., 2006. Medicinal plants: Traditions of yesterday and drugs of tomorrow.
• 16. Han, N., Htoo, H.K.S. and Aung, H., 2012. Determinants of Abnormal Liver Function Tests in Diabetes Patients in Myanmar. International Journal of Diabetes Research, 1(3):36-41. http://dx.doi.org/10.5923/j.diabetes.20120103.02
• 17. Harris, E.H., 2005. Elevated liver function tests in Type 2 Diabetes. Clinical Diabetes, 23(3):115- 119. http://dx.doi.org/10.2337/diaclin.23.3.115
• 18. Igbe, I., Omogbai, E.K.I. and Ozolua, R.I., 2009. Hypoglycaemic activity of aqueous seed extract of Hunteria umbellata in normal and streptozotocin-induced diabetic rats. Pharmaceutical Biology, 47(10):1011-1016. https://doi.org/10.1080/13880200902973803
• 19. Kakade, M.L., Simons, N.R., Liener, I.E. and Lambert, J.W., 1972. Biochemical and nutritional assessment of different varieties of soybeans. Journal of Agricultural and Food Chemistry, 20:87-90. htpps://doi: 10.1021/jf60179a024
• 20. Kao, F.J., 2018. Effect of extracting solvents on antioxidant activities from fruit of Phyllanthus emblica Linn. Medicinal and Aromatic Plants, 07. http://dx.doi.org/10.4172/2167-0412-c1- 018
• 21. Keay, R.W.J., Onochie, C.F.A. and Stanfield, D.P., 1989. Tree of Nigeria (formerly Nigerian trees, 1960, 1964), Oxford University, Oxford, UK 234pp.
• 23. Kyoungho, L., Joohee, H. and Soo-Geun, K., 2014. Increasing risk of diabetes mellitus according to liver function alterations in electronic workers. Journal of Diabetes Investigation, 5:671–676. http://dx.doi.org/10.1111/jdi.12202. 24. Muhd, H.M.I, Siti, B.B., Mohamad, O. and Jamaludin, M., 2012. Protective role of Hibiscus sabdariffa calyx extract against streptozotocin induced sperm damage in diabetic rats. Experimental and Clinical Psychopharmacology, 11:659-669. https:// doi:10.17877/DE290R-5145
• 25. Murray, P., Pitt, B. and Webb, R.C., 1994. Ramipril prevents hypersensitivity to phenylephrine in aorta from streptozotocin-induced diabetic rats. Diabetologia, 37(7): 664–670. http://dx.doi.org/10.1007/s001250050161
• 26. Niranjan, R.M. and Kanaki, S., 2008. Phytochemical Standardization of Herbal Drugs and Polyherbal Formulations. Bioactive Molecules and Medicinal Plants, UK, pp. 349-369. https://doi:10.1007/978-3-540-74603-4_19
• 27. Oboh, G. , Adebayo, A.A. and Ademosun, A.O., 2017. Erection-stimulating, anti-diabetic and antioxidant properties of Hunteria umbellata and Cylicodiscus gabunensis water extractable phytochemicals. Journal of Complementary and Integrated Medicine 15(1). http://dx.doi.org/10.1515/jcim-2016-0164
• 28. Ogbera, A.O., Fasanmade, O.A., Chinenye, S. and Akinlade. A., 2009. Characterization of lipidparameters in diabetes mellitus - a Nigerian report. International Archives of Medicine, 2(19):1-7. http://dx.doi.org/10.1186/1755-7682-2-19
• 29. Onoja, S.O., Udem, S.C. and Anaga, A.O. 2018. Ameliorative effects of Helianthus annuus ag ainst nephrotoxic, cardiac, and haematological disorders in alloxan-induced hyperglycae mia in albino rats. Journal of Veterinary Research, 62(3): 371–377. http://dx.doi.org/10.2478/jvetres-2018-005
• 30. Price, R., 1985. Phytochemical methods - a guide to modern techniques of plant analysis. Physiological Plant Pathology, 27(2):255–256. http://dx.doi.org/10.1016/0048-4059(85)90073-6
• 31. Rees, A.J., 2007. A comparison of the haematological effects of pregnancy on the Han Wistar ra. Toxicology, 240(3): 165–166. http://dx.doi.org/10.1016/j.tox.2007.06.044
• 32. Reitman, S. and Frankel, S., 1957. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. American Journal of Clinical Pathology, 28(1):56-63. http://doi: 10.1093/ajcp/28.1.56
• 33. Reynolds, T. and Sofowora, A., 1984. Medicinal Plants and Traditional Medicine in Africa. Kew Bulletin 39(3):667. Available at: http://dx.doi.org/10.2307/4108615
• 34. Selvaraju, K, Narayanaswamy, T. Thirunavukkarasu and T. Gangaipillai, A., 2013. Anti diabetic effect of methanolic leaf extract of Pongamia pinnata on streptozotocin induced diabetic rats. Journal of Coast Life Medicine, 1(2):113-117. https://doi:10.12980/JCLM.1.2013J9
• 35. Subash-Babu, P., Prabuseenivasan, S. and Ignacimuthu, S.C., 2007. A potential antidiabetic agent. Phytomedicine, 14: 15–22. https://doi: 10.1016/j.phymed.2006.11.005
• 36. Thirumalai,T., Theresa, V.S., Elumalai, E.K. and David, E., 2011. Hypoglycemic effect of Brassica juncea (seeds) on streptozotocin induced diabetic male albino rat. Asian Pacific Journal of Tropical Biomedicine, 4:323-325. http://doi: 10.1016/S2221-1691(11)60052-X
• 37. Trinder, P., 1969. Determination of blood glucose using an oxidase-peroxidase system with a non-carcinogenic chromogen. Journal of Clinical Pathology, 22(2):158-161. https://dx.doi.org/10.1136/jcp.22.2.158
• 38. World Health Organization (WHO). World health statistics. 2011;170. ISBN ISBN: 978- 92-4-256419-8
• 39. Xing-Jiu, H., Yang-Kyu, C., Hyung-Soon, I., Oktay, Y., Euisik, Y. and Hak-Sung, K., 2006. Aspartate Aminotransferase (AST/GOT) and Alanine Aminotransferase (ALT/GPT) Detection Techniques. MDPI, 6: 756-782. https://doi.org/10.3390/s6070756