Research Article
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Phytochemical Investigation, Antioxidant, and Enzyme Inhibitory Activities of Blackberry (Rubus fruticosus) Fruits

Year 2024, Volume: 7 Issue: 1, 34 - 40, 06.07.2024
https://doi.org/10.38093/cupmap.1463141

Abstract

This scientific paper explores the phytochemical composition of blackberry (Rubus fruticosus) fruit and investigates its antioxidant and enzyme inhibitory activities. Blackberry is known for its rich nutritional profile and potential health benefits, making it a subject of interest in the field of functional foods and natural medicine. The study aims to provide valuable insights into the bioactive compounds present in blackberry fruit, their antioxidant properties, and their potential as enzyme inhibitors. According to the results, it was determined that the fruit extracts had strong antioxidant and moderate enzyme inhibition activity. HPLC analysis showed that the fruit extract generally contained o-coumaric acid, procatechin, ellagic acid and quercetin as major components.

Supporting Institution

Selcuk University Scientific Research Foundation

Project Number

SUBAP- 23202037

Thanks

The authors extend their appreciation to the Selcuk University Scientific Research Foundation for funding this research work through the project number: SUBAP- 23202037. We would like to thank research assistant M. Raşit Bakır for his support in the HPLC analysis.

References

  • 1. Arcone, R., D’Errico, A., Nasso, R., Rullo, R., Poli, A., Di Donato, P., & Masullo, M. (2023). Inhibition of Enzymes Involved in Neurodegenerative Disorders and A β 1–40 Aggregation by Citrus limon Peel Polyphenol Extract. Molecules, 28(17), 6332.
  • 2. Bhatia, A., Singh, B., Arora, R., & Arora, S. (2019). In vitro evaluation of the α-glucosidase inhibitory potential of methanolic extracts of traditionally used antidiabetic plants. BMC complementary and alternative medicine, 19, 1-9.
  • 3. Boath, A. S., Grussu, D., Stewart, D., & McDougall, G. J. (2012). Berry polyphenols inhibit digestive enzymes: a source of potential health benefits? Food Digestion, 3, 1-7.
  • 4. Chun, S.-S., Vattem, D. A., Lin, Y.-T., & Shetty, K. (2005). Phenolic antioxidants from clonal oregano (Origanum vulgare) with antimicrobial activity against Helicobacter pylori. Process Biochemistry, 40(2), 809-816.
  • 5. Clarke, G., Ting, K. N., Wiart, C., & Fry, J. (2013). High correlation of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing activity potential and total phenolics content indicates redundancy in use of all three assays to screen for antioxidant activity of extracts of plants from the Malaysian rainforest. Antioxidants, 2(1), 1-10.
  • 6. Dal, S., & Sigrist, S. (2016). The protective effect of antioxidants consumption on diabetes and vascular complications. Diseases, 4(3), 24.
  • 7. Fredes González, C. P., Montenegro Rizzardini, G., Zoffoli, J. P., Santander, F., & Robert Canales, P. S. (2014). Comparison of the total phenolic content, total anthocyanin content and antioxidant activity of polyphenol-rich fruits grown in Chile.
  • 8. Huang, W.-y., Zhang, H.-c., Liu, W.-x., & Li, C.-y. (2012). Survey of antioxidant capacity and phenolic composition of blueberry, blackberry, and strawberry in Nanjing. Journal of Zhejiang University Science B, 13, 94-102.
  • 9. Ibach, B., & Haen, E. (2004). Acetylcholinesterase inhibition in Alzheimer's Disease. Current Pharmaceutical Design, 10(3), 231-251.
  • 10. Jeong, S. H., Ryu, Y. B., Curtis-Long, M. J., Ryu, H. W., Baek, Y. S., Kang, J. E., Lee, W. S., & Park, K. H. (2009). Tyrosinase inhibitory polyphenols from roots of Morus lhou. Journal of agricultural and food chemistry, 57(4), 1195-1203.
  • 11. Lobo, V., Patil, A., Phatak, A., & Chandra, N. (2010). Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy reviews, 4(8), 118.
  • 12. Manoharan, S., Guillemin, G. J., Abiramasundari, R. S., Essa, M. M., Akbar, M., & Akbar, M. D. (2016). The role of reactive oxygen species in the pathogenesis of Alzheimer’s disease, Parkinson’s disease, and Huntington’s disease: a mini review. Oxidative medicine and cellular longevity, 2016.
  • 13. Martins, M. S., Gonçalves, A. C., Alves, G., & Silva, L. R. (2023). Blackberries and mulberries: Berries with significant health-promoting properties. International Journal of Molecular Sciences, 24(15), 12024.
  • 14. Nagatsu, T., Nakashima, A., Watanabe, H., Ito, S., & Wakamatsu, K. (2022). Neuromelanin in Parkinson’s disease: tyrosine hydroxylase and tyrosinase. International Journal of Molecular Sciences, 23(8), 4176.
  • 15. Quettier-Deleu, C., Gressier, B., Vasseur, J., Dine, T., Brunet, C., Luyckx, M., Cazin, M., Cazin, J.-C., Bailleul, F., & Trotin, F. (2000). Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. Journal of ethnopharmacology, 72(1-2), 35-42.
  • 16. Sariburun, E., Şahin, S., Demir, C., Türkben, C., & Uylaşer, V. (2010). Phenolic content and antioxidant activity of raspberry and blackberry cultivars. Journal of food science, 75(4), C328-C335.
  • 17. Šinko, G., Čalić, M., Bosak, A., & Kovarik, Z. (2007). Limitation of the Ellman method: Cholinesterase activity measurement in the presence of oximes. Analytical biochemistry, 370(2), 223-227.
  • 18. Tan, Y., & Chang, S. K. (2017). Digestive enzyme inhibition activity of the phenolic substances in selected fruits, vegetables and tea as compared to black legumes. Journal of Functional Foods, 38, 644-655.
  • 19. Tundis, R., Loizzo, M. R., & Menichini, F. (2010). Natural products as α-amylase and α-glucosidase inhibitors and their hypoglycaemic potential in the treatment of diabetes: an update. Mini reviews in medicinal chemistry, 10(4), 315-331.
  • 20. Wang, H., Gao, X. D., Zhou, G. C., Cai, L., & Yao, W. B. (2008). In vitro and in vivo antioxidant activity of aqueous extract from Choerospondias axillaris fruit. Food Chemistry, 106(3), 888-895.
  • 21. Yang, X., & Kong, F. (2016). Effects of tea polyphenols and different teas on pancreatic α-amylase activity in vitro. LWT-Food Science and Technology, 66, 232-238.
Year 2024, Volume: 7 Issue: 1, 34 - 40, 06.07.2024
https://doi.org/10.38093/cupmap.1463141

Abstract

Project Number

SUBAP- 23202037

References

  • 1. Arcone, R., D’Errico, A., Nasso, R., Rullo, R., Poli, A., Di Donato, P., & Masullo, M. (2023). Inhibition of Enzymes Involved in Neurodegenerative Disorders and A β 1–40 Aggregation by Citrus limon Peel Polyphenol Extract. Molecules, 28(17), 6332.
  • 2. Bhatia, A., Singh, B., Arora, R., & Arora, S. (2019). In vitro evaluation of the α-glucosidase inhibitory potential of methanolic extracts of traditionally used antidiabetic plants. BMC complementary and alternative medicine, 19, 1-9.
  • 3. Boath, A. S., Grussu, D., Stewart, D., & McDougall, G. J. (2012). Berry polyphenols inhibit digestive enzymes: a source of potential health benefits? Food Digestion, 3, 1-7.
  • 4. Chun, S.-S., Vattem, D. A., Lin, Y.-T., & Shetty, K. (2005). Phenolic antioxidants from clonal oregano (Origanum vulgare) with antimicrobial activity against Helicobacter pylori. Process Biochemistry, 40(2), 809-816.
  • 5. Clarke, G., Ting, K. N., Wiart, C., & Fry, J. (2013). High correlation of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing activity potential and total phenolics content indicates redundancy in use of all three assays to screen for antioxidant activity of extracts of plants from the Malaysian rainforest. Antioxidants, 2(1), 1-10.
  • 6. Dal, S., & Sigrist, S. (2016). The protective effect of antioxidants consumption on diabetes and vascular complications. Diseases, 4(3), 24.
  • 7. Fredes González, C. P., Montenegro Rizzardini, G., Zoffoli, J. P., Santander, F., & Robert Canales, P. S. (2014). Comparison of the total phenolic content, total anthocyanin content and antioxidant activity of polyphenol-rich fruits grown in Chile.
  • 8. Huang, W.-y., Zhang, H.-c., Liu, W.-x., & Li, C.-y. (2012). Survey of antioxidant capacity and phenolic composition of blueberry, blackberry, and strawberry in Nanjing. Journal of Zhejiang University Science B, 13, 94-102.
  • 9. Ibach, B., & Haen, E. (2004). Acetylcholinesterase inhibition in Alzheimer's Disease. Current Pharmaceutical Design, 10(3), 231-251.
  • 10. Jeong, S. H., Ryu, Y. B., Curtis-Long, M. J., Ryu, H. W., Baek, Y. S., Kang, J. E., Lee, W. S., & Park, K. H. (2009). Tyrosinase inhibitory polyphenols from roots of Morus lhou. Journal of agricultural and food chemistry, 57(4), 1195-1203.
  • 11. Lobo, V., Patil, A., Phatak, A., & Chandra, N. (2010). Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy reviews, 4(8), 118.
  • 12. Manoharan, S., Guillemin, G. J., Abiramasundari, R. S., Essa, M. M., Akbar, M., & Akbar, M. D. (2016). The role of reactive oxygen species in the pathogenesis of Alzheimer’s disease, Parkinson’s disease, and Huntington’s disease: a mini review. Oxidative medicine and cellular longevity, 2016.
  • 13. Martins, M. S., Gonçalves, A. C., Alves, G., & Silva, L. R. (2023). Blackberries and mulberries: Berries with significant health-promoting properties. International Journal of Molecular Sciences, 24(15), 12024.
  • 14. Nagatsu, T., Nakashima, A., Watanabe, H., Ito, S., & Wakamatsu, K. (2022). Neuromelanin in Parkinson’s disease: tyrosine hydroxylase and tyrosinase. International Journal of Molecular Sciences, 23(8), 4176.
  • 15. Quettier-Deleu, C., Gressier, B., Vasseur, J., Dine, T., Brunet, C., Luyckx, M., Cazin, M., Cazin, J.-C., Bailleul, F., & Trotin, F. (2000). Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. Journal of ethnopharmacology, 72(1-2), 35-42.
  • 16. Sariburun, E., Şahin, S., Demir, C., Türkben, C., & Uylaşer, V. (2010). Phenolic content and antioxidant activity of raspberry and blackberry cultivars. Journal of food science, 75(4), C328-C335.
  • 17. Šinko, G., Čalić, M., Bosak, A., & Kovarik, Z. (2007). Limitation of the Ellman method: Cholinesterase activity measurement in the presence of oximes. Analytical biochemistry, 370(2), 223-227.
  • 18. Tan, Y., & Chang, S. K. (2017). Digestive enzyme inhibition activity of the phenolic substances in selected fruits, vegetables and tea as compared to black legumes. Journal of Functional Foods, 38, 644-655.
  • 19. Tundis, R., Loizzo, M. R., & Menichini, F. (2010). Natural products as α-amylase and α-glucosidase inhibitors and their hypoglycaemic potential in the treatment of diabetes: an update. Mini reviews in medicinal chemistry, 10(4), 315-331.
  • 20. Wang, H., Gao, X. D., Zhou, G. C., Cai, L., & Yao, W. B. (2008). In vitro and in vivo antioxidant activity of aqueous extract from Choerospondias axillaris fruit. Food Chemistry, 106(3), 888-895.
  • 21. Yang, X., & Kong, F. (2016). Effects of tea polyphenols and different teas on pancreatic α-amylase activity in vitro. LWT-Food Science and Technology, 66, 232-238.
There are 21 citations in total.

Details

Primary Language English
Subjects Pharmacognosy
Journal Section Research Articles
Authors

Ali Hasan Saqee Saqee 0009-0007-2574-5593

Nuraniye Eruygur 0000-0002-4674-7009

Project Number SUBAP- 23202037
Early Pub Date May 25, 2024
Publication Date July 6, 2024
Submission Date April 2, 2024
Acceptance Date May 11, 2024
Published in Issue Year 2024 Volume: 7 Issue: 1

Cite

APA Saqee, A. H. S., & Eruygur, N. (2024). Phytochemical Investigation, Antioxidant, and Enzyme Inhibitory Activities of Blackberry (Rubus fruticosus) Fruits. Current Perspectives on Medicinal and Aromatic Plants, 7(1), 34-40. https://doi.org/10.38093/cupmap.1463141

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